Integrated Light Microscopy Core

To access a microscope, contact the Technical Director listed in the description and schedule a hands-on training session. Trained users have 24/7 access to the facility and are given permission to schedule their own microscope sessions through our online scheduling software

KCBD 1250 MAIN SITE

The following microscopes are optimized for live cell imaging. They cover several different microscope types and are also listed by type in other tabs.

Chamber slide users see our chamber slide use warning before starting your cultures!

Olympus IX81 inverted widefield microscope

Olympus VivaView in-incubator widefield microscope

Olympus “live cell” DSU inverted spinning disk confocal

Leica SP5 STED inverted laser scanning confocal

Zeiss Lightsheet Z.1

3i Lattice Lightsheet with bessel beam illumination

The following microscopes are capable of superresolution imaging. They cover several different microscope and superresolution types and are also listed by type in other tabs

Leica GSD/TIRFM inverted ground state depletion superresolution with TIRF

Leica SP8 inverted laser scanning confocal with white light laser, 3D STED and FLIM

Leica SP5 STED inverted laser scanning confocal

 

The following microscopes are optimized for mosaic scanning of large, fixed specimens on slides. They cover several different microscope types (histology, widefield fluorescence and confocal fluorescence) and are also listed by type in other tabs

CRi Pannoramic SCAN 40x whole slide scanner (drop off service)

CRi Pannoramic MIDI 20x whole slide scanner (drop off service)

Caliber I.D. RS-G4 – upright large format laser scanning confocal

ABBOTT 129 SATELLITE

MAP OF KCBD 1250

MAP OF ABBOTT 129

EQUIPMENT STATUS

Green: No issues. Yellow: Can be used with minor issues, see notes below. Red: Cannot be used, see notes below.

ABBOTT MICROSCOPES
  • Leica SP5_2-photon 100% 100%
  • Marianas 100% 100%
  • Axiovert 135 100% 100%
  • Leitz 100% 100%

ANALYSIS STATIONS

  • Arivis workstation 100% 100%
  • Imaris workstation 100% 100%
  • Huygens workstation 100% 100%
  • GSD/VS200 offline workstation 100% 100%

KCBD CONFOCALS

  • Stellaris 8 100% 100%
  • Leica SP8 100% 100%
  • Leica SP5_STED 100% 100%
  • Caliber ID 100% 100%
  • “Live sample” DSU 100% 100%
  • “Fixed sample” DSU 100% 100%

KCBD LIGHTSHEETS

  • Lightsheet Z.1 100% 100%
  • Lattice Lightsheet 100% 100%
  • LaVision Ultramicroscope Lightsheet 100% 100%

SCANNER DROP-OFF SERVICE

  • Olympus VS200 Slideview 100% 100%
  • CRi Pannoramic SCAN 40x 100% 100%
  • CRi Pannoramic MIDI 20x 100% 100%

KCBD WIDEFIELDS

  • GSD/TIRFM 100% 100%
  • IX81 100% 100%
  • Axiovert 200 100% 100%
  • VivaView 100% 100%
  • Stereomicroscope 100% 100%
  • Axioskop 100% 100%

Olympus IX81 widefield – DO NOT SHUT DOWN OR RESTART WINDOWS ON THIS SYSTEM. Due to a software bug, shutting down or restarting Windows results in loss of the camera software. Upon restart the camera will not run until software is re-installed by an admin. Signs on the monitor will remind you of this warning.

Leica SP5-STED 9/24/2021 – HyD4 detector is dead.  Protocols will have to be re-formatted to work around this detector until it can be replaced.

OlympusVivaView – As of 9/17/2021 the mag changer gears have worn out and are not working. Only 20x magnification is available. We’re working with Olympus to see if we can get parts and repair the system.

OlympusVS200 As of 9/24/2021  – certain trays are not loading correctly, making setup a little more challenging.  Slides can still be scanned. Service is scheduled for next week.