Integrated Light Microscopy Core

To access a microscope, click the New User button above to go to our New User Training Checklist.Trained users have 24/7 access to the facility and are given permission to schedule their own microscope sessions through our online scheduling software

MICROSCOPES BY TYPE AND FUNCTION

The following microscopes are capable of superresolution imaging. They cover several different microscope and superresolution types and are also listed by type in other tabs

Leica GSD/TIRFM inverted ground state depletion superresolution with TIRF

Leica SP8 inverted laser scanning confocal with white light laser, 3D STED and FLIM

Leica SP5 STED inverted laser scanning confocal

 

The following microscopes are optimized for mosaic scanning of large, fixed specimens on slides. They cover several different microscope types (histology, widefield fluorescence and confocal fluorescence) and are also listed by type in other tabs

Olympus VS200 whole slide scanner (drop off service)

CRi Pannoramic SCAN 40x whole slide scanner (drop off service)

CRi Pannoramic MIDI 20x whole slide scanner (drop off service)

Caliber I.D. RS-G4 – upright large format laser scanning confocal

MAP OF KCBD 1250

EQUIPMENT STATUS

Green: No issues. Yellow: Can be used with minor issues, see notes below. Red: Cannot be used, see notes below.

CONFOCALS

  • Leica SP5_2-photon 100% 100%
  • Marianas 100% 100%
  • SoRa Marianas 100% 100%

ANALYSIS STATIONS

  • Arivis workstation 100% 100%
  • Imaris workstation 100% 100%
  • Huygens workstation 100% 100%
  • GSD/VS200 offline workstation 100% 100%
  • Stellaris 8 100% 100%
  • Leica SP8 100% 100%
  • Leica SP5_STED 100% 100%
  • Caliber 100% 100%
  • “Live sample” DSU 100% 100%

LIGHTSHEETS

  • Lattice Lightsheet 100% 100%
  • LaVision Ultramicroscope Lightsheet 100% 100%

SCANNER DROP-OFF SERVICE

  • Olympus VS200 Slideview 100% 100%
  • CRi Pannoramic MIDI 20x 100% 100%

WIDEFIELDS

  • GSD/TIRFM 100% 100%
  • KCBD IX81 100% 100%
  • AxioObserver 7 100% 100%
  • VivaView 100% 100%
  • Stereomicroscope 100% 100%
  • Axioskop 100% 100%

Leica SP5 STED – August 8, 2023 – The 592 depletion laser on this system is no longer functional.  We do not have a service contract for this end-of-life system, so we will not be replacing this laser. Anyone in need of confocal imaging with DAPI can use the SP5 2-photon, SP8 or Stellaris.  Anyone in need of super-resolution imaging has likely already moved to the SP8 3 color 3D STED system.  The rest of the system continues to function, so confocal with green, red and far red fluorophores is still possible.

Leica SP5 STED – October 18, 2023 – We swapped this Argon (488nm) laser with the one from the SP5 2-photon which was starting to malfunction. We recommend keeping the power level between standby and 20% (under configurations->laser) to decrease the chances of the laser malfunction and prolong the life of the laser.  We do not know how long this laser will last, but we do know if the malfunction becomes more serious we will have to retire this microscope (see no service contract for this end-of-life system above).

Leica SP5 2-photon – October 18, 2023  Leica swapped the Argon laser here with the one on the SP5 STED, so this microscope is back to full function. We recommend keeping the power level low (between standby and 20% under configurations->laser) to prolong the life of the laser.

Leica SP5 2 Photon – January 3, 2024 – The chiller for the MaiTai multiphoton laser has failed therefore the MaiTai is currently out of service. The rest of the Leica SP5 2-photon microscope is working normally for now, so if your project does not require excitation at longer wavelengths (700nm+) it can continue. This includes intravital imaging without the multiphoton laser.

 Stellaris8 – February 8, 2024 – The scan head has been replaced! If you run into an issue where you can see fluorescence through the epi-fluorescent microscope (i.e. the nosepeice) but when you switch to the laser-scanning-confocal you are no longer able to see your image (using imaging settings that have previously worked), please contact Microscopy Core personnel.

AxioObserver7 – February 8, 2024 – The joystick has been replaced! This should fix the issue of the stage drifting to the left, however if you encounter this problem, please contact microscopy core staff.

Lattice Lightsheet – February 8, 2024 – 3i will be performing preventative maintenance February 13th including aligning the lasers. Updates pending.

Marianas Spinning Disk Confocal – February 8, 2024 – The motorized ASI stage has failed again. 3i has been contacted and we are coordinating repairs, which may include a loaner stage or a manual temporary substitution. Updates pending.