Tang Lab @ UChicago

Publications

Independent Career @ UChicago

 8. Yan H, Tan E, Zou S, Sun Y, Ke A*, Tang W*, Assessing and engineering the IscB-ωRNA system for programmed genome editing. Nat. Chem. Biol. 2024, https://doi.org/10.1038/s41589-024-01669-3.

 7. Yan H, Tang W*, Programmed RNA Editing with an Evolved Bacterial Adenosine Deaminase. Nat. Chem. Biol. 2024, https://doi.org/10.1038/s41589-024-01661-x.

6. Ge R, He ME, Tang W*, N6-Methyladenosine in Mammalian Messenger RNA: Function, Location, and Quantitation. Isr. J. Chem. 2024, https://doi.org/10.1002/ijch.202300181.

 5. Xiao Y#, Chen Y-M#, Zou Z, Ye C, Dou X, Wu J, Liu C, Liu S, Yan H, Wang P, Zeng T-B, Liu Q, Fei J, Tang W, He C*, Profiling of RNA-Binding Protein Binding Sites by In Situ Reverse Transcription-Based Sequencing. Nat. Methods. 2024, https://doi.org/10.1038/s41592-023-02146-w.

4. Xiao YL, Wu Y, Tang W*, An Adenine Base Editor Variant Expands Context Compatibility. Nat. Biotechnol. 2024, https://doi.org/10.1038/s41587-023-01994-3.

Addgene link for E. coli and mammalian expression plasmids: https://www.addgene.org/browse/article/28238713/

Research Highlight: Tang L, More Deaminases Towards Precise Editing. Nat. Methods. 2024, https://doi.org/10.1038/s41592-024-02193-x.

3. Wu T#, Liu C#, Zou S#, Lyu R, Yang B, Yan H, Zhao M*, Tang W*, An Engineered Hypercompact CRISPR-Cas12f System with Boosted Gene-Editing Activity. Nat. Chem. Biol. 2023, https://doi.org/10.1038/s41589-023-01380-9.

Addgene link for E. coli and mammalian expression plasmids: https://www.addgene.org/browse/article/28238262/

2. Huang AT, Tang W*, Smoking-Related DNA Alkylation Events Are Mapped at Single-Nucleotide Resolution. News & Views. ACS Cent. Sci. 2023, 9, 346-348, https://doi.org/10.1021/acscentsci.3c00246.

1. Xiao YL#, Liu S#, Ge R#, Wu Y, He C,* Chen M,* Tang W,* Transcriptome-wide Profiling and Quantification of N6-methyladenosine by Enzyme-Assisted Adenosine Deamination, Nat. Biotechnol. 2023, https://doi.org/10.1038/s41587-022-01587-6.

Addgene link for E. coli expression plasmid of TadA 8.20: https://www.addgene.org/194702/

Post-doc Research @ Broad Institute

  1. Tang W, Liu DR*. Rewritable multi-event analog recording in bacterial and mammalian cells. Science 2018, 360, eaap8992
  2. Hu JH, Miller SM, Geurts MH, Tang W, Chen L, Sun N, Zeina C, Gao X, Rees HA, Lin Z, Liu DR*. Evolved Cas9 variants with broad PAM compatibility and high DNA specificity. Nature 2018, 556, 57–63
  3. Tang W, Hu JH, Liu DR*. Aptazyme-embedded guide RNAs enable ligand-responsive genome engineering. Nat. Commun. 2017, 8, 15939

Ph.D. Research @ Illinois

  1. Rahman IR, Sanchez A, Tang W, van der Donk WA*. Structure-activity relationships of the Enterococcal cytolysin. ACS Infect. Dis. 2021, 7, 2445-2454.
  2. Bobeica SC, Zhu L, Acedo JZ, Tang W, van der Donk WA*. Structural determinants of macrocyclization in substrate-controlled lanthipeptide biosynthetic pathways. Chem. Sci. 2020, 11, 12854-12870.
  3. Tang W, Bobeica SC, Wang L, van der Donk WA*. CylA is a sequence-specific protease involved in toxin biosynthesis. J. Ind. Microbiol. Biotechnol. 2019, 46, 537-549
  4. Tang W, Thibodeaux GN, van der Donk WA*. The enterococcal cytolysin synthetase coevolves with substrate for stereoselective lanthionine synthesis. ACS Chem. Biol. 2016, 11, 2438-2446
  5. Dong SH#, Tang W#, Lukk T, Nair SK*, van der Donk WA*. The enterococcal cytolysin synthetase has an unanticipated lipid kinase fold. eLife 2015, 4, 07607 (# equal contribution)
  6. Tang W, Jiménez-Osés G, Houk KN*, van der Donk WA*. Substrate control in stereoselective lanthionine biosynthesis. Nat. Chem. 2015, 7, 57-64
  7. Tang W#, Dong SH#, Repka LM, He C, Nair SK*, van der Donk WA*. Applications of the class II lanthipeptide protease LicP for sequence-specific, traceless peptide bond cleavage. Chem. Sci. 2015, 6, 6270-6279 (# equal contribution)
  8. Tang W, van der Donk WA*. The sequence of the enterococcal cytolysin imparts unusual lanthionine stereochemistry. Nat. Chem. Biol. 2013, 9, 157-159
  9. Tang W, van der Donk WA*. Structural characterization of four prochlorosins: a novel class of lantipeptides produced by planktonic marine cyanobacteria. Biochemistry 2012, 51, 4271-4279