Composite oral resections (including mandible, maxilla, palate, ear, temporal bone) require proper orientation and can be complex. CONSULT an attending and take gross photographs (BOTH intact and after sectioning).
Remember: For any tumor adjacent to / involving bone, if any part of the tumor is SOFT and does not NEED decalcification, please isolate 1-2 sections and submit as non-decalcified tumor. Then proceed with fixation and decalcification as necessary for the remainder of the case. Consider submitting a section in EDTA if the entire tissue requires decalcification (consult an attending).
Auto Text: “Insert Mandible”
Types of Mandibulectomies
Marginal Mandibulectomies consist of gingival mucosa, teeth, and the superficial portion of the mandible. The inferior mandibular cortex remains in the patient.
Segmental Mandibulectomies consist of a full-thickness segment of mandible, including gingival mucosa, teeth, bone, and often soft tissue/skeletal muscle adjacent to mandible.
- Orient specimen, noting laterality, anterior-posterior, superior-inferior, and medial-lateral.
- Photograph fresh specimen (prior to sectioning) in all planes that are helpful for visualization.
- Measure specimen in 3D (A-P, S-I, M-L). If applicable, separately measure bone versus attached large pieces of soft tissue / skin.
- Ink Margins: Think before you ink!
- Plan out your grossing strategy before inking. You can use different colors if it will help your later prosection (such as inner and outer sides different colors).
- Ink peripheral soft tissue margins.
- If a bone margin will be taken en face, it does not need to be inked. If a bone margin will be taken radially, ink the cut surface (looks like marrow surrounded by cortex).
- NOTE: The outer surface of thick bony cortex / subperiosteum does not usually need to be be inked, as it often rubs off and can be cumbersome.
- If specimen is small (such as marginal alveolar ridge resection), fix in formalin overnight. If large, you might want to section prior to fixation, to allow better penetration of formalin.
- Margins to consider include:
- Always discuss your approach to grossing with an attending.
- Specimens can usually be sectioned in one direction (perpendicular to longitudinal axis).
- Large specimens may be sectioned parallel to long axis into two halves. Then each half may be sectioned perpendicular to long axis.
- Photograph cut sections prior to decalcification.
- In order to facilitate decalcification, we recommend you cut your sections with a bone saw FIRST, and then decalcify thin sections. You can always thin down your sections after initial decalcification if they are too thick.
- For SMALL, DELICATE specimens, consider complete decalcification followed by sectioning with a long blade. ALWAYS consult an attending FIRST if this is your plan.
- Teeth will decalcify, but fillings or other foreign materials will NOT. If these materials are present, do NOT submit for histology.
- Always dictate which blocks were decalcified (or if entire specimen).