SARS-CoV-2 Detection Strategies

This is the page about the kits/methods to detect SARS-Cov-2.

To detect this virus, in the market there are two different strategies: 1) direct detection by using its RNA and protein 2) indirect detection by targetting IgM or IgG against the virus

The figure below illustrates when these two different strategies can be used.

As seen above each scenario has a different scientific/diagnostic meaning

To see the big picture we need to have different commercial kits/methods.

1. Direct Detection Methods (DDM)

The direct detection methods aim to detect the active infection or SARS-CoV-2 itself. We can classify them as Nucleic Acid (NA) Based Methods (NBM) and Protein-Based Methods (PBM).

1.1. Nucleic Acid-Based Methods

1.1.1. PCR Related Methods

Nowadays, the most famous NBMs are RT-PCR derivatives which are RT-qPCR and RT-ddPCRs.

1.1.2. Other Methods

Recently, Abbott launched a novel Covid-19 test kits. The kits use isothermal amplification to detect RdRp gene of SARS CoV-2 virus. Although the company does not give detailed information about the technique, I think they use LAMP technology.

1.2. Antigen Detection (Protein-Based Methods):

Although there are a few RNA strands in each virus, the number of protein molecules is enormous. In addition, RNA isolation is not necessary, which is highly important for labs which has weak or limited supply chain to reach RNA isolation kits.




Print Friendly, PDF & Email