Integrated Light Microscopy Core

To access a microscope, click the New User button above to go to our New User Training Checklist.Trained users have 24/7 access to the facility and are given permission to schedule their own microscope sessions through our online scheduling software

MICROSCOPES BY TYPE AND FUNCTION

The following microscopes are capable of superresolution imaging. They cover several different microscope and superresolution types and are also listed by type in other tabs

Leica GSD/TIRFM inverted ground state depletion superresolution with TIRF

Leica SP8 inverted laser scanning confocal with white light laser, 3D STED and FLIM

Leica SP5 STED inverted laser scanning confocal

 

The following microscopes are optimized for mosaic scanning of large, fixed specimens on slides. They cover several different microscope types (histology, widefield fluorescence and confocal fluorescence) and are also listed by type in other tabs

Olympus VS200 whole slide scanner (drop off service)

CRi Pannoramic SCAN 40x whole slide scanner (drop off service)

CRi Pannoramic MIDI 20x whole slide scanner (drop off service)

Caliber I.D. RS-G4 – upright large format laser scanning confocal

MAP OF KCBD 1250

EQUIPMENT STATUS

Green: No issues. Yellow: Can be used with minor issues, see notes below. Red: Cannot be used, see notes below.

CONFOCALS

  • Leica SP5_2-photon 100% 100%
  • Marianas 100% 100%
  • SoRa Marianas 100% 100%
  • Stellaris 8 100% 100%
  • Leica SP8 100% 100%
  • Leica SP5_STED 100% 100%
  • Caliber 100% 100%
  • “Live sample” DSU 100% 100%

ANALYSIS STATIONS

  • Arivis workstation 100% 100%
  • Imaris workstation 100% 100%
  • Huygens workstation 100% 100%
  • GSD/VS200 offline workstation 100% 100%

LIGHTSHEETS

  • Lattice Lightsheet 100% 100%
  • LaVision Ultramicroscope Lightsheet 100% 100%

SCANNER DROP-OFF SERVICE

  • Olympus VS200 Slideview 100% 100%
  • CRi Pannoramic MIDI 20x 100% 100%

WIDEFIELDS

  • GSD/TIRFM 100% 100%
  • KCBD IX81 100% 100%
  • AxioObserver 7 100% 100%
  • VivaView 100% 100%
  • Stereomicroscope 100% 100%
  • Axioskop 100% 100%

Marianas Spinning Disk Confocal – April 29, 2024 – The motorized ASI stage has been repaired and is ready for use!

Stellaris8 – March 1st, 2024 – The microscope should be up and running. If you run into an issue where you can see fluorescence through the epi-fluorescent microscope (i.e. the nosepeice) but when you switch to the laser-scanning-confocal you are no longer able to see your image (using imaging settings that have previously worked), please contact Microscopy Core personnel.

Lattice Lightsheet – June 21, 2024 – The objective lens has been replaced! The microscope is back up and running, however the finder camera feature is not currently working. Updates pending.

SP5 2-Photon – March 1, 2024 – The hyd D2 detector is failing. We recommend avoiding this detector is possible. For explanation of SP5 2-photon status and SP5 STED long-term deficits, please see their microscope page descriptions.