Transgenic Mouse Facility

Services

  • Production of transgenic mice from injection through the F1 stage
    • Standard strains: CD-1 outbred, C57Bl/6 inbred
    • Almost all non-standard strains can be accommodated, including KO and transgenic models
  • Embryonic Stem Cell Services
    • Blastocyst injections
    • NeoR Timed pregnant females for fibroblast feeder cells
    • TgN(DR4)1Jae timed pregnant females for fibroblast feeder cells
        (drug resistant to G418, 6-thioguanine, puromycin, and hygromycin)
    • Breeding of chimeric mice through the F1 stage
    • ES Cell Lines Available:
      • 129/S6
      • C57B6/T
      • B6-tyrC-2J (B6 white), B6-tyrC-2J /KSR
      • C57BL6/J
      • 129/X1
      • Balb/CJ
      • C3H
  • Embryo aggregations
  • Tail biopsies for genotyping
  • Timed pregnant mice for embryo or newborn use
  • Mouse breeding services and advice
  • Rederivation of non-commercial mice (in conjunction with the ARC)
  • ES cell culture services, including feeder production, ES cell targeting
  • Gene construction for ES cell targeting
  • Isogenic DNA for the preparation of ES cell constructs
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