The CSC lab space houses a BioBubble softwall cleanroom area for the screens. It is certified to be ISO 5 cleanroom standard. This enclosure ensures a high degree of confidence in assays due to the vastly reduced risk of contamination in this environment.
The CSC robotic deck includes a workstation robot for sample prep, plate design and around the clock screens. It is integrated with 3 readers, 4 liquid handlers and 2 storage spaces.
Thermo Scientific Spinnaker Robotic Arm
The Spinnaker robot is able to provide self-correcting precision and sample tracking with its built-in machine vision system. It has 4-axis to reach for a variety positions as needed. The Momentum automation software allows the ease of integration of various workflows on demand.
There are 4 readers in the CSC enabling multiple different reads within a single screen. All readers are integrated with the Thermo F3 robotic arm.
BioTek Synergy NEO2
Molecular Devices ImageXpress Microscope
This automated fluorescent imaging system is capable of fixed or live-cell imaging in multi-well plates. It is equipped with 7 filter sets and 4 objectives (4X, 10x, 20X, and 40X).
|Excitation (nm)*||Emission (nm)*||Example of supported dyes/ proteins|
|377/50||477/60||DAPI, Hoechst, Pacific Blue|
|438/24||483/32||CFP, AmCyan, SYTOX Blue|
|482/35||536/40||FITC, Alexa Fluor 488, Calcein, Mito Tracker Green|
|500/24||542/27||YFP, Eosin, Rhodamine 123|
|543/22||593/40||TRITC, Alexa Fluor 532 & 546|
|562/40||624/40||Texas Red, mCherry, Alexa Fluor 568 & 594, Mito Tracker Red|
TTP Labtech Acumen Explorer
Laser-based (488nm) fluorescent microplate cytometer enables whole plate reads in just 10 minutes regardless of plate type. By combining the power of rapid whole well data collection with a CCD imager, this microplate cytometer triumphs over traditional flow cytometer for high throughput screens. It also offers high throughput, high content analysis without generating terabytes of information, making it ideal for use in hit identification and hit-to-lead optimization screens.
|Excitation (nm)||Emission (nm)||Example of supported dyes/ proteins|
|488||500 – 530||GFP, FITC, Alexa Fluor 488, Calcein, Mito Tracker Green|
|488||575 – 640||PE, Propidium Iodide, dTomato|
Sartorius/Essen BioScience IncuCyte S3
All liquid handlers except the Tecan Freedom EVO 200 are integrated with the Thermo Scientific Spinnaker robotic arm.
Tecan Freedom EVO 100
Tecan Freedom EVO 200
PerkinElmer Janus G3
Thermo MultiDrop Combi
Three of these instruments are integral for bulk reagent and cell dispensing. They are an excellent combination of robust design and versatility, dispensing 0.5-2500 μl to plates from 6 to 1536 wells and from heights of 5 to 50 mm. 100 μl can be dispensed into a 96 well plate in 10 seconds. They have a newly updated design with an LCD interface and are highly robust instruments with low CVs, high precision, and high throughput speeds. Shown in background video.