Integrated Light Microscopy Core

To access a microscope, contact the Technical Director listed in the description and schedule a hands-on training session. Trained users have 24/7 access to the facility and are given permission to schedule their own microscope sessions through our online scheduling software

KCBD 1250 MAIN SITE

The following microscopes are optimized for live cell imaging. They cover several different microscope types and are also listed by type in other tabs.

Chamber slide users see our chamber slide use warning before starting your cultures!

Olympus IX81 inverted widefield microscope

Olympus VivaView in-incubator widefield microscope

Olympus “live cell” DSU inverted spinning disk confocal

Leica SP5 STED inverted laser scanning confocal

Zeiss Lightsheet Z.1

3i Lattice Lightsheet with bessel beam illumination

The following microscopes are capable of superresolution imaging. They cover several different microscope and superresolution types and are also listed by type in other tabs

Leica GSD/TIRFM inverted ground state depletion superresolution with TIRF

Leica SP8 inverted laser scanning confocal with white light laser, 3D STED and FLIM

Leica SP5 STED inverted laser scanning confocal

 

The following microscopes are optimized for mosaic scanning of large, fixed specimens on slides. They cover several different microscope types (histology, widefield fluorescence and confocal fluorescence) and are also listed by type in other tabs

CRi Pannoramic SCAN 40x whole slide scanner (drop off service)

CRi Pannoramic MIDI 20x whole slide scanner (drop off service)

Caliber I.D. RS-G4 – upright large format laser scanning confocal

ABBOTT 129 SATELLITE

MAP OF KCBD 1250

MAP OF ABBOTT 129

EQUIPMENT STATUS

Green: No issues. Yellow: Can be used with minor issues, see notes below. Red: Cannot be used, see notes below.

ABBOTT MICROSCOPES
  • Leica SP5_2-photon 100% 100%
  • Marianas 100% 100%
  • Axiovert 135 100% 100%
  • Leitz 100% 100%
ANALYSIS STATIONS
  • Arivis workstation 100% 100%
  • Imaris workstation 100% 100%
  • Huygens workstation 100% 100%
  • GSD offline workstation 100% 100%

KCBD CONFOCALS

  • Stellaris 8 100% 100%
  • Leica SP8 100% 100%
  • Leica SP5_STED 100% 100%
  • Caliber ID 100% 100%
  • “Live sample” DSU 100% 100%
  • “Fixed sample” DSU 100% 100%

KCBD LIGHTSHEETS

  • Lightsheet Z.1 100% 100%
  • Lattice Lightsheet 100% 100%
  • LaVision Ultramicroscope Lightsheet 100% 100%

SCANNER DROP-OFF SERVICE

  • Pannoramic SCAN 40x 100% 100%
  • Pannoramic MIDI 20x 100% 100%

KCBD WIDEFIELDS

  • GSD/TIRFM 100% 100%
  • IX81 100% 100%
  • Axiovert 200 100% 100%
  • VivaView 100% 100%
  • Stereomicroscope 100% 100%
  • Axioskop 100% 100%

Leica SP8 – On November 24,2020 HyD2 and HyD4 were upgraded to chilled SMD HyD detectors. Existing sequences will still load, but laser power and gain settings for sequence steps involving those detectors may need to be reduced to avoid overloading the detectors. The Transmitted light detector was replaced on December 7, 2020 so sequences using that detector may also need adjustment in order to work properly.