Cytometry and Antibody Technology

Benchtop Analyzers

Spectral flow and traditional flow cytometers at the CAT Facility

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Spectral Flow Cytometer

Spectral flow cytometers (SFC) allow the measurement of fluorescence generated by dyes or fluorophores excited by different laser beams. Unlike traditional flow cytometry where each parameter is allocated to a specific detector, the SFC utilize a greater number of detectors then the number of measured parameters and no specific filter is dedicated to any given signal. Instead, the instrument measures the entire emission of each signal generated and unmix the signatures during the sample analysis. This allows for the study of a larger number of parameters simultaneously.

Aurora

Model: Aurora
Manufacturer: Cytek Biosciences

2 identical units available – called Blue and Green (a colored piece of tape is used to differentiate them.)

Spatially separated lasers
– 355nm 20mW
– 405nm 100mW
– 488nm 50mW
– 561nm 50mW
– 640nm 80mW

Plate Loader

Absolute cell count

For more information about the Aurora, please see our resources page

Aurora analysis station
The Cytek SpectroFlo software has been made available on the analysis station located next to the Aurora to facilitate data analysis. The computers have been bridged to facilitate file transfer.

On the Aurora acquisition computer, files located in C:\CytekbioExport\FCSFiles\FlowUser can be found on the Z:\ drive of the Analysis station. Similarly, files copied in the Z:\ drive of the analysis station are saved in the same folder on the acquisition computer.

Note that only the files located in G:\CytekbioExport\FCSFiles\FlowUser are pushed to the CAT Facility server.

Flow Cytometers

Flow cytometers measure the emission of fluorophores and dyes excited by various laser beams. Each signal is directed through an array of mirrors and filters to a specific detector. The number of lasers and the filter configuration can vary between instruments. You will find a full description of each instrument below. More information on recommended fluorophores, brightness indexes, and recommended voltages can be found on the instrument guides page and the panel design page.

Model : LSR Fortessa X20
Manufacturer: BD Biosciences

Spatially separated lasers.
– 488nm 100mW
– 405nm 100mW
– 561nm 100mW
– 640nm 100mW
– 355nm (UV) 15mW

Optical configuration: Fortessa X20 5-18

Located in BSLC room R022

Model : LSR Fortessa
Manufacturer: BD Biosciences

Spatially separated lasers.
– 488nm 50mW
– 405nm 50mW
– 561nm 50mW
– 640nm 40mW

Optical configuration: Fortessa 4-15

Located in BSLC room R022

Model : LSR Fortessa
Manufacturer: BD Biosciences

Spatially separated lasers.

– 488nm 50mW
– 405nm 50mW
– 561nm 50mW
– 640nm 40mW

Optical configuration: Fortessa 4-15 HTS

Plate loader

Located in BSLC room R022

Model: Attune NxT
Manufacturer: ThermoFisher

Spatially separated lasers
– 488nm 100mW
– 405nm 100mW
– 561nm 140mW
– 640nm 140mW

Optical Configuration: AttuneNxT 4-14.PNG

Plater loader

Absolute Cell count

Acoustic Focusing

ThermoFisher Training material

More information

Located in BSLC room R022

Model: Accuri C6
Manufacturer: BD Biosciences

2 spatially separated laser
– 488nm
– 640nm

Optical configuration:
Blue line: 533/30, 585/40, 670LP
Red line: 675/25

Absolute cell count

This model has been discontinued (manufacturer note)

Located in BSLC room R022

Model: BD LSRII
Manufacturer: BD Bioscience

4 spatially separated lasers

– 488nm
– 405nm
– 561nm
– 630nm

Optical Configuration: LSRII 4-12

The manufacturer may no longer support this instrument beyond 2022

Located in Billings Hospital room S301

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