We provide Sanger sequencing via capillary electrophoresis.

Users provide samples (plasmid DNA, phage or PCR product) at a standardized concentration and, if necessary, custom primers. Core staff process samples by:

1. Preparing cycle sequencing reactions with BigDye v3.1
2. Cleaning sequencing products via sephadex columns made in-house
3. Running capillary electrophoresis on one of our two ABI 3730xl DNA Analyzers
4. Quality checking sequencing runs
5. Delivering the sequence data to users via the OSRF FTP Data Server

In the past the core has also provided fragment analysis (genotyping) and colony picking services. We are working toward restoring these services. In the meantime we provide fragment analysis on a case-by-case basis–please reach out by email with inquiries.