The core facility, under the direction of Dr. Kay Macleod, provides a large number of services to University of Chicago investigators including: transgenic mouse production including site directed transgenics (TARGATT), transgenic and gene targeting vector design and construction, CRISPR/Cas9 RNA injections, ES cell technology- based mouse production (from blastocyst injection of murine ES cells), and embryo re-derivation in conjunction with the Animal Resources Center (ARC). The facility also provides timed pregnant females of various strains and other mouse breeding services. The facility maintains several lines of mice (FLPe, Mox 2Cre, ROSA26 R/G, Tomato Red/GFP) to provide critical mouse strains at low cost to those investigators generating mice with conditional gene deletions. The state-of-the-art facility maintains all equipment necessary to perform the above operations. The molecular and tissue culture lab is located in the Knapp Center for Biomedical Discovery (KCBD) building and the animal procedure facilities located within the Animal Resources Center barrier facility in the Gordon Center for Integrative Science (GCIS).

The core may be identified in publications as The University of Chicago Transgenic Mouse Facility (RRID:SCR_019171).