Publications


Explore the full repertoire of discoveries from our lab: Schneewind and Missiakas on PubMed.

Staphylococcus

Stephanie Willing , Olaf Schneewind, Dominique Missiakas. Regulated cleavage of glycan strands by the murein hydrolase SagB in S. aureus involves a direct interaction with LyrA (SpdC).J Bacteriol.2021 Feb 16;JB.00014-21. doi: 10.1128/JB.00014-21

Dominique Missiakas , Volker Winstel. Selective Host Cell Death by Staphylococcus aureus: A Strategy for Bacterial Persistence. Front Immunol.2021 Jan21;11:621733. doi:10.3389/fimmu.2020.621733. eCollection 2020.

Manli Na , Zhicheng Hu , Majd Mohammad , Mariana do Nascimento Stroparo , Abukar Ali , Ying Fei , Anders Jarneborn , Peter Verhamme , Olaf Schneewind , Dominique Missiakas , Tao Jin. The Expression of von Willebrand Factor-Binding Protein Determines Joint-Invading Capacity of Staphylococcus aureus, a Core Mechanism of Septic Arthritis. mBio.2020 Nov 17;11(6):e02472-20. doi: 10.1128/mBio.02472-20.

Xinhai Chen, Miaomiao Shi, Xin Tong, Hwan Keun Kim, Lai-Xi Wang, Olaf Schneewind, and Dominique Missiakas. Glycosylation-dependent opsonophagocytic activity of staphylococcal protein A antibodies. PNAS September 15, 2020 117 (37) 22992-23000; first published August 27, 2020

Stephanie Willing, Emma Dyer, Olaf Schneewind and Dominique Missiakas. FmhA and FmhC of Staphylococcus aureus incorporate serine residues into peptidoglycan cross-bridges. doi: 10.1074/jbc.RA120.014371

Winstel V, Schneewind O, Missiakas D. Staphylococcus aureus Exploits the Host Apoptotic Pathway To Persist during Infection.
MBio. 2019 Nov 12;10(6). pii: e02270-19. doi: 10.1128/mBio.02270-19

Liesenborghs L, Meyers S, Lox M, Criel M, Claes J, Peetermans M, Trenson S, Vande Velde G, Vanden Berghe P, Baatsen P, Missiakas D, Schneewind O, Peetermans WE, Hoylaerts MF, Vanassche T, Verhamme P. Staphylococcus aureus endocarditis: distinct mechanisms of bacterial adhesion to damaged and inflamed heart valves.
Eur Heart J. 2019 Oct 14;40(39):3248-3259. doi: 10.1093/eurheartj/ehz175

Schneewind O, Missiakas DM. Staphylococcal Protein Secretion and Envelope Assembly.
Microbiol Spectr. 2019 Jul;7(4). doi: 10.1128/microbiolspec.GPP3-0070-2019

Putra I, Rabiee B, Anwar KN, Gidfar S, Shen X, Babalooee M, Ghassemi M, Afsharkhamseh N, Bakhsh S, Missiakas D, Nezamabadi A, Milani B, Eslani M, Djalilian AR. Staphylococcus aureus alpha-hemolysin impairs corneal epithelial wound healing and promotes intracellular bacterial invasion.
Exp Eye Res. 2019 Apr;181:263-270. doi: 10.1016/j.exer.2019.02.019. Epub 2019 Feb 27

Missiakas D. Staphylococcus aureus TarP: A Brick in the Wall or Rosetta Stone?
Cell Host Microbe. 2019 Feb 13;25(2):182-183. doi: 10.1016/j.chom.2019.01.013

Chen X, Sun Y, Missiakas D, Schneewind O. Staphylococcus aureus Decolonization of Mice With Monoclonal Antibody Neutralizing Protein A.
J Infect Dis. 2019 Feb 23;219(6):884-888. doi: 10.1093/infdis/jiy597

Schneewind O, Missiakas D. Sortases, Surface Proteins, and Their Roles in Staphylococcus aureus Disease and Vaccine Development.
Microbiol Spectr. 2019 Jan;7(1). doi: 10.1128/microbiolspec.PSIB-0004-2018

Bobrovskyy M, Willing SE, Schneewind O, Missiakas D. EssH Peptidoglycan Hydrolase Enables Staphylococcus aureus Type VII Secretion across the Bacterial Cell Wall Envelope.
J Bacteriol. 2018 Sep 24;200(20). pii: e00268-18. doi: 10.1128/JB.00268-18. Print 2018 Oct 15.

Ahmed MM, Aboshanab KM, Ragab YM, Missiakas DM, Aly KA. The transmembrane domain of the Staphylococcus aureus ESAT-6 component EssB mediates interaction with the integral membrane protein EsaA, facilitating partially regulated secretion in a heterologous host.
Arch Microbiol. 2018 Sep;200(7):1075-1086. doi: 10.1007/s00203-018-1519-x. Epub 2018 May 8

Winstel V, Missiakas D, Schneewind O. Staphylococcus aureus targets the purine salvage pathway to kill phagocytes.
Proc Natl Acad Sci U S A. 2018 Jun 26;115(26):6846-6851. doi: 10.1073/pnas.1805622115. Epub 2018 Jun 11

Yu W, Missiakas D, Schneewind O. Septal secretion of protein A in Staphylococcus aureus requires SecA and lipoteichoic acid synthesis.
Elife. 2018 May 14;7. pii: e34092. doi: 10.7554/eLife.34092

Sun Y, Emolo C, Holtfreter S, Wiles S, Kreiswirth B, Missiakas D, Schneewind O. Staphylococcal Protein A Contributes to Persistent Colonization of Mice with Staphylococcus aureus.
J Bacteriol. 2018 Apr 9;200(9). pii: e00735-17. doi: 10.1128/JB.00735-17. Print 2018 May 1.

Aly KA, Anderson M, Ohr RJ, Missiakas D. Isolation of a Membrane Protein Complex for Type VII Secretion in Staphylococcus aureus.
J Bacteriol. 2017 Oct 31;199(23). pii: e00482-17. doi: 10.1128/JB.00482-17. Print 2017 Dec 1.

Kant S, Asthana S, Missiakas D, Pancholi V. A novel STK1-targeted small-molecule as an “antibiotic resistance breaker” against multidrug-resistant Staphylococcus aureus.
Sci Rep. 2017 Jul 11;7(1):5067. doi: 10.1038/s41598-017-05314-z.

Claes J, Liesenborghs L, Peetermans M, Veloso TR, Missiakas D, Schneewind O, Mancini S, Entenza JM, Hoylaerts MF, Heying R, Verhamme P, Vanassche T. Clumping factor A, von Willebrand factor-binding protein and von Willebrand factor anchor Staphylococcus aureus to the vessel wall.
J Thromb Haemost. 2017 May;15(5):1009-1019. doi: 10.1111/jth.13653. Epub 2017 Mar 23

Missiakas D, Schneewind O. Staphylococcus aureus vaccines: Deviating from the carol.
J Exp Med. 2016 Aug 22;213(9):1645-53. doi: 10.1084/jem.20160569. Epub 2016 Aug 15. Review

Malachowa N, Kobayashi SD, Porter AR, Braughton KR, Scott DP, Gardner DJ, Missiakas DM, Schneewind O, DeLeo FR. Contribution of Staphylococcus aureus Coagulases and Clumping Factor A to Abscess Formation in a Rabbit Model of Skin and Soft Tissue Infection.
PLoS One. 2016 Jun 23;11(6):e0158293. doi: 10.1371/journal.pone.0158293. eCollection 2016.

Yu W, Kim HK, Rauch S, Schneewind O, Missiakas D. Pathogenic conversion of coagulase-negative staphylococci. Microbes Infect. 2017 Feb;19(2):101-109. doi: 10.1016/j.micinf.2016.12.002. Epub 2016 Dec 21.

Anderson M, Ohr RJ, Aly KA, Nocadello S, Kim HK, Schneewind CE, Schneewind O, Missiakas D. EssE Promotes Staphylococcus aureus ESS-Dependent Protein Secretion To Modify Host Immune Responses during Infection. J Bacteriol. 2016 Dec 13;199(1). pii: e00527-16. Print 2017 Jan 1.

Nygaard TK, Kobayashi SD, Freedman B, Porter AR, Voyich JM, Otto M, Schneewind O, DeLeo FR. Interaction of Staphylococci with Human B cells.
PLoS One. 2016 Oct 6;11(10):e0164410. doi: 10.1371/journal.pone.0164410. eCollection 2016

Ohr RJ, Anderson M, Shi M, Schneewind O, Missiakas D. EssD, a Nuclease Effector of the Staphylococcus aureus ESS Pathway. J Bacteriol. 2016 Dec 13;199(1). pii: e00528-16. Print 2017 Jan 1.

Schneewind O. Classic Spotlight: Molecular Biology of Methicillin Resistance in Staphylococcus aureus.
J Bacteriol. 2016 Jun 27;198(14):1903. doi: 10.1128/JB.00277-16. Print 2016 Jul 15

Thomer L, Schneewind O, Missiakas D. Pathogenesis of Staphylococcus aureus Bloodstream Infections. Annu Rev Pathol. 2016 May 23;11:343-64. doi: 10.1146/annurev-pathol-012615-044351. Epub 2016 Feb 25.

Kim HK, Falugi F, Missiakas DM, Schneewind O. Peptidoglycan-linked protein A promotes T cell-dependent antibody expansion during Staphylococcus aureus infection. Proc Natl Acad Sci U S A. 2016 May 17;113(20):5718-23. doi: 10.1073/pnas.1524267113. Epub 2016 May 2.

Thomer L, Emolo C, Thammavongsa V, Kim HK, McAdow ME, Yu W, Kieffer M, Schneewind O, Missiakas D. Antibodies against a secreted product of Staphylococcus aureus trigger phagocytic killing. J Exp Med. 2016 Mar 7;213(3):293-301. doi: 10.1084/jem.20150074. Epub 2016 Feb 15.

Chan YG, Frankel MB, Missiakas D, Schneewind O. SagB Glucosaminidase Is a Determinant of Staphylococcus aureus Glycan Chain Length, Antibiotic Susceptibility, and Protein Secretion. J Bacteriol. 2016 Jan 25;198(7):1123-36. doi: 10.1128/JB.00983-15.

Thammavongsa V, Kim HK, Missiakas D, Schneewind O. Staphylococcal manipulation of host immune responses. Nat Rev Microbiol. 2015 Sep;13(9):529-43. doi: 10.1038/nrmicro3521. Review.

Kim HK, Falugi F, Thomer L, Missiakas DM, Schneewind O. Protein A Suppresses Immune Responses during Staphylococcus aureus Bloodstream Infection in Guinea Pigs. MBio. 2015 Jan 6;6(1). pii: e02369-14.

Thammavongsa V, Rauch S, Kim HK, Missiakas DM, Schneewind O. Protein A-neutralizing monoclonal antibody protects neonatal mice against Staphylococcus aureus. Vaccine. 2015 Jan 15;33(4):523-6.

Pauli NT, Kim HK, Falugi F, Huang M, Dulac J, Henry Dunand C, Zheng NY, Kaur K, Andrews SF, Huang Y, DeDent A, Frank KM, Charnot-Katsikas A, Schneewind O, Wilson PC. Staphylococcus aureus infection induces protein A-mediated immune evasion in humans. J Exp Med. 2014 Nov 17;211(12):2331-9.

Rauch S, Gough P, Kim HK, Schneewind O, Missiakas D. Vaccine protection of leukopenic mice against Staphylococcus aureus bloodstream infection. Infect Immun. 2014 Nov;82(11):4889-98.

Kim HK, Missiakas D, Schneewind O. Mouse models for infectious diseases caused by Staphylococcus aureus. J Immunol Methods. 2014 Aug;410:88-99.

Chan YG, Kim HK, Schneewind O, Missiakas D. The capsular polysaccharide of Staphylococcus aureus is attached to peptidoglycan by the LytR-CpsA-Psr (LCP) family of enzymes. J Biol Chem. 2014 May 30;289(22):15680-90.

Becker S, Frankel MB, Schneewind O, Missiakas D. Release of protein A from the cell wall of Staphylococcus aureus. Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1574-9.

Cheng AG, Missiakas D, Schneewind O. The giant protein Ebh is a determinant of Staphylococcus aureus cell size and complement resistance. J Bacteriol. 2014 Mar;196(5):971-81.

Thomer L, Becker S, Emolo C, Quach A, Kim HK, Rauch S, Anderson M, Leblanc JF, Schneewind O, Faull KF, Missiakas D. N-acetylglucosaminylation of serine-aspartate repeat proteins promotes Staphylococcus aureus bloodstream infection. J Biol Chem. 2014 Feb 7;289(6):3478-86.

Kim HK, Emolo C, Missiakas D, Schneewind O. A monoclonal antibody that recognizes the E domain of staphylococcal protein A. Vaccine. 2014 Jan 16;32(4):464-9.

Thammavongsa V, Missiakas DM, Schneewind O. Staphylococcus aureus degrades neutrophil extracellular traps to promote immune cell death. Science. 2013 Nov 15;342(6160):863-6.

Anderson M, Aly KA, Chen YH, Missiakas D. Secretion of atypical protein substrates by the ESAT-6 secretion system of Staphylococcus aureus. Mol Microbiol. 2013 Nov;90(4):734-43.

Falugi F, Kim HK, Missiakas DM, Schneewind O. Role of protein A in the evasion of host adaptive immune responses by Staphylococcus aureus. MBio. 2013 Aug 27;4(5):e00575-13.

Chen C, Krishnan V, Macon K, Manne K, Narayana SV, Schneewind O. Secreted proteases control autolysin-mediated biofilm growth of Staphylococcus aureus. J Biol Chem. 2013 Oct 11;288(41):29440-52.

Thomer L, Schneewind O, Missiakas D. Multiple ligands of von Willebrand factor-binding protein (vWbp) promote Staphylococcus aureus clot formation in human plasma. J Biol Chem. 2013 Sep 27;288(39):28283-92.

Chan YG, Frankel MB, Dengler V, Schneewind O, Missiakas D. Staphylococcus aureus mutants lacking the LytR-CpsA-Psr family of enzymes release cell wall teichoic acids into the extracellular medium. J Bacteriol. 2013 Oct;195(20):4650-9.

Bacillus

Alice Chateau, So Young Oh, Anastasia Tomatsidou, Inka Brockhausen, Olaf Schneewind, Dominique Missiakas. Distinct pathways carry out α and β galactosylation of secondary cell wall polysaccharide in Bacillus anthracis. DOI: 10.1128/JB.00191-20

Chateau A, Lunderberg JM, Oh SY, Abshire T, Friedlander A, Quinn CP, Missiakas DM, Schneewind O. Galactosylation of the Secondary Cell Wall Polysaccharide of Bacillus anthracis and Its Contribution to Anthrax Pathogenesis.
J Bacteriol. 2018 Feb 7;200(5). pii: e00562-17. doi: 10.1128/JB.00562-17. Print 2018 Mar 1

Missiakas D, Schneewind O. Assembly and Function of the Bacillus anthracis S-Layer. Annu Rev Microbiol. 2017 Sep 8;71:79-98. doi: 10.1146/annurev-micro-090816-093512.

Callegan MC, Parkunan SM, Randall CB, Coburn PS, Miller FC, LaGrow AL, Astley RA, Land C, Oh SY, Schneewind O. The role of pili in Bacillus cereus intraocular infection.
Exp Eye Res. 2017 Jun;159:69-76. doi: 10.1016/j.exer.2017.03.007. Epub 2017 Mar 20

Oh SY, Lunderberg JM, Chateau A, Schneewind O, Missiakas D. Genes Required for Bacillus anthracis Secondary Cell Wall Polysaccharide Synthesis. J Bacteriol. 2016 Dec 13;199(1). pii: e00613-16. Print 2017 Jan 1.

Liszewski Zilla M, Lunderberg JM, Schneewind O, Missiakas D. Bacillus anthracis lcp Genes Support Vegetative Growth, Envelope Assembly, and Spore Formation. J Bacteriol. 2015 Dec 1;197(23):3731-41. doi: 10.1128/JB.00656-15.

Lunderberg JM, Liszewski Zilla M, Missiakas D, Schneewind O. Bacillus anthracis tagO Is Required for Vegetative Growth and Secondary Cell Wall Polysaccharide Synthesis. J Bacteriol. 2015 Nov;197(22):3511-20. doi: 10.1128/JB.00494-15.

Oh SY, Richter SG, Missiakas DM, Schneewind O. Glutamate Racemase Mutants of Bacillus anthracis. J Bacteriol. 2015 Jun;197(11):1854-61. doi: 10.1128/JB.00070-15.

Nguyen-Mau SM, Oh SY, Schneewind DI, Missiakas D, Schneewind O. Bacillus anthracis SlaQ Promotes S-Layer Protein Assembly. J Bacteriol. 2015 Oct;197(19):3216-27. doi: 10.1128/JB.00492-15.

Liszewski Zilla M, Chan YG, Lunderberg JM, Schneewind O, Missiakas D. LytR-CpsA-Psr Enzymes as Determinants of Bacillus anthracis Secondary Cell Wall Polysaccharide Assembly. J Bacteriol. 2015 Jan 15;197(2):343-53.

Wang YT, Missiakas D, Schneewind O. GneZ, a UDP-GlcNAc 2-epimerase, is required for S-layer assembly and vegetative growth of Bacillus anthracis. J Bacteriol. 2014 Aug 15;196(16):2969-78.

Oh SY, Maier H, Schroeder J, Richter GS, Elli D, Musser JM, Quenee LE, Missiakas DM, Schneewind O. Vaccine protection against Bacillus cereus-mediated respiratory anthrax-like disease in mice. Infect Immun. 2013 Mar;81(3):1008-17.

Lunderberg JM, Nguyen-Mau SM, Richter GS, Wang YT, Dworkin J, Missiakas DM, Schneewind O. Bacillus anthracis acetyltransferases PatA1 and PatA2 modify the secondary cell wall polysaccharide and affect the assembly of S-layer proteins. J Bacteriol. 2013 Mar;195(5):977-89.

Wang YT, Oh SY, Hendrickx AP, Lunderberg JM, Schneewind O. Bacillus cereus G9241 S-layer assembly contributes to the pathogenesis of anthrax-like disease in mice. J Bacteriol. 2013 Feb;195(3):596-605.

Yersinia

Osei-Owusu P, Charlton TM, Kim HK, Missiakas D, Schneewind O. FPR1 is the plague receptor on host immune cells.
Nature. 2019 Oct;574(7776):57-62. doi: 10.1038/s41586-019-1570-z. Epub 2019 Sep 18

Mitchell A, Tam C, Elli D, Charlton T, Osei-Owusu P, Fazlollahi F, Faull KF, Schneewind O. Glutathionylation of Yersinia pestis LcrV and Its Effects on Plague Pathogenesis.
MBio. 2017 May 16;8(3). pii: e00646-17. doi: 10.1128/mBio.00646-17

Schneewind O. Classic Spotlight: Studies on the Low-Calcium Response of Yersinia pestis Reveal the Secrets of Plague Pathogenesis.
J Bacteriol. 2016 Jul 13;198(15):2018. doi: 10.1128/JB.00358-16. Print 2016 Aug 1

Kopaskie KS, Ligtenberg KG, Schneewind O. Translational regulation of Yersinia enterocolitica mRNA encoding a type III secretion substrate. J Biol Chem. 2013 Dec 6;288(49):35478-88.

Ligtenberg KG, Miller NC, Mitchell A, Plano GV, Schneewind O. LcrV mutants that abolish Yersinia type III injectisome function. J Bacteriol. 2013 Feb;195(4):777-87.

Other

Kim HK, Premaratna R, Missiakas DM, Schneewind O. Rickettsia conorii O antigen is the target of bactericidal Weil-Felix antibodies.
Proc Natl Acad Sci U S A. 2019 Sep 24;116(39):19659-19664. doi: 10.1073/pnas.1911922116. Epub 2019 Aug 14

Bunker JJ, Drees C, Watson AR, Plunkett CH, Nagler CR, Schneewind O, Eren AM, Bendelac A. B cell superantigens in the human intestinal microbiota.
Sci Transl Med. 2019 Aug 28;11(507). pii: eaau9356. doi: 10.1126/scitranslmed.aau9356

Chateau A, Schneewind O, Missiakas D. Extraction and Purification of Wall-Bound Polymers of Gram-Positive Bacteria.
Methods Mol Biol. 2019;1954:47-57. doi: 10.1007/978-1-4939-9154-9_5

Mancini S, Oechslin F, Menzi C, Que YA, Claes J, Heying R, Veloso TR, Vanassche T, Missiakas D, Schneewind O, Moreillon P, Entenza JM. Marginal role of von Willebrand factor-binding protein and coagulase in the initiation of endocarditis in rats with catheter-induced aortic vegetations.
Virulence. 2018;9(1):1615-1624. doi: 10.1080/21505594.2018.1528845

Pigozzo AB, Missiakas D, Alonso S, Dos Santos RW, Lobosco M. Development of a Computational Model of Abscess Formation.
Front Microbiol. 2018 Jun 26;9:1355. doi: 10.3389/fmicb.2018.01355. eCollection 2018

Armitage JP, Becker A, Christie PJ, de Boer PAJ, DiRita VJ, Gourse RL, Henkin TM, Margolin W, Metcalf WW, Mullineaux CW, O’Toole GA, Parkinson JS, Schneewind O, Silhavy TJ, Stock AM, Zhulin IB. Classic Spotlights: Selected Highlights from the First 100 Years of the Journal of Bacteriology.
J Bacteriol. 2017 Jun 13;199(13). pii: e00062-17. doi: 10.1128/JB.00062-17. Print 2017 Jul 1

Schneewind O, Missiakas D. Lipoteichoic acids, phosphate-containing polymers in the envelope of gram-positive bacteria. J Bacteriol. 2014 Mar;196(6):1133-42.

Schneewind O, Missiakas D. Sec-secretion and sortase-mediated anchoring of proteins in Gram-positive bacteria. Biochim Biophys Acta. 2014 Aug;1843(8):1687-97.

Richter SG, Elli D, Kim HK, Hendrickx AP, Sorg JA, Schneewind O, Missiakas D. Small molecule inhibitor of lipoteichoic acid synthesis is an antibiotic for Gram-positive bacteria. Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3531-6.