Learn the latest news about the CAT Facility and tips for learning flow cytometry. Feel free to share your opinion in the comments section of each post. The comment section will be moderated for tone and content.
I have an unpopular opinion: I love compensation. Usually when I bring up compensation I’m met with a chorus of frustrated noises. But to me compensation is an opportunity to solve a complicated puzzle – and I really love a good puzzle. In this post I’m going to...
Laura Johnston | Aug 9, 2021 | 0 Comments
When calculating compensation, automated tools are the gold standard. However, people often struggle to get good results from the automated compensation tools and will turn to manual compensation to fix any errors. Why is it difficult to get accurate results...
Laura Johnston | Jun 24, 2020 | 14 Comments
Back in 2018, our Cytek Aurora was installed and Cytek came to campus for two days to train us on the instrument. It was all very exciting! A short time passed before researchers really started using the instrument and I was asked to design a panel. From what I...
Laura Johnston | Mar 4, 2020 | 1 Comment
I wouldn't be surprised if the subject of titrating antibodies is one of the least favorite topics among users. We all know someone who skips titrations and just uses the most convenient antibody amount. But as panels get larger, antibody titration becomes much...
Laura Johnston | Nov 15, 2019 | 4 Comments
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