Cellular Screening Center

Technology

 

The CSC lab space houses a BioBubble softwall cleanroom area for the screens. It is certified to be ISO 5 cleanroom standard. This enclosure ensures a high degree of confidence in assays due to the vastly reduced risk of contamination in this environment.

Robotic Deck

The CSC robotic deck includes a workstation robot for sample prep, plate design and around the clock screens. It is integrated with 3 readers, 4 liquid handlers and 2 storage spaces.

Thermo Scientific Spinnaker Robotic Arm

The Spinnaker robot is able to provide self-correcting precision and sample tracking with its built-in machine vision system. It has 4-axis to reach for a variety positions as needed. The Momentum automation software allows the ease of integration of various workflows on demand.

Readers

There are 4 readers in the CSC enabling multiple different reads within a single screen. All readers are integrated with the Thermo F3 robotic arm.

BioTek Synergy NEO2
Multi-mode reader for absorbance, luminescence, fluorescence intensity, fluorescence polarization and time-resolved fluorescence. It also has a new feature – Alpha detection. The Alpha technology allows for AlphaScreen and AlphaLISA which are used to measure a wide range of biological molecules and interactions in a high throughput manner without the need for washing and diluting.
Molecular Devices ImageXpress Microscope

This automated fluorescent imaging system is capable of fixed or live-cell imaging in multi-well plates. It is equipped with 7 filter sets and 4 objectives (4X, 10x, 20X, and 40X).

Available filters:

Excitation (nm)* Emission (nm)* Example of supported dyes/ proteins
377/50 477/60 DAPI, Hoechst, Pacific Blue
438/24 483/32 CFP, AmCyan, SYTOX Blue
472/30 520/35 GFP, EGFP
482/35 536/40 FITC, Alexa Fluor 488, Calcein, Mito Tracker Green
500/24 542/27 YFP, Eosin, Rhodamine 123
543/22 593/40 TRITC, Alexa Fluor 532 & 546
562/40 624/40 Texas Red, mCherry, Alexa Fluor 568 & 594, Mito Tracker Red

*Center/bandwidth

TTP Labtech Acumen Explorer

Laser-based (488nm) fluorescent microplate cytometer enables whole plate reads in just 10 minutes regardless of plate type. By combining the power of rapid whole well data collection with a CCD imager, this microplate cytometer triumphs over traditional flow cytometer for high throughput screens. It also offers high throughput, high content analysis without generating terabytes of information, making it ideal for use in hit identification and hit-to-lead optimization screens.

Available filters:

Excitation (nm) Emission (nm) Example of supported dyes/ proteins
488 500 – 530 GFP, FITC, Alexa Fluor 488, Calcein, Mito Tracker Green
488 575 – 640 PE, Propidium Iodide, dTomato

Sartorius/Essen BioScience IncuCyte S3
Live-cell imaging system provides real-time automated measurements of the dynamic changes and interactions of cell monolayer as well as spheroids directly in a plate or a flask inside a standard incubator. 4X, 10X and 20X objectives plus phase contrast, green (Ex 440-480/ Em 504-544nm) and red (Ex 565-605/ Em 625-705nm) fluorescent channel are available for independent and continuous imaging.

Liquid Handlers

All liquid handlers except the Tecan Freedom EVO 200 are integrated with the Thermo Scientific Spinnaker robotic arm.

Tecan Freedom EVO 100
Includes an arm with eight tips, each of which can move independently to construct cherry-picked plates. Capable of dispensing to 96, 384, and 1536 well plates. The low volume capabilities of this instrument also allow for the dispensing of low volumes of precious reagents without significantly decreasing speed.

Tecan Freedom EVO 200
This all-in-one re-array and plate replication robot includes an eight tip arm and a 384 well head for plate replication as well as a plate manipulator arm for moving plates across its large deck.

PerkinElmer Janus G3
This Modular Dispense Technology™ (MDT) workstation is equipped with interchangeable 96 well and 384 well heads that are capable of multi-channel dispensing into 96, 384, and 1536-well plates. With the addition of MDT Pin Tool, the workstation can pipette volumes as low as 50nL up to 200mL. Maximum 15 deck positions can be accommodated in the workstation allowing rapid plate replication, whole plate transfers, and multi-steps reagent additions.

Thermo MultiDrop Combi

Three of these instruments are integral for bulk reagent and cell dispensing. They are an excellent combination of robust design and versatility, dispensing 0.5-2500 μl to plates from 6 to 1536 wells and from heights of 5 to 50 mm. 100 μl can be dispensed into a 96 well plate in 10 seconds. They have a newly updated design with an LCD interface and are highly robust instruments with low CVs, high precision, and high throughput speeds. Shown in background video.

Thermo Cytomat 6001
This incubator has a 189 plate capacity and temperature range of +10-50 °C. It is located below the robot deck to optimize deck space but within the reach of the robotic arm.