Learn the latest news about the CAT Facility and tips for learning flow cytometry. Feel free to share your opinion in the comments section of each post. The comment section will be moderated for tone and content.
When the global pandemic hit and Chicago’s shelter in place orders prevented me from going into the lab, I started doing some experiments in my kitchen instead. I decided to take on baking sourdough bread (like many other quarantined individuals). And while I...
Laura Johnston | Sep 30, 2020 | 14 Comments
When calculating compensation, automated tools are the gold standard. However, people often struggle to get good results from the automated compensation tools and will turn to manual compensation to fix any errors. Why is it difficult to get accurate results...
Laura Johnston | Jun 24, 2020 | 16 Comments
The voltages on a flow cytometer are one of the more challenging settings to adjust properly. If voltages are too high or too low, the data looks terrible and is potentially unusable. Once voltages have been set and data have been collected there is no way to go...
Laura Johnston | Oct 18, 2019 | 6 Comments
For some reason, it seems like the idea of compensation gets so much 'publicity'. Everyone is always talking about compensation and how difficult it is. New users of flow cytometry tend to think of this idea as something so complex that they end up stumbling on...
Ryan Duggan | Dec 19, 2011 | 0 Comments
I'm not going to discuss the merits of transforming your log-scaled fluorescence flow cytometry data; I'll leave that to the professionals. What I will try to elucidate here is how I tweak the transformation using FlowJo (Mac version 9.3, sorry Windows people)....
Ryan Duggan | Apr 18, 2011 | 0 Comments
For anyone doing sorting applications, I'd like to clear up a common misconception. Just for the record, cell sorters are not sterile. The instrumentation is not housed in a biosafety cabinet or hood, and therefore it is not possible to sort in a STERILE...
Ryan Duggan | Oct 22, 2007 | 0 Comments
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