Learn the latest news about the CAT Facility and tips for learning flow cytometry. Feel free to share your opinion in the comments section of each post. The comment section will be moderated for tone and content.
I've been thinking about brightness in flow cytometry lately. It's a simple enough concept, but it's one of these things where you start thinking about it and you may quickly get lost in the numerous moving parts. One could argue that what people really care about...
David Leclerc | May 27, 2021 | 0 Comments
Two weeks ago I covered my red flags for identifying bad panel design (read that here if you missed it!). This week I’ll go over my red flags for experiment design, which mostly means controls. Controls are needed to be able to appropriately interpret experiment...
Laura Johnston | Dec 3, 2020 | 8 Comments
In the first post of the "bad data" series, I mostly discussed how to identify problems with cytometer settings and compensation by looking at the data plots. (Read that post here in case you missed it!) In this post I’d like to expand on that by going over common...
Laura Johnston | Nov 19, 2020 | 3 Comments
When the global pandemic hit and Chicago’s shelter in place orders prevented me from going into the lab, I started doing some experiments in my kitchen instead. I decided to take on baking sourdough bread (like many other quarantined individuals). And while I...
Laura Johnston | Sep 30, 2020 | 26 Comments
When calculating compensation, automated tools are the gold standard. However, people often struggle to get good results from the automated compensation tools and will turn to manual compensation to fix any errors. Why is it difficult to get accurate results...
Laura Johnston | Jun 24, 2020 | 19 Comments
The voltages on a flow cytometer are one of the more challenging settings to adjust properly. If voltages are too high or too low, the data looks terrible and is potentially unusable. Once voltages have been set and data have been collected there is no way to go...
Laura Johnston | Oct 18, 2019 | 10 Comments
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