773.702.5508

Gordon Center for Integrative Science
929 E. 57th Street
Room W-102
Chicago, IL 60637
773.702.5508

The Biophysics core is directed by Dr. Ronald Rock and Dr. Elena Solomaha and located in the Gordon Center for Integrative Science. The core provides a complete set of instruments for quantitative characterization of macromolecules and relevant biological systems.  Laser light scattering, fluorometry, CD spectrometry, and spectrophotometry determine absolute properties of macromolecules: weight, size, hydrodynamic radius, composition, polarization, intramolecular distances, and the presence of certain functional groups.  These complementary techniques, coupled with thermodynamic and interactional data from bulk techniques such as surface plasmon resonance (SPR), spectroscopy, fluorescence intensity and calorimetry, yield a comprehensive, robust, and reliable portrait of the molecule of interest and its biological activity. The facility also offers instrumentation, such as the multimode plate reader and Seahorse extracellular flux analyzer, for direct measurement of cellular and macromolecular activity. The Biophysics Core Facility instruments:

Extracellular Flux Analyzer: Seahorse ECF, Agilent

Isothermal Titration Calorimetry: ITC 200, Malvern  

Circular Dichroism Spectrometer:  Jasco J-1500, Jasco

Surface Plasmon Resonance.  Biacore 8K 

Static/Dynamic Light Scattering: NanoStar, DynoPro, DAWN HELEOS, Water Technology

Fluorescence Spectroscopy: Flurolog-3 Yobin Yvon-Horiba, Horiba; Synergy Neo HST, Agilent

Luminescence: Synergy Neo HST, Agilent

Spectroscopy: Synergy Neo HST, Agilent

Molecular Imaging: ChemiDoc MP Imager, Bio-Rad; ChemiDoc MP Imaging Systems, Bio-Rad

Core may be identified in publications as The University of Chicago Biophysics Core Facility (RRID:SCR_017915).