Learn the latest news about the CAT Facility and tips for learning flow cytometry. Feel free to share your opinion in the comments section of each post. The comment section will be moderated for tone and content.
The latest ChUG podcast is out where we discuss a number of topics. But I wanted to revisit the unmixing with beads issue that we discussed in a previous post. So it turns out beads create unmixing matrix that do not correct spectral artifacts completely. I'm...
David Leclerc | Apr 1, 2024 | 0 Comments
Should I use cells or beads? This question is at the center of many discussions nowadays in the flow cytometry world. Beads were once lauded as the easy alternative to cells to prepare your compensation controls. But as more and more researchers look at their...
David Leclerc | Dec 7, 2023 | 4 Comments
There's a few items I'd like to share that may help users of the Aurora in the CAT Facility and elsewhere. 1 - The benefits of Cytek Cloud Cytek Cloud is an online platform that offers both a panel and SpectroFlo experiment builder tool. The Panel builder is very...
David Leclerc | Apr 20, 2023 | 0 Comments
I have an unpopular opinion: I love compensation. Usually when I bring up compensation I’m met with a chorus of frustrated noises. But to me compensation is an opportunity to solve a complicated puzzle – and I really love a good puzzle. In this post I’m going to...
Laura Johnston | Aug 9, 2021 | 0 Comments
When calculating compensation, automated tools are the gold standard. However, people often struggle to get good results from the automated compensation tools and will turn to manual compensation to fix any errors. Why is it difficult to get accurate results...
Laura Johnston | Jun 24, 2020 | 19 Comments
Back in 2018, our Cytek Aurora was installed and Cytek came to campus for two days to train us on the instrument. It was all very exciting! A short time passed before researchers really started using the instrument and I was asked to design a panel. From what I...
Laura Johnston | Mar 4, 2020 | 3 Comments
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