Hening Lin Research Group

Publications

  1. Hou D, Yu T, Lu X, Hong JY, Yang M, Zi Y, Ho TT, Lin H. Sirt2 inhibition improves gut epithelial barrier integrity and protects mice from colitis. Proc Natl Acad Sci U S A. 121:e2319833121, 2024. PMID: 38648480
  2. Peng K, Wallace SD, Bagde SR, Shang J, Anmangandla A, Jana S, Fromme JC, Lin H. GS-441524-Diphosphate-Ribose Derivatives as Nanomolar Binders and Fluorescence Polarization Tracers for SARS-CoV-2 and Other Viral Macrodomains. ACS Chem Biol. 2024 Apr 22. doi: 10.1021/acschembio.4c00027. Online ahead of print. PMID: 38646883
  3. Anmangandla A, Jana S, Peng K, Wallace SD, Bagde SR, Drown BS, Xu J, Hergenrother PJ, Fromme JC, Lin H. Correction to “A Fluorescence Polarization Assay for Macrodomains Facilitates the Identification of Potent Inhibitors of the SARS-CoV-2 Macrodomain”. ACS Chem Biol. 19:1023-1024, 2024. PMID: 38532633
  4. Tate EW, Soday L, de la Lastra AL, Wang M, Lin H. Protein lipidation in cancer: mechanisms, dysregulation and emerging drug targets. Nat Rev Cancer. 24:240-260, 2024 PMID: 38424304
  5. Zhang B, Yu Y, Fox BW, Liu Y, Thirumalaikumar VP, Skirycz A, Lin H, Schroeder FC. Amino acid and protein specificity of protein fatty acylation in C. elegans. Proc Natl Acad Sci U S A. 121:e2307515121, 2024. PMID: 38252833
  6. Peng K, Anmangandla A, Jana S, Jin Y, Lin H. Iso-ADP-Ribose Fluorescence Polarization Probe for the Screening of RNF146 WWE Domain Inhibitors. ACS Chem Biol. 19:300-307, 2024. PMID: 38237916
  7. Maio G, Smith M, Bhawal R, Zhang S, Baskin JM, Li J, Lin H. Interactome Analysis Identifies the Role of BZW2 in Promoting Endoplasmic Reticulum-Mitochondria Contact and Mitochondrial Metabolism. Mol Cell Proteomics. 23:100709, 2023 PMID: 38154691
  8. Tao Yu, Dan Hou, Jiaqi Zhao, Xuan Lu, Wendy K. Greentree, Qian Zhao, Min Yang, Don-Gerard Conde, Maurine E. Linder, Hening Lin. NLRP3 Cys126 palmitoylation by ZDHHC7 Promotes Inflammasome Activation, 43:114070, 2024 PMID 38583156
  9. Jiang Y, Xu Y, Zhu C, Xu G, Xu L, Rao Z, Zhou L, Jiang P, Malik S, Fang J, Lin H (co-corresponding), Zhang M. STAT3 palmitoylation initiates a positive feedback loop that promotes the malignancy of hepatocellular carcinoma cells in mice. Sci Signal. 16:eadd2282, 2023. PMID: 38051779
  10. Bai P, Liu Y, Yang L, Ding W, Mondal P, Sang N, Liu G, Lu X, Ho TT, Zhou Y, Wu R, Birar VC, Wilks MQ, Tanzi RE, Lin H, Zhang C, Li W, Shen S, Wang C. Development and Pharmacochemical Characterization Discover a Novel Brain-Permeable HDAC11-Selective Inhibitor with Therapeutic Potential by Regulating Neuroinflammation in Mice. J Med Chem. 2023 Nov 16. doi: 10.1021/acs.jmedchem.3c01491. Online ahead of print. PMID: 37972387
  11. Smith MR, Zhang L, Jin Y, Yang M, Bade A, Gillis KD, Jana S, Bypaneni RN, Glass TE, Lin H (co-corresponding). A Turn-On Fluorescent Amino Acid Sensor Reveals Chloroquine’s Effect on Cellular Amino Acids via Inhibiting Cathepsin L. ACS Cent Sci. 9(5):980-991, 2023. PMID: 37252359 Free PMC article.
  12. Zhang Y, Zhao Q, Lin H. Identification of potential HDAC11 deacylase substrates by affinity pulldown MS, Methods Enzymol. 685:43-55, 2023. PMID: 37245910
  13. Xu Y, Lin H. Use of alkyne-tagged myristic acid to detect N-terminal myristoylation. Methods Enzymol. 684:191-208, 2023. .PMID: 37230589
  14. Anmangandla A, Jana S, Peng K, Wallace SD, Bagde SR, Drown BS, Xu J, Hergenrother PJ, Fromme JC, Lin H. A Fluorescence Polarization Assay for Macrodomains Facilitates the Identification of Potent Inhibitors of the SARS-CoV-2 Macrodomain. ACS Chem Biol. doi: 10.1021/acschembio.3c00092. Online ahead of print. PMID: 37126856
  15. O’Brien C, Ling T, Berman JM, Culp-Hill R, Reisz JA, Rondeau V, Jahangiri S, St-Germain J, Macwan V, Astori A, Zeng A, Hong JY, Li M, Yang M, Jana S, Gamboni F, Tsao E, Liu W, Dick JE, Lin H, Melnick A, Tikhonova A, Arruda A, Minden MD, Raught B, D’Alessandro A, Jones CL. Simultaneous inhibition of Sirtuin 3 and cholesterol homeostasis targets acute myeloid leukemia stem cells by perturbing fatty acid β-oxidation and inducing lipotoxicity. 2023, doi: 10.3324/haematol.2022.281894. Online ahead of print. PMID: 37021547 Free article.
  16. Ho TT, Peng C, Seto E, Lin H. Trapoxin A Analogue as a Selective Nanomolar Inhibitor of HDAC11. ACS Chem Biol. 18:803-809, 2023. PMID: 36977486 Free PMC article.
  17. Wang ZA, Markert JW, Whedon SD, Yapa Abeywardana M, Lee K, Jiang H, Suarez C, Lin H, Farnung L, Cole PA. “Structural Basis of Sirtuin 6-Catalyzed Nucleosome Deacetylation”. J Am Chem Soc. Mar 17, 2023. Link
  18. Lin H. “Christopher T. Walsh: A Prolific Scientist, Effective Academic Leader, and Responsive Mentor”. ACS Chem Biol. Mar 15, 2023. Link
  19. Komaniecki G, Camarena MDC, Gelsleichter E, Mendoza R, Subler M, Windle JJ, Dozmorov MG, Lai Z, Sarkar D, Lin H. “Astrocyte Elevated Gene-1 Cys75 S-Palmitoylation by ZDHHC6 Regulates Its Biological Activity”. Biochemistry. Jan 17, 2023. Link
  20. Lin H. “Substrate-selective small-molecule modulators of enzymes: Mechanisms and opportunities”. Curr Opin Chem Biol. Feb, 2023. Link
  21. Latifkar A, Wang F, Mullmann JJ, Panizza E, Fernandez IR, Ling L, Miller AD, Fischbach C, Weiss RS, Lin H, Cerione RA, Antonyak MA. “IGF2BP2 promotes cancer progression by degrading the RNA transcript encoding a v-ATPase subunit”. Proc Natl Acad Sci U S A. Nov 8, 2022. Link
  22. Anmangandla A, Ren Y, Fu Q, Zhang S, Lin H. “The acyl-Coa specificity of human lysine acetyltransferase KAT2A” Biochemistry. Aug 22, 2022. Link
  23. Wang M, Zhang Y, Komaniecki GP, Lu X, Cao J, Zhang M, Yu T, Hou D, Spiegelman NA, Yang M, Price IR, Lin H. “Golgi stress induces SIRT2 to counteract Shigella infection via fatty de-acylation” Nat Commun. Aug 2, 2022. Link
  24. Miller SP, Maio G, Zhang X, Badillo Soto FS, Zhu J, Ramirez SZ, Lin H. “A proteomic approach identifies isoform-specific and nucleotide-dependent RAS interactions” Mol Cell Proteomics. July 12, 2022. Link
  25. Zhang Y, Su D, Zhu J, Wang M, Zhang Y, Fu Q, Zhang S, Lin H. “Oxygen level regulates N-terminal translation elongation of selected proteins through deoxyhypusine hydroxylation” Cell Rep. May 24, 2022. Link
  26. Zhang S, Nelson OD, Price IR, Zhu C, Lu X, Fernandez IR, Weiss RS, Lin H. “Long-chain fatty acyl coenzyme A inhibits NME1/2 and regulates cancer metastasis.” Proc Natl Acad Sci U S A. Mar 15, 2022. Link
  27. Wang ZA, Whedon SD, Wu M, Wang S, Brown EA, Anmangandla A, Regan L, Lee K, Du J, Hong JY, Fairall L, Kay T, Lin H, Zhao Y, Schwabe JWR, Cole PA. “Histone H2B Deacylation Selectivity: Exploring Chromatin’s Dark Matter with an Engineered Sortase.” J Am Chem Soc. Feb 17, 2022. Link
  28. Bagchi RA, Robinson EL, Hu T, Cao J, Hong JY, Tharp CA, Qasim H, Gavin KM, Pires da Silva J, Major JL, McConnell BK, Seto E, Lin H, McKinsey TA. “Reversible lysine fatty acylation of an anchoring protein mediates adipocyte adrenergic signaling.” Proc Natl Acad Sci U S A. Feb 15, 2022. Link
  29. Li M, Teater MR, Hong JY, Park NR, Duy C, Shen H, Wang L, Chen Z, Cerchietti L, Davidson SM, Lin H, Melnick AM. “Translational Activation of ATF4 through Mitochondrial Anaplerotic Metabolic Pathways Is Required for DLBCL Growth and Survival.” Blood Cancer Discov. Jan, 2022. Link
  30. Yang Y, Tapias V, Acosta D, Xu H, Chen H, Bhawal R, Anderson ET, Ivanova E, Lin H, Sagdullaev BT, Chen J, Klein WL, Viola KL, Gandy S, Haroutunian V, Beal MF, Eliezer D, Zhang S, Gibson GE.  “Altered succinylation of mitochondrial proteins, APP and tau in Alzheimer’s disease.” Nat Commun. Jan 10, 2022. Link
  31. Su D, Kosciuk T, Yang M, Price IR, Lin H. “Binding Affinity Determines Substrate Specificity and Enables Discovery of Substrates for N-Myristoyltransferases.” ACS Catal. Dec 17, 2021. Link
  32. Shang J, Smith MR, Anmangandla A, Lin H. “NAD+-consuming enzymes in immune defense against viral infection.” Biochem J. Dec 10, 2021. Link
  33. Cao Z, Gu Z, Lin S, Chen D, Wang J, Zhao Y, Li Y, Liu T, Li Y, Wang Y, Lin H, He B. “Attenuation of NLRP3 Inflammasome Activation by Indirubin-Derived PROTAC Targeting HDAC6.” ACS Chem Biol. Dec 3, 2021. Link
  34. Hong JY, Lin H. “Sirtuin Modulators in Cellular and Animal Models of Human Diseases.” Front Pharmacol. Oct 18, 2021. Link
  35. Cao Z, Yang F, Wang J, Gu Z, Lin S, Wang P, An J, Liu T, Li Y, Li Y, Lin H, Zhao Y, He B. “Indirubin Derivatives as Dual Inhibitors Targeting Cyclin-Dependent Kinase and Histone Deacetylase for Treating Cancer.” J Med Chem. Oct 8, 2021. Link
  36. Taneja A, Ravi V, Hong JY, Lin H, Sundaresan NR. “Emerging roles of Sirtuin 2 in cardiovascular diseases.” FASEB J. Oct 1, 2021. Link
  37. Zhang Y, Lin Z, Zhu J, Wang M, Lin H. “Diphthamide promotes TOR signaling by increasing the translation of proteins in the TORC1 pathway.” Proc Natl Acad Sci. Sep 14, 2021. Link
  38. Wang J, Cao Z, Wang F, Wang P, An J, Fu X, Liu T, Li Y, Li Y, Zhao Y, Lin H, He B. “Cysteine derivatives as acetyl lysine mimics to inhibit zinc-dependent histone deacetylases for treating cancer.” Eur J Med Chem. Aug 27, 2021. Link
  39. Hong JY, Cassel J, Yang J,Lin H, Weiser BP. “High-Throughput Screening Identifies Ascorbyl Palmitate As a SIRT2 Deacetylase and Defatty-Acylase Inhibitor.” ChemMedChem. Aug 12, 2021. Link
  40. Hong JY, Malgapo MIP, Liu Y, Yang M, Zhu C, Zhang X, Tolbert P, Linder ME, Lin H. “High-Throughput Enzyme Assay for Screening Inhibitors of the ZDHHC3/7/20 Acyltransferases.” ACS Chem Biol. Aug 10, 2021. Link
  41. Komaniecki G, Lin H. “Lysine Fatty Acylation: Regulatory Enzymes, Research Tools, and Biological Function.” Front Cell Dev Biol. Jul 22, 2021 Link
  42. Zhang Y, Su D, Dzikovski B, Majer SH, Coleman R, Chandrasekaran S, Fenwick MK, Crane BR, Lancaster KM, Freed JH, Lin H. “Dph3 Enables Aerobic Diphthamide Biosynthesis by Donating One Iron Atom to Transform a [3Fe-4S] to a [4Fe-4S] Cluster in Dph1-Dph2.” J Am Chem Soc. Jun 25, 2021 Link
  43. Hong JY, Fernandez I, Anmangandla A, Lu X, Bai JJ, Lin H. “Pharmacological Advantage of SIRT2-Selective versus pan-SIRT1-3 Inhibitors.” ACS Chem Biol. Jun 17, 2021 Link
  44. Wang M, Lin H. “Understanding the Function of Mammalian Sirtuins and Protein Lysine Acylation.” Annu Rev Biochem. Apr 13, 2021 Link
  45. Lin H. “Protein cysteine palmitoylation in immunity and inflammation.” FEBS J. Jan 27, 2021 Link
  46. Abril YLN, Fernandez IR, Hong JY, Chiang YL, Kutateladze DA, Zhao Q, Yang M, Hu J, Sadhukhan S, Li B, He B, Remick B, Bai JJ, Mullmann J, Wang F, Maymi V, Dhawan R, Auwerx J, Southard T, Cerione RA, Lin H, Weiss RS. “Pharmacological and genetic perturbation establish SIRT5 as a promising target in breast cancer.” Oncogene. Jan 21, 2021 Link
  47. Wielenberg K, Wang M, Yang M, Ozer A, Lis JT, Lin H. “An improved 4′-aminomethyltrioxsalen-based nucleic acid crosslinker for biotinylation of double-stranded DNA or RNA.” RSC Adv. Nov 2, 2020 Link
  48. Son SI, Su D, Ho TT, Lin H. “Garcinol is an HDAC11 Inhibitor” ACS Chem Biol. Oct 9, 2020 Link
  49. Zhang M, Zhou L, Xu Y, Yang M, Xu Y, Komaniecki GP, Kosciuk T, Chen X, Lu X, Zou Xiaoping, Linder ME, Lin H. “A STAT3 palmitoylation cycle promotes Th17 differentiation and colitis.” Nature. Oct 7, 2020 Link
  50. Hong JY, Jing H, Price IR, Cao J, Bai JJ, Lin H. “Simultaneous Inhibition of SIRT2 Deacetylase and Defatty-Acylase Activities via a PROTAC Strategy.” ACS Med Chem Letters. Sep 21, 2020 Link
  51. Bi D, Yang J, Hong JY, Parikh P, Hinds N, Infanti J, Lin H, Weiser BP. “Substrate-Dependent Modulation of SIRT2 by a Fluorescent Probe, 1-Aminoanthracene.” Biochemistry. Sep 17, 2020 Link
  52. Zhang L, Cao J, Dong L, Lin H.”TiPARP forms nuclear condensates to degrade HIF-1α and suppress tumorigenesis.” PNAS. Jun 1, 2020 Link
  53. Kosciuk T, Lin H. “NMT as a glycine and lysine myristoyltransferase in cancer, immunity, and infections.” ACS Chem Biol. May 26, 2020 Link
  54. Kosciuk T, Price IR, Zhang X, Zhu C, Johnson KN, Zhang S, Halaby SL, Komaniecki GP, Yang M, DeHart CJ, Thomas PM, Kelleher NL, Fromme JC, Lin H. “NMT1 and NMT2 are lysine myristoyltransferases regulating the ARF6 GTPase cycle.” Nat Commun. Feb 26, 2020 Link
  55. Fenwick MK, Su D, Dong M, Lin H, Ealick SE. “Structural Basis of the Substrate Selectivity of Viperin.” Biochemistry. Feb 11, 2020 Link
  56. Greene KS, Lukey MJ, Wang X, Blank B, Druso JE, Lin MJ, Stalnecker CA, Zhang C, Negrón Abril Y, Erickson JW, Wilson KF, Lin H, Weiss RS, Cerione RA. “SIRT5 stabilizes mitochondrial glutaminase and supports breast cancer tumorigenesis.” Proc Natl Acad Sci U S A. Dec 16, 2019 Link
  57. Zhang Y, Wang M, Lin H. “A Regulatory Cysteine Residue Mediates Reversible Inactivation of NAD+-Dependent Aldehyde Dehydrogenases to Promote Oxidative Stress Response.” ACS Chem Biol. Dec 16, 2019 Link
  58. Fenwick MK, Dong M, Lin H, Ealick SE. “Crystal structure of Dph2 in complex with elongation factor 2 reveals structural basis for first step of diphthamide biosynthesis.” Biochemistry. Sep 30, 2019 Link
  59. Dong M, Dando EE, Kotliar I, Su X, Dzikovski B, Freed JH, Lin H. “The asymmetric function of Dph1-Dph2 heterodimer in diphthamide biosynthesis.” J Biol Inorg Chem. Aug 28, 2019 Link
  60. Spiegelman NA, Zhang X, Jing H, Cao J, Kotliar IB, Aramsangtienchai P, Wang M, Tong Z, Rosch KM, Lin H. “SIRT2 and Lysine Fatty Acylation Regulate the Activity of RalB and Cell Migration.” ACS Chem Biol. Aug 21, 2019 Link
  61. Hong JY, Price IR, Bai JJ, Lin H. “A Glycoconjugated SIRT2 Inhibitor with Aqueous Solubility Allows Structure-Based Design of SIRT2 Inhibitors.” ACS Chem Biol. Aug 2, 2019 Link
  62. Son SI, Cao J, Zhu CL, Miller SP, Lin H. “Activity-Guided Design of HDAC11-Specific Inhibitors.” ACS Chem Biol. Jul 2, 2019 Link
  63. Li M, Chiang YL, Lyssiotis CA, Teater MR, Hong JY, Shen H, Wang L, Hu J, Jing H, Chen Z, Jain N, Duy C, Mistry SJ, Cerchietti L, Cross JR, Cantley LC, Green MR, Lin H, Melnick AM. “Non-oncogene Addiction to SIRT3 Plays a Critical Role in Lymphomagenesis.” Cancer Cell. Jun 10, 2019 Link
  64. Zhang S, Spiegelman NA, Lin H. “Global Profiling of Sirtuin Deacylase Substrates Using a Chemical Proteomic Strategy and Validation by Fluorescent Labeling.” Methods Mol Biol. Jun 4, 2019 Link
  65. Hong JY, Cao J, Lin H. “Fluorogenic Assays for the Defatty-Acylase Activity of Sirtuins.” Methods Mol Biol. Jun 4, 2019 Link
  66. Farooqi AS, Hong JY, Cao J, Lu X, Price IR, Zhao Q, Kosciuk T, Yang M, Bai JJ, Lin H. “Novel Lysine-Based Thioureas as Mechanism-Based Inhibitors of Sirtuin 2 (SIRT2) with Anticancer Activity in a Colorectal Cancer Murine Model.” J Med Chem. Apr 25, 2019 Link
  67. Latifkar A, Ling L, Hingorani A, Johansen E, Clement A, Zhang X, Hartman J, Fischbach C, Lin H, Cerione RA, Antonyak MA. “Loss of Sirtuin 1 Alters the Secretome of Breast Cancer Cells by Impairing Lysosomal Integrity.” Dev Cell. Apr 1, 2019 Link
  68. Wang H, Zeng X, Mo Y, He B, Lin H, Lin J. “Characterization of the enterobactin-specific antibodies induced by a novel enterobactin conjugate vaccine.” Appl Environ Microbiol. Mar 15, 2019 Link
  69. Kosciuk T, Wang M, Hong JY, Lin H. “Updates on the epigenetic roles of sirtuins.” Curr Opin Chem Biol. March 12, 2019 Link
  70. Cao J, Sun L, Aramsangtienchai P, Spiegelman NA, Zhang X, Huang W, Seto E, Lin H. “HDAC11 regulates type I interferon signaling through defatty-acylation of SHMT2.” PNAS. Feb 28, 2019 Link
  71. Spiegelman NA, Hong JY, Hu J, Jing H, Wang M, Price IR, Cao J, Yang M, Zhang X, Lin H. “A Small Molecule SIRT2 Inhibitor that Promotes K-Ras4a Lysine Fatty-acylation.” ChemMedChem. Feb 7, 2019 Link
  72. Wang WW, Angulo-Ibanez M, Lyu J, Kurra Y, Tong Z, Wu B, Zhang L, Sharma V, Zhou J, Lin H, Gao YQ, Li W, Chua KF, Liu WR. “A Click Chemistry Approach Reveals the Chromatin-Dependent Histone H3K36 Deacylase Nature of SIRT7.” J Am Chem Soc. Jan 17, 2019 Link
  73. Zhang Y, Lin Z, Wang M, Lin H. “Selective usage of isozymes for stress response.” ACS Chem Biol. Oct 17, 2018 Link
  74. Hong JY, Zhang X, Lin H. “HPLC-Based Enzyme Assays for Sirtuins.” Methods Mol Biol. August 11, 2018 Link
  75. Dong M, Zhang Y, Lin H. “Methods for Studying the Radical SAM Enzymes in Diphthamide Biosynthesis.” Methods Enzymol. 2018 Link
  76. Spiegelman NA, Price IR, Jing H, Wang M, Yang M, Cao J, Hong JY, Zhang X, Aramsangtienchai P, Sadhukhan S, Lin H. “Direct Comparison of SIRT2 Inhibitors: Potency, Specificity, Activity-Dependent Inhibition, and On-target Anticancer Activities.” ChemMedChem. July 29 2018 Link
  77. Dong M, Zhang Y, Lin H. “Noncanonical Radical SAM Enzyme Chemistry Learned from Diphthamide Biosynthesis.” Biochemistry. 2018 Link
  78. Dong M, Kathiresan V, Fenwick MK, Torelli AT, Zhang Y, Caranto JD, Dzikovski B, Sharma A, Lancaster K, Freed J, Ealick SE, Hoffman BM, Lin H. “Organometallic and radical intermediates reveal mechanism of diphthamide biosynthesis.” Science. March 16, 2018 Link
  79. Jiang H, Zhang X, Chen X, Aramsangtienchai P, Tong Z, Lin H. “Protein Lipidation: Occurrence, Mechanisms, Biological Functions, and Enabling Technologies.” Chem Rev. February 14, 2018 Link
  80. Zhang X, Cao J, Miller SP, Jing H, Lin H. “Comparative Nucleotide-Dependent Interactome Analysis Reveals Shared and Differential Properties of KRas4a and KRas4b.” ACS Cent Sci. January 24, 2018 Link
  81. Jing H, Zhang X, Wisner SA, Chen X, Spiegelman NA, Linder ME, Lin H. “SIRT2 and lysine fatty acylation regulate the transforming activity of K-Ras4a.” eLife. December 14, 2017 Link
  82. Yin X, Wu Q, Monga J, Xie E, Wang H, Wang S, Zhang H, Wang ZY, Zhou T, Shi Y, Rogers J, Lin H, Min J, Wang F. “HDAC1 Governs Iron Homeostasis Independent of Histone Deacetylation in Iron-Overload Murine Models.” Antioxidants & Redox Signaling. December 13, 2017 Link
  83. MacDonald RJ, Shrimp JH, Jiang H, Zhang L, Lin H, Yen A. “Probing the requirement for CD38 in retinoic acid-induced HL-60 cell differentiation with a small molecule dimerizer and genetic knockout.” Scientific Reports. December 12, 2017 Link
  84. Meng L, Chiang YL, Lyssiotis C, Teater M, Shen H, Wang L, Hong JY, Jin H, Duy C, Mistry C, Cerchietti L, Cross J, Cantley L, Lin H, Melnick A. “SIRT3 Is a Novel Metabolic Driver of and Therapeutic Target for Chemotherapy Resistant Dlbcls.” Blood. December 7, 2017 Link
  85. Zhang L, Lin H. “Using Clickable NAD+ Analogs to Label Substrate Proteins of PARPs.” Methods Mol Biol. July 11, 2017 Link
  86. Zhang X, Spiegelman NA, Nelson OD, Jing H, Lin H. “SIRT6 regulates Ras-related protein R-Ras2 by lysine defatty-acylation.” eLife. April 13, 2017 Link
  87. Dong M, Horitani M, Dzikovski B, Freed JH, Ealick SE, Hoffman BM, Lin H. “Substrate-Dependent Cleavage Site Selection by Unconventional Radical S-Adenosylmethionine Enzymes in Diphthamide Biosynthesis.” J Am Chem Soc. April 6, 2017 Link
  88. Wang Y, Fung YME, Zhang W, He B, Chung MWH, Jin J, Hu J, Lin H, Hao Q. “Deacylation mechanism by SIRT2 Revealed in the 1′-SH-2′-O-Myristoyl Intermediate Structure”, Cell Chem Biol. March 16, 2017 Link
  89. Aramsangtienchai P, Spiegelman NA, Cao J, Lin H. “S-Palmitoylation of Junctional Adhesion Molecule C Regulates Its Tight Junction Localization and Cell Migration.” J Biol Chem. Feb 14, 2017 Link
  90. Wang ZA, Kurra Y, Wang X, Zeng Y, Lee Y, Sharma V, Lin H, Dai SY, Liu WR. “A Versatile approach for site-specific lysine acetylation in proteins.” Angewandte Chemie. Jan 2, 2017 Link
  91. Tong Z, Wang M, Wang Y, Kim DD, Grenier JK, Cao J, Sadhukhan S, Hao Q, Lin H. “SIRT7 Is an RNA-Activated Protein Lysine Deacylase.” ACS Chem Biol. Nov 22, 2016 Link
  92. Lin Z, Dong M, Zhang Y, Lee EA, Lin H. “Cbr1 is a Dph3 reductase required for the tRNA wobble uridine modification”, Nat Chem Biol. Oct 3, 2016 Link
  93. Jin J, He B, Zhang X, Lin H, Wang Y. “SIRT2 Reverses 4-Oxonanoyl Lysine Modification on Histones”, J Am Chem Soc. 138, 12304-12307, 2016 Link
  94. Dong M, Horitani M, Dzikovski B, Pandelia M, Krebs C, Freed JH, Hoffman BM, Lin H. “Organometallic complex formed by an unconventional radical S-Adenosylmethionine Enzyme”, J Am Chem Soc. 138, 9755–9758, 2016 Link
  95. Sadhukhan S, Liu X, Ryu D, Nelson OD, Stupinski JA, Li Z, Chen W, Zhang S, Weiss RS, Locasale JW, Auwerx J, Lin H. “Metabolomics-assisted proteomics identifies succinylation and SIRT5 as important regulators of cardiac function.” PNAS. Apr 19, 2016 Link
  96. Aramsangtienchai P, Spiegelman NA, He B, Miller SP, Dai L, Zhao Y, Lin H. “HDAC8 catalyzes the hydrolysis of long chain fatty acyl lysine”, ACS Chem Biol. 2016 10.1021/acschembio.6b00396 Link
  97. Zhang X, Khan S, Jiang H, Antonyak MA, Chen X, Spiegelman NA, Shrimp JH, Cerione RA, Lin H. “Identifying the functional contribution of the defatty-acylase activity of SIRT6”, Nature Chemical Biology 12, 614–620, 2016 Link
  98. Tong Z, Wang Y, Zhang X, Kim DD, Sadhukhan S, Hao Q, Lin H. “SIRT7 Is Activated by DNA and Deacetylates Histone H3 in the Chromatin Context”, ACS Chem Biol. 11, 742-7, 2016 Link
  99. Jing H, Hu J, He B, Negrón Abril YL, Stupinski J, Weiser K, Carbonaro M, Chiang YL, Southard T, Giannakakou P, Weiss RS, Lin H. “A SIRT2-Selective Inhibitor Promotes c-Myc Oncoprotein Degradation and Exhibits Broad Anticancer Activity”, Cancer Cell. 29, 297-310, 2016 Link
  100. Chiang YL, Lin H. “An improved fluorogenic assay for SIRT1, SIRT2, and SIRT3”, Org Biomol Chem. 14, 2186-9, 2016 Link
  101. Li Z, Kim DD, Nelson OD, Otwell AE, Richardson RE, Callister SJ, Lin H. “Molecular dissection of a putative iron reductase from Desulfotomaculum reducens MI-1”, Biochem Biophys Res Commun 467, 503-8, 2015 Link
  102. Jing H, and Lin H. “Sirtuins in Epigenetic Regulation”, Chem. Rev., 115, 2350-2375, 2015 Link
  103. Liu X, Sadhukhan S, Sun S, Wagner GR, Hirschey MD, Qi L, Lin H, Locasale JW, “High resolution metabolomics with acyl-CoA profiling reveals widespread remodeling in response to diet.” Mol. Cell. Proteomics 2015, mcp.M114.044859 Link
  104. Teng YB, Jing H, Aramsangtienchai P, He B, Khan S, Hu J, Lin H, Hao Q, “Efficient demyristoylase activity of SIRT2 revealed by kinetic and structural studies.” Sci. Rep. 5, 8529, 2015. Link
  105. Otwell AE, Sherwood RW, Zhang S, Nelson OD, Li Z, Lin H, Callister SJ, Richardson RE, “Identification of proteins capable of metal reduction from the proteome of the Gram-positive bacterium Desulfotomaculum reducens MI-1 using an NADH-based activity assay.” Environ. Microbiol. 2014 Link
  106. He B, Hu J,   Zhang X, and Lin H. “Thiomyristoyl peptides as cell-permeable Sirt6 inhibitors”, Org. Biomol. Chem., 12, 7498-7502, 2014 Link
  107. Lin Z, Su X, Chen W, Ci B, Zhang S, Lin H. Dph7 catalyzes a previously unknown demethylation step in diphthamide biosynthesis. J Am Chem Soc. 2014 Link
  108. Shrimp JH, Hu J, Dong M, Wang BS, Macdonald RJ, Jiang H, Hao Q, Yen A, Lin H. Revealing CD38 Cellular Localization Using a Cell Permeable, Mechanism-Based Fluorescent Small Molecule Probe. J Am Chem Soc. 2014 Link
  109. Dong M, Su X, Dzikovski B, Dando EE, Zhu X, Du J, Freed JH, Lin H. Dph3 is an electron donor for dph1-dph2 in the first step of eukaryotic diphthamide biosynthesis. J Am Chem Soc. 136, 1754-7, 2014 Link
  110. G. Colak, Z. Xie, A. Zhu, L. Dai, Z. Lu, Y. Zhang, X. Wan, Y. Chen, Y.H. Cha, H. Lin, Y. Zhao, and M. Tan, “Identification of Lysine Succinylation Substrates and the Succinylation Regulatory Enzyme CobB in Escherichia coli”. Mol. Cell. Proteomics, 12, 3509-3520, 2013
  111. Yu J, Sadhukhan S, Noriega LG, Moullan N, He B, Weiss RS, Lin H, Schoonjans K, Auwerx J. “Metabolic Characterization of a Sirt5 deficient mouse model”, Sci. Rep. 3, 2806, 2013.
  112. Su X, Lin Z, Lin H. The biosynthesis and biological function of diphthamide. Crit Rev Biochem Mol Biol. 2013 Link
  113. Hu J, He B, Bhargava S, Lin H. A fluorogenic assay for screening Sirt6 modulators. Org Biomol Chem. 11, 5213-6, 2013 Link
  114. Jiang H, Khan S, Wang Y, Charron G, He B, Sebastian C, Du J, Kim R, Ge E, Mostoslavsky R, Hang HC, Hao Q, Lin H. SIRT6 regulates TNF-α secretion through hydrolysis of long-chain fatty acyl lysine. Nature. 496, 110-3. 2013 Link
  115. Jiang H, Lin H. Labeling Substrate Proteins of Poly(ADP-ribose) Polymerases with Clickable NAD Analog. Curr Protoc Chem Biol. 4, 19-34, 2012 Link
  116. Su X, Lin Z, Chen W, Jiang H, Zhang S, Lin H. Chemogenomic approach identified yeast YLR143W as diphthamide synthetase. Proc Natl Acad Sci U S A. 109, 19983-7, 2012 Link
  117. Jiang H, Sherwood R, Zhang S, Zhu X, Liu Q, Graeff R, Kriksunov IA, Lee HC, Hao Q, Lin H. Identification of ADP-ribosylation sites of CD38 mutants by precursor ion scanning mass spectrometry. Anal Biochem. 433, 218-26. 2012 Link
  118. Zhou Y, Zhang H, He B, Du J, Lin H, Cerione RA, Hao Q. The bicyclic intermediate structure provides insights into the desuccinylation mechanism of human sirtuin 5 (SIRT5). J Biol Chem. 287, 28307-14, 2012 Link
  119. Lin H, Su X, He B. Protein lysine acylation and cysteine succination by intermediates of energy metabolism, ACS Chem Biol. 7, 947-60, 2012 Link
  120. He B, Du J, Lin H. Thiosuccinyl peptides as Sirt5-specific inhibitors, J Am Chem Soc, 134, 1922-5, 2012 Link
  121. Su X, Chen W, Lee W, Jiang H, Zhang S, Lin H. YBR246W is required for the third step of diphthamide biosynthesis, J Am Chem Soc, 134(2), 773-6, 2012 Link
  122. Zhu AY, Zhou Y, Khan S, Deitsch KW, Hao Q, Lin H. Plasmodium falciparum Sir2A preferentially hydrolyzes medium and long chain fatty acyl lysine, ACS Chem Biol. 7, 155-9, 2012 Link
  123. J. Du, Y. Zhou, X. Su, J. Yu, S. Khan, H. Jiang, J. Kim, J. Woo, J. Kim, B. Choi, B. He, W. Chen, S. Zhang, R. Cerione, J. Auwerx, Q. Hao, and H.Lin. Sirt5 is a NAD-Dependent Protein Lysine Demalonylase and Desuccinylase, Science. Vol. 334, No 6507, 806-809, 2011 Link
  124. H. Lin, “S-Adenosylmethionine-dependent alkylation reactions: When are radical reactions used?” Bioorg. Chem. 39, 161-170, 2011
  125. X. Zhu, B. Dzikovski, X. Su, A.T. Torelli, Y. Zhang, S.E. Ealick, J.H. Freed, and H. Lin. Mechanistic understanding of Pyrococcus horikoshii Dph2, a [4Fe-4S] enzyme required for diphthamide biosynthesis, Mol. BioSystems. 7, 74-81, 2011 Link
  126. J. Congleton, H. Jiang, F. Malavasi, H. Lin, A. Yen, “ATRA-induced HL-60 myeloid leukemia cell differentiation depends on the CD38 cytosolic tail needed for membrane localization, but CD38 enzymatic activity is unnecessary” Exp. Cell Res. 317, 910-919 2011
  127. X. Zhu, J. Kim, X. Su, and H. Lin. Reconstitution of diphthine synthase activity in vitro, Biochemistry. 49, 9649-9657, 2010 . Link
  128. Hong Jiang, Jun Hyun Kim, Kristine M. Frizzell, W. Lee Kraus and Hening Lin;Clickable NAD Analogues for Labeling Substrate Proteins of Poly(ADP-ribose) Polymerases, JACS. 2010Link
  129. Yang Zhang, Xuling Zhu, Andrew T. Torelli, Michael Lee, Boris Dzikovski, Rachel M. Koralewski, Eileen Wang, Jack Freed, Carsten Krebs, Steven E. Ealick & Hening Lin;Diphthamide biosynthesis requires an organic radical generated by an iron–sulphur enzyme, Nature. 465,891-896, 2010 Link
  130. Q. Liu, R. Graeff, I. A. Kriksunov, H. Jiang, B. Zhang, B. V. L. Potter, N. Oppenheimer, H. Lin, H. C. Lee, Q. Hao, “Structural Basis for Enzymatic Evolution from a Dedicated ADP-ribosyl Cyclase to a Multi- functional NAD Hydrolase”, J. Biol. Chem., 284, 27637-27645, 2009. Link
  131. H. Jiang, J. Congleton, Q. Liu, P. Merchant, F. Malavasi, H.C. Lee, Q. Hao, A. Yen, H. Lin, “Mechanism-Based Small Molecule Probes for Labeling CD38 on Live Cells”, J. Am. Chem. Soc., 131, 1658-1659 (2009) Link Highlighted in Nat. Chem. Biol. 5, 149, 2009 Link
  132. J. Du, H. Jiang, H. Lin, “Investigating the ADP-ribosyltransferase activity of sirtuins with NAD analogs and 32P-NAD”, Biochemistry 48, 2878–2890, 2009 Link
  133. Q. Liu, I.A. Kriksunov, H. Jiang, R. Graeff, H. Lin, H.C. Lee, Q. Hao,“Covalent and non-covalent intermediates of an NAD utilizing enzyme – human CD38”, Chem. Biol. 15,1068-78, 2008 Link
  134. H. Lin, J. Du, H. Jiang, “Posttranslational modifications to regulate protein function”, Wiley Encyclopedia of Chemical Biology, 2008. John Wiley & Sons, Inc. DOI: 10.1002/9780470048672. wecb467 Link
  135. H. Lin, “Nicotinamide adenine dinucleotide: beyond a redox coenzyme”, Org. Biomol. Chem., 5, 2541-2554 2007 Link

 

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