2024
Wu, L.-F., Zhang, J., Cornwell-Arquitt, R., Hendrix, D.A., Radakovic, A., Szostak, J.W.*, 2024. Selective Nonenzymatic Formation of Biologically Common RNA Hairpins. Angew Chem Int Ed e202417370. PDF
Duzdevich, D.*, Carr, C.E., Colville, B.W.F., Aitken, H.R.M., Szostak, J.W., 2024. Overcoming nucleotide bias in the nonenzymatic copying of RNA templates. Nucleic Acids Research gkae982. PDF
Radakovic, A., Lewicka, A., Todisco, M., Aitken, H.R.M., Weiss, Z., Kim, S., Bannan, A., Piccirilli, J.A.*, Szostak, J.W.*, 2024. A potential role for RNA aminoacylation prior to its role in peptide synthesis. Proc. Natl. Acad. Sci. U.S.A. 121, e2410206121. PDF
Agrawal, A., Radakovic, A., Vonteddu, A., Rizvi, S., Huynh, V.N., Douglas, J.F., Tirrell, M.V.*, Karim, A.*, Szostak, J.W.*, 2024. Did the exposure of coacervate droplets to rain make them the first stable protocells? Sci. Adv. 10, eadn9657. PDF
Jia, X., Zhang, S.J., Zhou, L., Szostak, J.W.*, 2024. Constraints on the emergence of RNA through non-templated primer extension with mixtures of potentially prebiotic nucleotides. Nucleic Acids Research 52, 5451–5464. PDF
Jia, X., Fang, Z., Kim, S.C., Ding, D., Zhou, L., Szostak, J.W.*, 2024. Diaminopurine in Nonenzymatic RNA Template Copying. J. Am. Chem. Soc. 146, 15897–15907. PDF
Ravasio, R., Husain, K., Evans, C.G., Phillips, R., Ribezzi, M., Szostak, J.W.*, Murugan, A.*, 2024. A minimal scenario for the origin of non-equilibrium order. PDF
Mittal, S., Nisler, C., Szostak, J.W.*, 2024. Simulations predict preferred Mg2+ coordination in a nonenzymatic primer-extension reaction center. Biophysical Journal 123, 1579–1591. PDF
Todisco, M., Radakovic, A., Szostak, J.W.*, 2024. RNA Complexes with Nicks and Gaps: Thermodynamic and Kinetic Effects of Coaxial Stacking and Dangling Ends. J. Am. Chem. Soc. 146, 18083–18094. PDF
Fang, Z., Pazienza, L.T., Zhang, J., Tam, C.P., Szostak, J.W.*, 2024. Catalytic Metal Ion–Substrate Coordination during Nonenzymatic RNA Primer Extension. J. Am. Chem. Soc. 146, 10632–10639. PDF
Todisco, M., Ding, D., Szostak, J.W.*, 2024. Transient states during the annealing of mismatched and bulged oligonucleotides. Nucleic Acids Research 52, 2174–2187. PDF
Cohen, Z.R., Ding, D., Zhou, L., DasGupta, S., Haas, S., Sinclair, K.P., Todd, Z.R., Black, R.A., Szostak, J.W.*, Catling, D.C.*, 2024. Natural soda lakes provide compatible conditions for RNA and membrane function that could have enabled the origin of life. PNAS Nexus 3, pgae084. PDF
Ding, D., Fang, Z., Kim, S.C., O’Flaherty, D.K., Jia, X., Stone, T.B., Zhou, L.*, Szostak, J.W.*, 2024. Unusual Base Pair between Two 2-Thiouridines and Its Implication for Nonenzymatic RNA Copying. J. Am. Chem. Soc. 146, 3861–3871. PDF
DasGupta, S., Weiss, Z., Nisler, C., Szostak, J.W.*, 2024. Evolution of the substrate specificity of an RNA ligase ribozyme from phosphorimidazole- to triphosphate-activation (preprint) PDF
Radakovic, A., Lewicka, A., Todisco, M., Aitken, H.R.M., Weiss, Z., Kim, S., Bannan, A., Piccirilli, J.A., Szostak, J.W., 2024. Structure-guided aminoacylation and assembly of chimeric RNAs. (preprint) PDF
Kufner, C.L., Crucilla, S., Ding, D., Stadlbauer, P., Šponer, J., Szostak, J.W., Sasselov, D.D., Szabla, R.*, 2024. Photoinduced charge separation and DNA self-repair depend on sequence directionality and stacking pattern. Chem. Sci. 15, 2158–2166. PDF
Strom, K.R., Szostak, J.W.*, 2024. Sequence Information Transfer in Oligoarylacetylenes. ChemistryEurope 2, e202300073. PDF
2023
Toparlak, Ö.D., Sebastianelli, L., Egas Ortuno, V., Karki, M., Xing, Y., Szostak, J.W.*, Krishnamurthy, R.*, Mansy, S.S.*, 2023. Cyclophospholipids Enable a Protocellular Life Cycle. ACS Nano 17, 23772–23783. PDF
Fan, Z., Deckel, Y., Lowe, L.A., Loo, D.W.K., Yomo, T., Szostak, J.W., Nisler, C.*, Wang, A.*, 2023. Lipid Exchange Promotes Fusion of Model Protocells. Small Methods 7, 2300126. PDF
Lelyveld, V.S., Fang, Z., Szostak, J.W.*, 2023. Trivalent rare earth metal cofactors confer rapid NP-DNA polymerase activity. Science 382, 423–429. PDF
Ashwood, B., Jones, M.S., Radakovic, A., Khanna, S., Lee, Y., Sachleben, J.R., Szostak, J.W., Ferguson, A.L., Tokmakoff, A.*, 2023. Thermodynamics and kinetics of DNA and RNA dinucleotide hybridization to gaps and overhangs. Biophysical Journal 122, 3323–3339. PDF
DasGupta, S., Zhang, S., Szostak, J.W.*, 2023. Molecular Crowding Facilitates Ribozyme-Catalyzed RNA Assembly. ACS Cent. Sci. 9, 1670–1678. PDF
DasGupta, S., Zhang, S.J., Smela, M.P., Szostak, J.W.*, 2023. RNA‐Catalyzed RNA Ligation within Prebiotically Plausible Model Protocells. Chemistry A European J 29, e202301376. PDF
Ding, D., Zhang, S.J., Szostak, J.W.*, 2023. Enhanced nonenzymatic RNA copying with in-situ activation of short oligonucleotides. Nucleic Acids Research 51, 6528–6539. PDF
Aitken, H.R.M., Wright, T.H., Radakovic, A., Szostak, J.W.*, 2023. Small-Molecule Organocatalysis Facilitates In Situ Nucleotide Activation and RNA Copying. J. Am. Chem. Soc. 145, 16142–16149. PDF
Zhang, S.J., Lowe, L.A., Anees, P., Krishnan, Y., Fai, T.G., Szostak, J.W., Wang, A.*, 2023. Passive endocytosis in model protocells. Proc. Natl. Acad. Sci. U.S.A. 120, e2221064120. PDF
Ding, D., Zhou, L., Mittal, S., Szostak, J.W.*, 2023. Experimental Tests of the Virtual Circular Genome Model for Nonenzymatic RNA Replication. J. Am. Chem. Soc. 145, 7504–7515. PDF
Mittal, S., Nisler, C., Szostak, J.W.*, 2023. Simulations predict preferred Mg 2+ coordination in a nonenzymatic primer extension reaction center. PDF
Crucilla, S.J., Ding, D., Lozano, G.G., Szostak, J.W., Sasselov, D.D., Kufner, C.L.*, 2023. UV-driven self-repair of cyclobutane pyrimidine dimers in RNA. Chem. Commun. 59, 13603–13606. PDF
2022
Strom, K.R., Szostak, J.W.,* 2022. Folding and Duplex Formation in Sequence-Defined Aniline Benzaldehyde Oligoarylacetylenes. J. Am. Chem. Soc. 144, 18350–18358. PDF
Todisco, M., Szostak, J.W.*, 2022. Hybridization kinetics of out-of-equilibrium mixtures of short RNA oligonucleotides. Nucleic Acids Research 50, 9647–9662. PDF
Wu, L.-F., Liu, Z., Roberts, S.J., Su, M., Szostak, J.W.*, Sutherland, J.D.*, 2022. Template-Free Assembly of Functional RNAs by Loop-Closing Ligation. J. Am. Chem. Soc. 144, 13920–13927. PDF
Zhang, S.J., Duzdevich, D., Ding, D., Szostak, J.W.*, 2022. Freeze-thaw cycles enable a prebiotically plausible and continuous pathway from nucleotide activation to nonenzymatic RNA copying. Proc. Natl. Acad. Sci. U.S.A. 119, e2116429119. PDF
Ding, D., Zhou, L., Giurgiu, C., Szostak, J.W.*, 2022. Kinetic explanations for the sequence biases observed in the nonenzymatic copying of RNA templates. Nucleic Acids Research 50, 35–45. PDF
2021
Giurgiu, C., Fang, Z., Aitken, H.R.M., Kim, S.C., Pazienza, L., Mittal, S., Szostak, J.W.*, 2021. Structure–Activity Relationships in Nonenzymatic Template‐Directed RNA Synthesis. Angew Chem Int Ed 60, 22925–22932. PDF
Duzdevich, D., Carr, C.E., Ding, D., Zhang, S.J., Walton, T.S., Szostak, J.W.*, 2021. Competition between bridged dinucleotides and activated mononucleotides determines the error frequency of nonenzymatic RNA primer extension. Nucleic Acids Research 49, 3681–3691. PDF
Kim, S.C., O’Flaherty, D.K., Giurgiu, C., Zhou, L., Szostak, J.W.*, 2021. The Emergence of RNA from the Heterogeneous Products of Prebiotic Nucleotide Synthesis. J. Am. Chem. Soc. 143, 3267–3279. PDF
Radakovic, A., Wright, T.H., Lelyveld, V.S., Szostak, J.W.*, 2021. A Potential Role for Aminoacylation in Primordial RNA Copying Chemistry. Biochemistry 60, 477–488. PDF
Zhang, W., Kim, S.C., Tam, C.P., Lelyveld, V.S., Bala, S., Chaput, J.C., Szostak, J.W.*, 2021. Structural interpretation of the effects of threo-nucleotides on nonenzymatic template-directed polymerization. Nucleic Acids Research 49, 646–656. PDF
Zhou, L., Ding, D., Szostak, J.W.*, 2021. The virtual circular genome model for primordial RNA replication. RNA 27, 1–11. PDF
2020
Strom, K.R., Szostak, J.W.*, 2020. Solid-Phase Synthesis of Sequence-Defined Informational Oligomers. J. Org. Chem. 85, 13929–13938. PDF
Kindt, J.T., Szostak, J.W*., Wang, A.*, 2020. Bulk Self-Assembly of Giant, Unilamellar Vesicles. ACS Nano 14, 14627–14634. PDF
O’Flaherty, D.#, Zhou, L.#, Szostak, J.*, 2020. Nonenzymatic RNA-templated Synthesis of N3′→P5′ Phosphoramidate DNA. BIO-PROTOCOL 10. PDF
Zhou, L., O’Flaherty, D.K., Szostak, J.W.*, 2020. Assembly of a Ribozyme Ligase from Short Oligomers by Nonenzymatic Ligation. J. Am. Chem. Soc. 142, 15961–15965. PDF
Zhang, S.J., Duzdevich, D., Szostak, J.W.*, 2020. Potentially Prebiotic Activation Chemistry Compatible with Nonenzymatic RNA Copying. J. Am. Chem. Soc. 142, 14810–14813. PDF
Zhou, L., O’Flaherty, D.K., Szostak, J.W.*, 2020. Template‐Directed Copying of RNA by Non‐enzymatic Ligation. Angew Chem Int Ed 59, 15682–15687. PDF
Duzdevich, D., Carr, C.E., Szostak, J.W.*, 2020. Deep sequencing of non-enzymatic RNA primer extension. Nucleic Acids Research 48, e70–e70. PDF
Todd, Z.R.*, Fahrenbach, A.C., Ranjan, S., Magnani, C.J., Szostak, J.W., Sasselov, D.D., 2020. Ultraviolet-Driven Deamination of Cytidine Ribonucleotides Under Planetary Conditions. Astrobiology 20, 878–888. PDF
Yildirim, O., Izgu, E.C., Damle, M., Chalei, V., Ji, F., Sadreyev, R.I., Szostak, J.W., Kingston, R.E.*, 2020. S-phase Enriched Non-coding RNAs Regulate Gene Expression and Cell Cycle Progression. Cell Reports 31, 107629. PDF
Lelyveld, V.S., Zhang, W., Szostak, J.W.*, 2020. Synthesis of phosphoramidate-linked DNA by a modified DNA polymerase. Proc. Natl. Acad. Sci. U.S.A. 117, 7276–7283. PDF
Walton, T., DasGupta, S., Duzdevich, D., Oh, S.S., Szostak, J.W.*, 2020. In vitro selection of ribozyme ligases that use prebiotically plausible 2-aminoimidazole–activated substrates. Proc. Natl. Acad. Sci. U.S.A. 117, 5741–5748. PDF
Kim, S.C., Zhou, L., Zhang, W., O’Flaherty, D.K., Rondo-Brovetto, V., Szostak, J.W.*, 2020. A Model for the Emergence of RNA from a Prebiotically Plausible Mixture of Ribonucleotides, Arabinonucleotides, and 2′-Deoxynucleotides. J. Am. Chem. Soc. 142, 2317–2326. PDF
Chizzolini, F., Passalacqua, L.F.M., Oumais, M., Dingilian, A.I., Szostak, J.W.*, Lupták, A.*, 2020. Large Phenotypic Enhancement of Structured Random RNA Pools. J. Am. Chem. Soc. 142, 1941–1951. PDF
2019
Zhou, L., Kim, S.C., Ho, K.H., O’Flaherty, D.K., Giurgiu, C., Wright, T.H., Szostak, J.W.*, 2019. Non-enzymatic primer extension with strand displacement. eLife 8, e51888. PDF
Wang, A., Szostak, J.W.*, 2019. Lipid constituents of model protocell membranes. Emerging Topics in Life Sciences 3, 537–542. PDF
Wright, T.H., Giurgiu, C., Zhang, W., Radakovic, A., O’Flaherty, D.K., Zhou, L., Szostak, J.W.*, 2019. Prebiotically Plausible “Patching” of RNA Backbone Cleavage through a 3′–5′ Pyrophosphate Linkage. J. Am. Chem. Soc. 141, 18104–18112. PDF
Lelyveld, V.S., O’Flaherty, D.K., Zhou, L., Izgu, E.C., Szostak, J.W.*, 2019. DNA polymerase activity on synthetic N3′→P5′ phosphoramidate DNA templates. Nucleic Acids Research 47, 8941–8949. PDF
Todd, Z.R.*, Szabla, R., Szostak, J.W., Sasselov, D.D., 2019. UV photostability of three 2-aminoazoles with key roles in prebiotic chemistry on the early earth. Chem. Commun. 55, 10388–10391.PDF
Zhang, W., Pal, A., Ricardo, A., Szostak, J.W.*, 2019. Template-Directed Nonenzymatic Primer Extension Using 2-Methylimidazole-Activated Morpholino Derivatives of Guanosine and Cytidine. J. Am. Chem. Soc. 141, 12159–12166. PDF
O’Flaherty, D.K., Zhou, L., Szostak, J.W.*, 2019. Nonenzymatic Template-Directed Synthesis of Mixed-Sequence 3′-NP-DNA up to 25 Nucleotides Long Inside Model Protocells. J. Am. Chem. Soc. 141, 10481–10488. PDF
Walton, T., Zhang, W., Li, L., Tam, C.P., Szostak, J.W.*, 2019. The Mechanism of Nonenzymatic Template Copying with Imidazole‐Activated Nucleotides. Angew Chem Int Ed 58, 10812–10819. PDF
Strom, K.R.*, Szostak, J.W., Prywes, N.*, 2019. Transfer of Sequence Information and Replication of Diimine Duplexes. J. Org. Chem. 84, 3754–3761. PDF
Walton, T., Pazienza, L., Szostak, J.W.*, 2019. Template-Directed Catalysis of a Multistep Reaction Pathway for Nonenzymatic RNA Primer Extension. Biochemistry 58, 755–762. PDF
Matsushita-Ishiodori, Y., Hanczyc, M.M., Wang, A., Szostak, J.W., Yomo, T.*, 2019. Using Imaging Flow Cytometry to Quantify and Optimize Giant Vesicle Production by Water-in-oil Emulsion Transfer Methods. Langmuir 35, 2375–2382. PDF
Wang, A.*, Chan Miller, C., Szostak, J.W.*, 2019. Core-Shell Modeling of Light Scattering by Vesicles: Effect of Size, Contents, and Lamellarity. Biophysical Journal 116, 659–669. PDF
2018
Kim, S.C., O’Flaherty, D.K., Zhou, L., Lelyveld, V.S., Szostak, J.W.*, 2018. Inosine, but none of the 8-oxo-purines, is a plausible component of a primordial version of RNA. Proc. Natl. Acad. Sci. U.S.A. 115, 13318–13323. PDF
Jin, L., Engelhart, A.E., Zhang, W., Adamala, K., Szostak, J.W.*, 2018. Catalysis of Template-Directed Nonenzymatic RNA Copying by Iron(II). J. Am. Chem. Soc. 140, 15016–15021. PDF
Joyce, G.F.*, Szostak, J.W.*, 2018. Protocells and RNA Self-Replication. Cold Spring Harb Perspect Biol 10, a034801. PDF
Giurgiu, C., Wright, T.H., O’Flaherty, D.K., Szostak, J.W.,* 2018. A Fluorescent G‐Quadruplex Sensor for Chemical RNA Copying. Angew Chem Int Ed 57, 9844–9848. PDF
Zhang, W., Walton, T., Li, L., Szostak, J.W.*, 2018. Crystallographic observation of nonenzymatic RNA primer extension. eLife 7, e36422. PDF
O’Flaherty, D.K., Kamat, N.P.*, Mirza, F.N., Li, L., Prywes, N., Szostak, J.W.*, 2018. Copying of Mixed-Sequence RNA Templates inside Model Protocells. J. Am. Chem. Soc. 140, 5171–5178. PDF
Jin, L.#, Engelhart, A.E.#, Adamala, K.P., Szostak, J.W.*, 2018. Preparation, Purification, and Use of Fatty Acid-containing Liposomes. JoVE 57324. PDF
Jin, L., Kamat, N.P., Jena, S., Szostak, J.W.*, 2018. Fatty Acid/Phospholipid Blended Membranes: A Potential Intermediate State in Protocellular Evolution. Small 14, 1704077. PDF
Zhang, W., Tam, C.P., Zhou, L., Oh, S.S., Wang, J., Szostak, J.W., 2018. Structural Rationale for the Enhanced Catalysis of Nonenzymatic RNA Primer Extension by a Downstream Oligonucleotide. J. Am. Chem. Soc. 140, 2829–2840. PDF
Todd, Z.R.*, Fahrenbach, A.C., Magnani, C.J., Ranjan, S., Björkbom, A., Szostak, J.W., Sasselov, D.D., 2018. Solvated-electron production using cyanocuprates is compatible with the UV-environment on a Hadean–Archaean Earth. Chem. Commun. 54, 1121–1124. PDF
Tam, C.P., Zhou, L., Fahrenbach, A.C., Zhang, W., Walton, T., Szostak, J.W.*, 2018. Synthesis of a Nonhydrolyzable Nucleotide Phosphoroimidazolide Analogue That Catalyzes Nonenzymatic RNA Primer Extension. J. Am. Chem. Soc. 140, 783–792. PDF
2017
Giurgiu, C., Li, L., O’Flaherty, D.K., Tam, C.P., Szostak, J.W.*, 2017. A Mechanistic Explanation for the Regioselectivity of Nonenzymatic RNA Primer Extension. J. Am. Chem. Soc. 139, 16741–16747. PDF
Walton, T., Szostak, J.W.*, 2017. A Kinetic Model of Nonenzymatic RNA Polymerization by Cytidine-5′-phosphoro-2-aminoimidazolide. Biochemistry 56, 5739–5747. PDF
Ransey, E., Björkbom, A., Lelyveld, V.S., Biecek, P., Pantano, L., Szostak, J.W., Sliz, P.*, 2017. Comparative analysis of LIN28-RNA binding sites identified at single nucleotide resolution. RNA Biology 14, 1756–1765. PDF
Bonfio, C., Valer, L., Scintilla, S., Shah, S., Evans, D.J., Jin, L., Szostak, J.W., Sasselov, D.D., Sutherland, J.D., Mansy, S.S.*, 2017. UV-light-driven prebiotic synthesis of iron–sulfur clusters. Nature Chem 9, 1229–1234. PDF
Zhang, W., Tam, C.P., Walton, T., Fahrenbach, A.C., Birrane, G., Szostak, J.W.*, 2017. Insight into the mechanism of nonenzymatic RNA primer extension from the structure of an RNA-GpppG complex. Proc. Natl. Acad. Sci. U.S.A. 114, 7659–7664. PDF
Fahrenbach, A.C., Giurgiu, C., Tam, C.P., Li, L., Hongo, Y., Aono, M., Szostak, J.W.*, 2017. Common and Potentially Prebiotic Origin for Precursors of Nucleotide Synthesis and Activation. J. Am. Chem. Soc. 139, 8780–8783. PDF
Stairs, S., Nikmal, A., Bučar, D.-K., Zheng, S.-L., Szostak, J.W.*, Powner, M.W.*, 2017. Divergent prebiotic synthesis of pyrimidine and 8-oxo-purine ribonucleotides. Nat Commun 8, 15270. PDF
Szostak, J.W.*, 2017. The Narrow Road to the Deep Past: In Search of the Chemistry of the Origin of Life. Angew Chem Int Ed 56, 11037–11043. PDF
Li, L., Prywes, N., Tam, C.P., O’Flaherty, D.K., Lelyveld, V.S., Izgu, E.C., Pal, A., Szostak, J.W.*, 2017. Enhanced Nonenzymatic RNA Copying with 2-Aminoimidazole Activated Nucleotides. J. Am. Chem. Soc. 139, 1810–1813. PDF
Tam, C.P., Fahrenbach, A.C., Björkbom, A., Prywes, N., Izgu, E.C., Szostak, J.W.*, 2017. Downstream Oligonucleotides Strongly Enhance the Affinity of GMP to RNA Primer–Template Complexes. J. Am. Chem. Soc. 139, 571–574. PDF
2016
Zhang, W., Tam, C.P., Wang, J., Szostak, J.W.*, 2016. Unusual Base-Pairing Interactions in Monomer–Template Complexes. ACS Cent. Sci. 2, 916–926. PDF
Izgu, E.C., Björkbom, A., Kamat, N.P., Lelyveld, V.S., Zhang, W., Jia, T.Z., Szostak, J.W.*, 2016. N -Carboxyanhydride-Mediated Fatty Acylation of Amino Acids and Peptides for Functionalization of Protocell Membranes. J. Am. Chem. Soc. 138, 16669–16676. PDF
Pal, A., Das, R.S., Zhang, W., Lang, M., McLaughlin, L.W., Szostak, J.W.*, 2016. Effect of terminal 3′-hydroxymethyl modification of an RNA primer on nonenzymatic primer extension. Chem. Commun. 52, 11905–11907. PDF
Walton, T., Szostak, J.W.*, 2016. A Highly Reactive Imidazolium-Bridged Dinucleotide Intermediate in Nonenzymatic RNA Primer Extension. J. Am. Chem. Soc. 138, 11996–12002. PDF
Prywes, N., Blain, J.C., Del Frate, F., Szostak, J.W.*, 2016. Nonenzymatic copying of RNA templates containing all four letters is catalyzed by activated oligonucleotides. eLife 5, e17756. PDF
Prywes, N., Michaels, Y.S., Pal, A., Oh, S.S., Szostak, J.W.*, 2016. Thiolated uridine substrates and templates improve the rate and fidelity of ribozyme-catalyzed RNA copying. Chem. Commun. 52, 6529–6532. PDF
Engelhart, A.E., Adamala, K.P., Szostak, J.W.*, 2016. A simple physical mechanism enables homeostasis in primitive cells. Nature Chem 8, 448–453. PDF
Adamala, K.P., Engelhart, A.E., Szostak, J.W.*, 2016. Collaboration between primitive cell membranes and soluble catalysts. Nat Commun 7, 11041. PDF
Li, L., Lelyveld, V.S., Prywes, N., Szostak, J.W.*, 2016. Experimental and Computational Evidence for a Loose Transition State in Phosphoroimidazolide Hydrolysis. J. Am. Chem. Soc. 138, 3986–3989. PDF
Izgu, E.C., Oh, S.S., Szostak, J.W.*, 2016. Synthesis of activated 3′-amino-3′-deoxy-2-thio-thymidine, a superior substrate for the nonenzymatic copying of nucleic acid templates. Chem. Commun. 52, 3684–3686. PDF
2015
Lelyveld, V.S., Björkbom, A., Ransey, E.M., Sliz, P., Szostak, J.W.*, 2015. Pinpointing RNA-Protein Cross-Links with Site-Specific Stable Isotope-Labeled Oligonucleotides. J. Am. Chem. Soc. 137, 15378–15381. PDF
Björkbom, A., Lelyveld, V.S., Zhang, S., Zhang, W., Tam, C.P., Blain, J.C., Szostak, J.W.*, 2015. Bidirectional Direct Sequencing of Noncanonical RNA by Two-Dimensional Analysis of Mass Chromatograms. J. Am. Chem. Soc. 137, 14430–14438. PDF
Larsen, A.T., Fahrenbach, A.C., Sheng, J., Pian, J., Szostak, J.W.*, 2015. Thermodynamic insights into 2-thiouridine-enhanced RNA hybridization. Nucleic Acids Res 43, 7675–7687. PDF
Kamat, N.P., Tobé, S., Hill, I.T., Szostak, J.W.*, 2015. Electrostatic Localization of RNA to Protocell Membranes by Cationic Hydrophobic Peptides. Angew Chem Int Ed 54, 11735–11739. PDF
Adamala, K., Engelhart, A.E., Kamat, N.P., Jin, L., Szostak, J.W.*, 2015. Construction of a liposome dialyzer for the preparation of high-value, small-volume liposome formulations. Nat Protoc 10, 927–938. PDF
Izgu, E.C., Fahrenbach, A.C., Zhang, N., Li, L., Zhang, W., Larsen, A.T., Blain, J.C., Szostak, J.W.*, 2015. Uncovering the Thermodynamics of Monomer Binding for RNA Replication. J. Am. Chem. Soc. 137, 6373–6382. PDF
Heuberger, B.D., Pal, A., Del Frate, F., Topkar, V.V., Szostak, J.W.*, 2015. Replacing Uridine with 2-Thiouridine Enhances the Rate and Fidelity of Nonenzymatic RNA Primer Extension. J. Am. Chem. Soc. 137, 2769–2775. PDF
Adamala, K., Engelhart, A.E., Szostak, J.W.*, 2015. Generation of Functional RNAs from Inactive Oligonucleotide Complexes by Non-enzymatic Primer Extension. J. Am. Chem. Soc. 137, 483–489. PDF
2014
Hentrich, C., Szostak, J.W.*, 2014. Controlled Growth of Filamentous Fatty Acid Vesicles under Flow. Langmuir 30, 14916–14925. PDF
Budin, I., Prywes, N., Zhang, N., Szostak, J.W.*, 2014. Chain-Length Heterogeneity Allows for the Assembly of Fatty Acid Vesicles in Dilute Solutions. Biophysical Journal 107, 1582–1590. PDF
Sheng, J., Larsen, A., Heuberger, B.D., Blain, J.C., Szostak, J.W.*, 2014. Crystal Structure Studies of RNA Duplexes Containing s 2 U:A and s 2 U:U Base Pairs. J. Am. Chem. Soc. 136, 13916–13924. PDF
Blain, J.C., Szostak, J.W.*, 2014. Progress Toward Synthetic Cells. Annu. Rev. Biochem. 83, 615–640. PDF
Jia, T.Z., Hentrich, C., Szostak, J.W.*, 2014. Rapid RNA Exchange in Aqueous Two-Phase System and Coacervate Droplets. Orig Life Evol Biosph 44, 1–12. PDF
Sheng, J., Li, L., Engelhart, A.E., Gan, J., Wang, J., Szostak, J.W., 2014. Structural insights into the effects of 2′-5′ linkages on the RNA duplex. Proc. Natl. Acad. Sci. U.S.A. 111, 3050–3055. PDF
Li, L., Szostak, J.W., 2014. The Free Energy Landscape of Pseudorotation in 3′–5′ and 2′–5′ Linked Nucleic Acids. J. Am. Chem. Soc. 136, 2858–2865. PDF
Blain, J.C., Ricardo, A., Szostak, J.W., 2014. Synthesis and Nonenzymatic Template-Directed Polymerization of 2′-Amino-2′-deoxythreose Nucleotides. J. Am. Chem. Soc. 136, 2033–2039. PDF
2013
Adamala, K., Szostak, J.W., 2013. Nonenzymatic Template-Directed RNA Synthesis Inside Model Protocells. Science 342, 1098–1100. PDF
Zhu, T.F., Budin, I., Szostak, J.W., 2013. Preparation of Fatty Acid Micelles, in: Methods in Enzymology. Elsevier, pp. 283–288. PDF
Zhu, T.F., Budin, I., Szostak, J.W., 2013. Vesicle Extrusion Through Polycarbonate Track-etched Membranes using a Hand-held Mini-extruder, in: Methods in Enzymology. Elsevier, pp. 275–282. PDF
Zhu, T.F., Budin, I., Szostak, J.W., 2013. Preparation of Fatty Acid or Phospholipid Vesicles by Thin-film Rehydration, in: Methods in Enzymology. Elsevier, pp. 267–274. PDF
Josephson, K., Ricardo, A., Szostak, J.W., 2014. mRNA display: from basic principles to macrocycle drug discovery. Drug Discovery Today 19, 388–399. PDF
Zhang, S., Blain, J.C., Zielinska, D., Gryaznov, S.M., Szostak, J.W., 2013. Fast and accurate nonenzymatic copying of an RNA-like synthetic genetic polymer. Proc. Natl. Acad. Sci. U.S.A. 110, 17732–17737. PDF
Adamala, K., Szostak, J.W., 2013. Competition between model protocells driven by an encapsulated catalyst. Nature Chem 5, 495–501. PDF
Engelhart, A.E., Powner, M.W., Szostak, J.W., 2013. Functional RNAs exhibit tolerance for non-heritable 2′–5′ versus 3′–5′ backbone heterogeneity. Nature Chem 5, 390–394. PDF
Zhang, S., Zhang, N., Blain, J.C., Szostak, J.W., 2013. Synthesis of N3′-P5′-linked Phosphoramidate DNA by Nonenzymatic Template-Directed Primer Extension. J. Am. Chem. Soc. 135, 924–932. PDF
2012
Budin, I., Debnath, A., Szostak, J.W., 2012. Concentration-Driven Growth of Model Protocell Membranes. J. Am. Chem. Soc. 134, 20812–20819. PDF
Powner, M.W.*, Zheng, S.-L., Szostak, J.W., 2012. Multicomponent Assembly of Proposed DNA Precursors in Water. J. Am. Chem. Soc. 134, 13889–13895. PDF
Szostak, J.W., 2012. The eightfold path to non-enzymatic RNA replication. J Syst Chem 3, 2. PDF
Zhu, T.F., Adamala, K., Zhang, N., Szostak, J.W., 2012. Photochemically driven redox chemistry induces protocell membrane pearling and division. Proc. Natl. Acad. Sci. U.S.A. 109, 9828–9832. PDF
Hofmann, F.T., Szostak, J.W., Seebeck, F.P., 2012. In Vitro Selection of Functional Lantipeptides. J. Am. Chem. Soc. 134, 8038–8041. PDF
Guillen Schlippe, Y.V., Hartman, M.C.T., Josephson, K., Szostak, J.W., 2012. In Vitro Selection of Highly Modified Cyclic Peptides That Act as Tight Binding Inhibitors. J. Am. Chem. Soc. 134, 10469–10477. PDF
Zhang, N., Zhang, S., Szostak, J.W., 2012. Activated Ribonucleotides Undergo a Sugar Pucker Switch upon Binding to a Single-Stranded RNA Template. J. Am. Chem. Soc. 134, 3691–3694. PDF
Szostak, J.W., 2012. Attempts to Define Life Do Not Help to Understand the Origin of Life. Journal of Biomolecular Structure and Dynamics 29, 599–600. PDF
2011
Szostak, J.W., 2011. An optimal degree of physical and chemical heterogeneity for the origin of life? Phil. Trans. R. Soc. B 366, 2894–2901. PDF
Seebeck, F.P., Ricardo, A., Szostak, J.W., 2011. Artificial lantipeptides from in vitro translations. Chem. Commun. 47, 6141. PDF
Zhu, T.F., Szostak, J.W., 2011. Exploding vesicles. J Syst Chem 2, 4. PDF
Trevino, S.G., Zhang, N., Elenko, M.P., Lupták, A., Szostak, J.W., 2011. Evolution of functional nucleic acids in the presence of nonheritable backbone heterogeneity. Proc. Natl. Acad. Sci. U.S.A. 108, 13492–13497. PDF
Budin, I., Szostak, J.W., 2011. Physical effects underlying the transition from primitive to modern cell membranes. Proc. Natl. Acad. Sci. U.S.A. 108, 5249–5254. PDF
Subtelny, A.O., Hartman, M.C.T., Szostak, J.W., 2011. Optimal Codon Choice Can Improve the Efficiency and Fidelity of N ‐Methyl Amino Acid Incorporation into Peptides by In‐Vitro Translation. Angew Chem Int Ed 50, 3164–3167. PDF
2010
Powner, M.W., Sutherland, J.D., Szostak, J.W., 2011. Chemoselective Multicomponent One-Pot Assembly of Purine Precursors in Water. J. Am. Chem. Soc. 133, 4149–4150. PDF
Elenko, M.P., Szostak, J.W., Van Oijen, A.M., 2010. Single-molecule binding experiments on long time scales. Review of Scientific Instruments 81, 083705. PDF
Schrum, J.P., Zhu, T.F., Szostak, J.W., 2010. The origins of cellular life. Cold Spring Harb Perspect Biol 2, a002212. PDF
Rajamani, S., Ichida, J.K., Antal, T., Treco, D.A., Leu, K., Nowak, M.A., Szostak, J.W., Chen, I.A., 2010. Effect of Stalling after Mismatches on the Error Catastrophe in Nonenzymatic Nucleic Acid Replication. J. Am. Chem. Soc. 132, 5880–5885. PDF
Budin, I., Szostak, J.W., 2010. Expanding Roles for Diverse Physical Phenomena During the Origin of Life. Annu. Rev. Biophys. 39, 245–263. PDF
2009
Bruckner, R.J., Mansy, S.S., Ricardo, A., Mahadevan, L., Szostak, J.W., 2009. Flip-Flop-Induced Relaxation of Bending Energy: Implications for Membrane Remodeling. Biophysical Journal 97, 3113–3122. PDF
Schrum, J.P., Ricardo, A., Krishnamurthy, M., Blain, J.C., Szostak, J.W., 2009. Efficient and Rapid Template-Directed Nucleic Acid Copying Using 2′-Amino-2′,3′-dideoxyribonucleoside−5′-Phosphorimidazolide Monomers. J. Am. Chem. Soc. 131, 14560–14570. PDF
Mansy, S.S., Szostak, J.W., 2009. Reconstructing the Emergence of Cellular Life through the Synthesis of Model Protocells. Cold Spring Harbor Symposia on Quantitative Biology 74, 47–54. PDF
Lin, S., Horning, D.P., Szostak, J.W., Chaput, J.C., 2009. Conformational Analysis of DNA Repair Intermediates by Time-Resolved Fluorescence Spectroscopy. J. Phys. Chem. A 113, 9585–9587. PDF
Budin, I., Bruckner, R.J., Szostak, J.W., 2009. Formation of Protocell-like Vesicles in a Thermal Diffusion Column. J. Am. Chem. Soc. 131, 9628–9629. PDF
Elenko, M.P., Szostak, J.W., Van Oijen, A.M., 2009. Single-Molecule Imaging of an in Vitro -Evolved RNA Aptamer Reveals Homogeneous Ligand Binding Kinetics. J. Am. Chem. Soc. 131, 9866–9867. PDF
Szostak, J.W., 2009. Systems chemistry on early Earth. Nature 459, 171–172. PDF
Zhu, T.F., Szostak, J.W., 2009. Preparation of Large Monodisperse Vesicles. PLoS ONE 4, e5009. PDF
Zhu, T.F., Szostak, J.W., 2009. Coupled Growth and Division of Model Protocell Membranes. J. Am. Chem. Soc. 131, 5705–5713. PDF
Chen, J.J., Tsai, C.-H., Cai, X., Horhota, A.T., McLaughlin, L.W., Szostak, J.W., 2009. Enzymatic Primer-Extension with Glycerol-Nucleoside Triphosphates on DNA Templates. PLoS ONE 4, e4949. PDF
Chen, J.J., Cai, X., Szostak, J.W., 2009. N2′→P3′ Phosphoramidate Glycerol Nucleic Acid as a Potential Alternative Genetic System. J. Am. Chem. Soc. 131, 2119–2121. PDF
2008
Mansy, S.S., Szostak, J.W., 2008. Thermostability of model protocell membranes. Proc. Natl. Acad. Sci. U.S.A. 105, 13351–13355. PDF
Mansy, S.S., Schrum, J.P., Krishnamurthy, M., Tobé, S., Treco, D.A., Szostak, J.W., 2008. Template-directed synthesis of a genetic polymer in a model protocell. Nature 454, 122–125. PDF
Subtelny, A.O., Hartman, M.C.T., Szostak, J.W., 2008. Ribosomal Synthesis of N -Methyl Peptides. J. Am. Chem. Soc. 130, 6131–6136. PDF
Monnard, P.-A., Szostak, J.W., 2008. Metal-ion catalyzed polymerization in the eutectic phase in water–ice: A possible approach to template-directed RNA polymerization. Journal of Inorganic Biochemistry 102, 1104–1111. PDF
2007
Hartman, M.C.T., Josephson, K., Lin, C.-W., Szostak, J.W., 2007. An Expanded Set of Amino Acid Analogs for the Ribosomal Translation of Unnatural Peptides. PLoS ONE 2, e972. PDF
Tsai, C.-H., Chen, J., Szostak, J.W., 2007. Enzymatic synthesis of DNA on glycerol nucleic acid templates without stable duplex formation between product and template. Proc. Natl. Acad. Sci. U.S.A. 104, 14598–14603. PDF
Seelig, B., Szostak, J.W., 2007. Selection and evolution of enzymes from a partially randomized non-catalytic scaffold. Nature 448, 828–831. PDF
Mansy, S.S., Zhang, J., Kümmerle, R., Nilsson, M., Chou, J.J., Szostak, J.W., Chaput, J.C., 2007. Structure and Evolutionary Analysis of a Non-biological ATP-binding Protein. Journal of Molecular Biology 371, 501–513. PDF
Smith, M.D., Rosenow, M.A., Wang, M., Allen, J.P., Szostak, J.W., Chaput, J.C., 2007. Structural Insights into the Evolution of a Non-Biological Protein: Importance of Surface Residues in Protein Fold Optimization. PLoS ONE 2, e467. PDF
Hazen, R.M., Griffin, P.L., Carothers, J.M., Szostak, J.W., 2007. Functional information and the emergence of biocomplexity. Proc. Natl. Acad. Sci. U.S.A. 104, 8574–8581. PDF
Meena, Sam, M., Pierce, K., Szostak, J.W., McLaughlin, L.W., 2007. 2‘,3‘-Dideoxy-3‘-thionucleoside Triphosphates: Syntheses and Polymerase Substrate Activities. Org. Lett. 9, 1161–1163. PDF
2006
Horhota, A.T., Szostak, J.W., McLaughlin, L.W., 2006. Glycerol Nucleoside Triphosphates: Synthesis and Polymerase Substrate Activities. Org. Lett. 8, 5345–5347. PDF
Blackburn, E.H., Greider, C.W., Szostak, J.W., 2006. Telomeres and telomerase: the path from maize, Tetrahymena and yeast to human cancer and aging. Nat Med 12, 1133–1138. PDF
Salehi-Ashtiani, K., Lupták, A., Litovchick, A., Szostak, J.W., 2006. A Genomewide Search for Ribozymes Reveals an HDV-Like Sequence in the Human CPEB3 Gene. Science 313, 1788–1792. PDF
Hanczyc, M.M., Mansy, S.S., Szostak, J.W., 2007. Mineral Surface Directed Membrane Assembly. Orig Life Evol Biosph 37, 67–82. PDF
Carothers, J.M., Oestreich, S.C., Szostak, J.W., 2006. Aptamers Selected for Higher-Affinity Binding Are Not More Specific for the Target Ligand. J. Am. Chem. Soc. 128, 7929–7937. PDF
Seebeck, F.P., Szostak, J.W., 2006. Ribosomal Synthesis of Dehydroalanine-Containing Peptides. J. Am. Chem. Soc. 128, 7150–7151. PDF
Hartman, M.C.T., Josephson, K., Szostak, J.W., 2006. Enzymatic aminoacylation of tRNA with unnatural amino acids. Proc. Natl. Acad. Sci. U.S.A. 103, 4356–4361. PDF
Carothers, J.M., Davis, J.H., Chou, J.J., Szostak, J.W., 2006. Solution structure of an informationally complex high-affinity RNA aptamer to GTP. RNA 12, 567–579. PDF
Cho, G.S., Szostak, J.W., 2006. Directed Evolution of ATP Binding Proteins from a Zinc Finger Domain by Using mRNA Display. Chemistry & Biology 13, 139–147. PDF
2005
Fujikawa, S.M., Chen, I.A., Szostak, J.W., 2005. Shrink-Wrap Vesicles. Langmuir 21, 12124–12129. PDF
Plummer, K.A., 2005. In vitro selection of RNA aptamers against a composite small molecule-protein surface. Nucleic Acids Research 33, 5602–5610. PDF
Chen, I.A., Salehi-Ashtiani, K., Szostak, J.W., 2005. RNA Catalysis in Model Protocell Vesicles. J. Am. Chem. Soc. 127, 13213–13219. PDF
Ichida, J.K., 2005. High fidelity TNA synthesis by Therminator polymerase. Nucleic Acids Research 33, 5219–5225. PDF
Josephson, K., Hartman, M.C.T., Szostak, J.W., 2005. Ribosomal Synthesis of Unnatural Peptides. J. Am. Chem. Soc. 127, 11727–11735. PDF
Horhota, A., Zou, K., Ichida, J.K., Yu, B., McLaughlin, L.W., Szostak, J.W., Chaput, J.C., 2005. Kinetic Analysis of an Efficient DNA-Dependent TNA Polymerase. J. Am. Chem. Soc. 127, 7427–7434. PDF
Shen, X., Valencia, C.A., Szostak, J., Dong, B., Liu, R., 2005. Scanning the human proteome for calmodulin-binding proteins. Proc. Natl. Acad. Sci. U.S.A. 102, 5969–5974. PDF correction
Sacerdote, M.G., Szostak, J.W., 2005. Semipermeable lipid bilayers exhibit diastereoselectivity favoring ribose. Proc. Natl. Acad. Sci. U.S.A. 102, 6004–6008. PDF
Zou, K., Horhota, A., Yu, B., Szostak, J.W., McLaughlin, L.W., 2005. Synthesis of α- l -Threofuranosyl Nucleoside Triphosphates (tNTPs). Org. Lett. 7, 1485–1487. PDF
Ichida, J.K., Zou, K., Horhota, A., Yu, B., McLaughlin, L.W., Szostak, J.W., 2005. An in Vitro Selection System for TNA. J. Am. Chem. Soc. 127, 2802–2803. PDF
2004
Hanczyc, M.M., Szostak, J.W., 2004. Replicating vesicles as models of primitive cell growth and division. Current Opinion in Chemical Biology 8, 660–664. PDF
Chen, I.A., Roberts, R.W., Szostak, J.W., 2004. The Emergence of Competition Between Model Protocells. Science 305, 1474–1476. PDF
Chen, I.A., Szostak, J.W., 2004. A Kinetic Study of the Growth of Fatty Acid Vesicles. Biophysical Journal 87, 988–998. PDF
Sazani, P.L., Larralde, R., Szostak, J.W., 2004. A Small Aptamer with Strong and Specific Recognition of the Triphosphate of ATP. J. Am. Chem. Soc. 126, 8370–8371. PDF
Chaput, J.C., Szostak, J.W., 2004. Evolutionary Optimization of a Nonbiological ATP Binding Protein for Improved Folding Stability. Chemistry & Biology 11, 865–874. PDF
Chen, I.A., Szostak, J.W., 2004. Membrane growth can generate a transmembrane pH gradient in fatty acid vesicles. Proc. Natl. Acad. Sci. U.S.A. 101, 7965–7970. PDF
Carothers, J.M., Oestreich, S.C., Davis, J.H., Szostak, J.W., 2004. Informational Complexity and Functional Activity of RNA Structures. J. Am. Chem. Soc. 126, 5130–5137. PDF
2003
Hanczyc, M.M., Fujikawa, S.M., Szostak, J.W., 2003. Experimental Models of Primitive Cellular Compartments: Encapsulation, Growth, and Division. Science 302, 618–622. PDF
Chaput, J.C., Szostak, J.W., 2003. TNA Synthesis by DNA Polymerases. J. Am. Chem. Soc. 125, 9274–9275. PDF
Vaish, N.K., Larralde, R., Fraley, A.W., Szostak, J.W., McLaughlin, L.W., 2003. A Novel, Modification-Dependent ATP-Binding Aptamer Selected from an RNA Library Incorporating a Cationic Functionality. Biochemistry 42, 8842–8851. PDF
Szostak, J.W., 2003. Functional information: Molecular messages. Nature 423, 689–689. PDF
Huang, Z., Szostak, J.W., 2003. Selective labeling and detection of specific RNAs in an RNA mixture. Analytical Biochemistry 315, 129–133. PDF
Chaput, J.C., Ichida, J.K., Szostak, J.W., 2003. DNA Polymerase-Mediated DNA Synthesis on a TNA Template. J. Am. Chem. Soc. 125, 856–857. PDF
2002
Davis, J.H., Szostak, J.W., 2002. Isolation of high-affinity GTP aptamers from partially structured RNA libraries. Proc. Natl. Acad. Sci. U.S.A. 99, 11616–11621. PDF
Baggio, R., Burgstaller, P., Hale, S.P., Putney, A.R., Lane, M., Lipovsek, D., Wright, M.C., Roberts, R.W., Liu, R., Szostak, J.W., Wagner, R.W., 2002. Identification of epitope‐like consensus motifs using mRNA display. J of Molecular Recognition 15, 126–134. PDF
2001
Salehi-Ashtiani, K., Szostak, J.W., 2001. In vitro evolution suggests multiple origins for the hammerhead ribozyme. Nature 414, 82–84. PDF
Szostak, J.W., Bartel, D.P., Luisi, P.L., 2001. Synthesizing life. Nature 409, 387–390. PDF
Keefe, A.D., Szostak, J.W., 2001. Functional proteins from a random-sequence library. Nature 410, 715–718. PDF
Keefe, A.D., Wilson, D.S., Seelig, B., Szostak, J.W., 2001. One-Step Purification of Recombinant Proteins Using a Nanomolar-Affinity Streptavidin-Binding Peptide, the SBP-Tag. Protein Expression and Purification 23, 440–446. PDF
Wilson, D.S., Keefe, A.D., Szostak, J.W., 2001. The use of mRNA display to select high-affinity protein-binding peptides. Proc. Natl. Acad. Sci. U.S.A. 98, 3750–3755. PDF
2000
Cho, G., Keefe, A.D., Liu, R., Wilson, D.S., Szostak, J.W., 2000. Constructing high complexity synthetic libraries of long ORFs using In Vitro selection. Journal of Molecular Biology 297, 309–319. PDF
Vaish, N.K., 2000. Expanding the structural and functional diversity of RNA: analog uridine triphosphates as candidates for in vitro selection of nucleic acids. Nucleic Acids Research 28, 3316–3322. PDF
Suga, H., Lee, N., Bessho, Y., Wei, K., Szostak, J.W., 2000. Ribozyme-catalyzed tRNA aminoacylation. Nat. Struct Biol. 7, 28–33. PDF
Liu, R., Barrick, J.E., Szostak, J.W., Roberts, R.W., 2000. [19] Optimized synthesis of RNA-protein fusions for in vitro protein selection, in: Methods in Enzymology. Elsevier, pp. 268–293. PDF
1999
Wilson, D.S., Szostak, J.W., 1999. In Vitro Selection of Functional Nucleic Acids. Annu. Rev. Biochem. 68, 611–647. PDF
1998
Samuelson, J.C. (Ed.), 2013. Enzyme engineering: methods and protocols, Methods in molecular biology. Humana Press, New York, N.Y. Heidelberg. PDF
Holeman, L.A., Robinson, S.L., Szostak, J.W., Wilson, C., 1998. Isolation and characterization of fluorophore-binding RNA aptamers. Folding and Design 3, 423–431. PDF
Wilson, C., Szostak, J.W., 1998. Isolation of a fluorophore-specific DNA aptamer with weak redox activity. Chemistry & Biology 5, 609–617. PDF
Wilson, C., Nix, J., Szostak, J., 1998. Functional Requirements for Specific Ligand Recognition by a Biotin-Binding RNA Pseudoknot. Biochemistry 37, 14410–14419. PDF
Suga, H., Cowan, J.A., Szostak, J.W., 1998. Unusual Metal Ion Catalysis in an Acyl-Transferase Ribozyme. Biochemistry 37, 10118–10125. PDF
Suga, H., Lohse, P.A., Szostak, J.W., 1998. Structural and Kinetic Characterization of an Acyl Transferase Ribozyme. J. Am. Chem. Soc. 120, 1151–1156. PDF
1997
Roberts, R.W., Szostak, J.W., 1997. RNA-peptide fusions for the in vitro selection of peptides and proteins. Proc. Natl. Acad. Sci. U.S.A. 94, 12297–12302. PDF
Hager, A.J., Szostak, J.W., 1997. Isolation of novel ribozymes that ligate AMP-activated RNA substrates. Chemistry & Biology 4, 607–617. PDF
Dieckmann, T., Butcher, S.E., Sassanfar, M., Szostak, J.W., Feigon, J., 1997. Mutant ATP-binding RNA aptamers reveal the structural basis for ligand binding. Journal of Molecular Biology 273, 467–478. PDF
Szostak, J.W., 1997. Introduction: Combinatorial Chemistry. Chem. Rev. 97, 347–348. PDF
1996
Hager, A.J., Pollard, J.D., Szostak, J.W., 1996. Ribozymes: aiming at RNA replication and protein synthesis. Chemistry & Biology 3, 717–725. PDF
Huang, Z., 1996. A simple method for 3’-labeling of RNA. Nucleic Acids Research 24, 4360–4361. PDF
Lohse, P.A., Szostak, J.W., 1996. Ribozyme-catalysed amino-acid transfer reactions. Nature 381, 442–444. PDF
Rohatgi, R., Bartel, D.P., Szostak, J.W., 1996. Nonenzymatic, Template-Directed Ligation of Oligoribonucleotides Is Highly Regioselective for the Formation of 3‘−5‘ Phosphodiester Bonds. J. Am. Chem. Soc. 118, 3340–3344. PDF
Rohatgi, R., Bartel, D.P., Szostak, J.W., 1996. Kinetic and Mechanistic Analysis of Nonenzymatic, Template-Directed Oligoribonucleotide Ligation. J. Am. Chem. Soc. 118, 3332–3339. PDF
Lorsch, J.R., Szostak, J.W., 1996. Chance and Necessity in the Selection of Nucleic Acid Catalysts. Acc. Chem. Res. 29, 103–110. PDF
Wilson, D.S., Szostak, J.W., 1999. In Vitro Selection of Functional Nucleic Acids. Annu. Rev. Biochem. 68, 611–647. PDF
1995
Lorsch, J.R., Bartel, D.P., Szostak, J.W., 1995. Reverse transcriptase reads through a 2′–5′ linkage and a 2′-thiphosphate in a template. Nucl Acids Res 23, 2811–2814. PDF
Ekland, E.H., Szostak, J.W., Bartel, D.P., 1995. Structurally Complex and Highly Active RNA Ligases Derived from Random RNA Sequences. Science 269, 364–370. PDF
Cuenoud, B., Szostak, J.W., 1995. A DNA metalloenzyme with DNA ligase activity. Nature 375, 611–614. PDF
Chapman, K.B., Szostak, J.W., 1995. Isolation of a ribozyme with 5′-5′ ligase activity. Chemistry & Biology 2, 325–333. PDF
Wilson, C., Szostak, J.W., 1995. In vitro evolution of a self-alkylatlng ribozyme. Nature 374, 777–782. PDF
Whoriskey, S.K., Usman, N., Szostak, J.W., 1995. Total chemical synthesis of a ribozyme derived from a group I intron. Proc. Natl. Acad. Sci. U.S.A. 92, 2465–2469. PDF
Lauhon, C.T., Szostak, J.W., 1995. RNA aptamers that bind flavin and nicotinamide redox cofactors. J. Am. Chem. Soc. 117, 1246–1257. PDF
Huizenga, D.E., Szostak, J.W., 1995. A DNA Aptamer That Binds Adenosine and ATP. Biochemistry 34, 656–665. PDF
1994
Hanna, M., Szostak, J.W., 1994. Suppression of mutations in the core of the Tetrahymena ribozyme by spermidine, ethanol and by substrate stabilization. Nucl Acids Res 22, 5326–5331. PDF
Lorsch, J.R., Szostak, J.W., 1994. In vitro evolution of new ribozymes with polynucleotide kinase activity. Nature 371, 31–36. PDF
Chapman, K.B., Szostak, J.W., 1994. In vitro selection of catalytic RNAs. Current Opinion in Structural Biology 4, 618–622. PDF
Peterson, R.D., Bartel, D.P., Szostak, J.W., Horvath, S.J., Feigon, J., 1994. 1H NMR studies of the high-affinity Rev binding site of the Rev responsive element of HIV-1 mRNA: base pairing in the core binding element. Biochemistry 33, 5357–5366. PDF
Lorsch, J.R., Szostak, J.W., 1994. In vitro selection of RNA aptamers specific for cyanocobalamin. Biochemistry 33, 973–982. PDF
Lin, C.W., Hanna, M., Szostak, J.W., 1994. Evidence That the Guanosine Substrate of the Tetrahymena Ribozyme Is Bound in the Anti Conformation and That N7 Contributes to Binding. Biochemistry 33, 2703–2707. PDF
Green, R., Szostak, J.W., 1994. In vitro genetic analysis of the hinge region between helical elements P5-P4-P6 and P7-P3-P8 in the sunY group I self-splicing intron. Journal of Molecular Biology 235, 140–155. PDF
1993
Bartel, D.P., Szostak, J.W., 1993. Isolation of New Ribozymes from a Large Pool of Random Sequences. Science 261, 1411–1418. PDF
Sassanfar, M., Szostak, J.W., 1993. An RNA motif that binds ATP. Nature 364, 550–553. PDF
Doudna, J.A., Usman, N., Szostak, J.W., 1993. Ribozyme-catalyzed primer extension by trinucleotides: A model for the RNA-catalyzed replication of RNA. Biochemistry 32, 2111–2115. PDF
1992
Green, R., Szostak, J.W., 1992. Selection of a Ribozyme That Functions as a Superior Template in a Self-Copying Reaction. Science 258, 1910–1915. PDF
Famulok, M., Szostak, J.W., 1992. In Vitro Selection of Specific Ligand‐binding Nucleic Acids. Angew. Chem. Int. Ed. Engl. 31, 979–988. PDF
Famulok, M., Szostak, J.W., 1992. Stereospecific recognition of tryptophan agarose by in vitro selected RNA. J. Am. Chem. Soc. 114, 3990–3991. PDF
Szostak, J.W., 1992. In vitro genetics. Trends in Biochemical Sciences 17, 89–93. PDF
Ellington, A.D., Szostak, J.W., 1992. Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures. Nature 355, 850–852. PDF
1991
Green, R., Szostak, J.W., Benner, S.A., Rich, A., Usman, N., 1991. Synthesis of RNA containing inosine: analysis of the sequence requirements for the 5′ splice site of the Tetrahymena group I intron. Nucl Acids Res 19, 4161–4166. PDF
Bartel, D.P., Zapp, M.L., Green, M.R., Szostak, J.W., 1991. HIV-1 rev regulation involves recognition of non-Watson-Crick base pairs in viral RNA. Cell 67, 529–536. PDF
Green, R., Ellington, A., Bartel, D., Szostak, J., 1991. In vitro genetic analysis: Selection and amplification of rare functional nucleic acids. Methods 2, 75–86. PDF
Bartel, D.P., Doudna, J.A., Usman, N., Szostak, J.W., 1991. Template-directed primer extension catalyzed by the Tetrahymena ribozyme. Mol. Cell. Biol. 11, 3390–3394. PDF
Doudna, J.A., Couture, S., Szostak, J.W., 1991. A Multisubunit Ribozyme That Is a Catalyst of and Template for Complementary Strand RNA synthesis. Science 251, 1605–1608. PDF
1990
Doudna, J.A., Szostak, J.W., Rich, A., Usman, N., 1990. Chemical synthesis of oligoribonucleotides containing 2-aminopurine: substrates for the investigation of ribozyme function. J. Org. Chem. 55, 5547–5549. PDF
Michel, F., Ellington, A.D., Couture, S., Szostak, J.W., 1990. Phylogenetic and genetic evidence for base-triples in the catalytic domain of group I introns. Nature 347, 578–580. PDF
Ellington, A.D., Szostak, J.W., 1990. In vitro selection of RNA molecules that bind specific ligands. Nature 346, 818–822. PDF
1987
Doudna, J.A., Gerber, A.S., Cherry, J.M., Szostak, J.W., 1987. Genetic Dissection of an RNA Enzyme. Cold Spring Harbor Symposia on Quantitative Biology 52, 173–180. PDF