Sanger (DNA) Sequencing Core

Services, Fees, & Payment

Services

We currently offer Sanger sequencing by capillary electrophoresis. The service type is determined by the number of reactions in your order and primer addition needs. Inquire by email if you are interested in fragment analysis by capillary electrophoresis.

Sequencing reactions

  • Each sequencing reaction can only include one sample and one primer
  • Request multiple reactions to sequence the same sample with multiple primers

Low Throughput Sequencing

  • Any orders that includes 7 or fewer reactions
  • Orders of up to 32 reactions with user-supplied primer submitted separately
  • Samples may be submitted in 1.5mL tubes or strip tubes

High Throughput Sequencing

  • Orders of 8 or more reactions to be sequenced with core-supplied primers
  • Orders of 8 or more reactions with user-supplied primers premixed into sample prior to submission
  • Samples may be submitted in strip tubes or 96-well microplates

Ultra-hi Sequencing

  • Orders of 88 or more reactions to be sequenced with core-supplied primers
  • Orders of 88 or more reactions with non-core-supplied primers premixed into sample prior to submission
  • Samples may be submitted in strip tubes or 96-well microplates
Fees
Service name Unit Internal rate External rate
Low-throughput sequencing each $4.50 $6.75
High-throughput sequencing each $1.85 $2.78
Ultra-hi sequencing each $1.20 $1.80
Fragment analysis* each $1.30 $1.95
Glycerol Stock Prep** each $1.00 $1.50
Colony Picking** each $0.50 $0.75

*Currently offered on demand — inquire by email

**Not currently offered

Billing and payment

Billing for our core and all OSRF cores is administrated by the OSRF Finance Team. We report services rendered to OSRF monthly. PIs and designated account administrators receive invoices from OSRF the month following order fulfillment. Invoices can be paid via the invoice payment template (UChicago users only), credit card or wire. The invoice payment template can be found here. For questions about payment not answered here, please reach out to the OSRF Finance Team at osrffinance@bsd.uchicago.edu.

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