To access a microscope, click the New User button above to go to our New User Training Checklist.Trained users have 24/7 access to the facility and are given permission to schedule their own microscope sessions through our online scheduling software
MICROSCOPES BY TYPE AND FUNCTION
- Confocal
- Histology
- Lightsheet
- Live Cell Imaging
- Superresolution
- TIRFM
- Widefield
- Whole Slide Scanning
- 2-photon
3i Lattice Lightsheet Bessel Beam Illumination microscope
Miltenyi LaVision Ultramicroscope II large format upright lightsheet
Zeiss Lightsheet Z.1 – no longer available, moved to Organismal Biology and Anatomy CDIF facility 4/6/2022
The following microscopes are optimized for live cell imaging. They cover several different microscope types and are also listed by type in other tabs.
Chamber slide users see our chamber slide use warning before starting your cultures!
Olympus IX81 inverted widefield microscope
Zeiss AxioObserver 7 inverted widefield microscope
Olympus VivaView in-incubator widefield microscope
Olympus “live cell” DSU inverted spinning disk confocal
Leica SP5 2-photon inverted laser scanning confocal
Leica SP5 STED inverted laser scanning confocal
The following microscopes are capable of superresolution imaging. They cover several different microscope and superresolution types and are also listed by type in other tabs
Leica GSD/TIRFM inverted ground state depletion superresolution with TIRF
Leica SP8 inverted laser scanning confocal with white light laser, 3D STED and FLIM
Leica SP5 STED inverted laser scanning confocal
The following microscopes are optimized for mosaic scanning of large, fixed specimens on slides. They cover several different microscope types (histology, widefield fluorescence and confocal fluorescence) and are also listed by type in other tabs
Olympus VS200 whole slide scanner (drop off service)
CRi Pannoramic SCAN 40x whole slide scanner (drop off service)
CRi Pannoramic MIDI 20x whole slide scanner (drop off service)
Caliber I.D. RS-G4 – upright large format laser scanning confocal
MAP OF KCBD 1250
EQUIPMENT STATUS
Green: No issues. Yellow: Can be used with minor issues, see notes below. Red: Cannot be used, see notes below.
CONFOCALS
- Leica SP5_2-photon 100%
- Marianas 100%
- SoRa Marianas 100%
- Stellaris 8 100%
- Leica SP8 100%
- Leica SP5_STED 100%
- Caliber 100%
- “Live sample” DSU 100%
ANALYSIS STATIONS
- Arivis workstation 100%
- Imaris workstation 100%
- Huygens workstation 100%
- GSD/VS200 offline workstation 100%
LIGHTSHEETS
- Lattice Lightsheet 100%
- LaVision Ultramicroscope Lightsheet 100%
SCANNER DROP-OFF SERVICE
- Olympus VS200 Slideview 100%
- CRi Pannoramic MIDI 20x 100%
WIDEFIELDS
- GSD/TIRFM 100%
- KCBD IX81 100%
- AxioObserver 7 100%
- VivaView 100%
- Stereomicroscope 100%
- Axioskop 100%
Marianas Spinning Disk Confocal – March 1st, 2024 – The motorized ASI stage has failed again. The stage has been repaired and will be installed on Wednesday March 7th. Updates pending.
Stellaris8 – March 1st, 2024 – The microscope should be up and running. If you run into an issue where you can see fluorescence through the epi-fluorescent microscope (i.e. the nosepeice) but when you switch to the laser-scanning-confocal you are no longer able to see your image (using imaging settings that have previously worked), please contact Microscopy Core personnel.
Lattice Lightsheet – March 1, 2024 – The objective lens needs to be replaced. We are working with 3i for this repair however the microscope is currently inoperable. Updates pending.
SP5 2-Photon – March 1, 2024 – The hyd D2 detector is failing. We recommend avoiding this detector is possible. For explanation of SP5 2-photon status and SP5 STED long-term deficits, please see their microscope page descriptions.