Cytometry and Antibody Technology

Contact Us

Anne Sperling, PhD. – Faculty Director

David Leclerc – Technical Director
dleclerc@bsd.uchicago.edu

Laura Johnston – Associate Scientific Director
ljohnston@bsd.uchicago.edu

Michael Olson – Laboratory Manager
molson@bsd.uchicago.edu

Mandel Davis – Senior Technology Specialist
mdavis4@bsd.uchicago.edu

Robert Ladd – Technology Specialist
rladd@bsd.uchicago.edu

Cytometry and Antibody Technology Facility

Phone: (773) 702-9212

Shipping address:
University of Chicago
BSLC building Room R022
924 E 57th Street
Chicago IL 60637

The CAT Facility has a new help desk email where users can send questions about the core and its instruments. Emails sent to this address will be seen by all CAT Facility staff to facilitate quicker responses and ensure users receive the most relevant advice.

CATHelp@bsd.uchicago.edu

  • Instrument issues outside of business hours
  • Questions about appointments/availability (e.g. sorting)
  • Quick questions about panel design/sample prep
  • Problems with server/data access
  • Confusion about when to use the CATHelp email

CAT Facility description:

Cytometry and Antibody Technologies Facility (CAT):  https://voices.uchicago.edu/ucflow/   This facility is directed by Dr. Anne Sperling. The main facility is located in the Biological Sciences Learning Center and has a satellite facility in the Albert Merritt Billings Hospital.  The CAT Facility serves the faculty by providing access to state-of-the-art technology in quantitative analytical approaches to measure molecular and cellular function. The facility is designed to meet the widespread needs for specialized cytometric analysis and continues to respond to the demand for new and improved technology. The CAT facility supports a Helios mass cytometer, which allows for the detection of 40+ parameters on a cell-by-cell basis. It also supports two 5-laser (355nm, 405nm, 488nm, 561nm and 640nm) Cytek Aurora flow spectral analyzer which allows for the measurement on 40+ fluorophores on every single cell. Currently the main facility employs 5 standard cytometers, including a BD Accuri C6 (488 and 640nm laser with 4 parameter detectors), a ThermoFisher Attune with 4 lasers (405nm, 488nm, 561nm and 640nm) and 14 detectors, two custom 4 laser (405nm, 488nm, 561nm, and 640nm) / 15 parameter detectors BD LSR-Fortessa (one equipped with a 96 well HTS unit) and a LSR-Fortessa X20 equipped with 5 lasers (UV, 405nm, 488nm, 561nm, and 640nm) and 18 parameter detectors. In addition, the CAT facility has 2 BD FACSAriaII high-speed cell sorters with 4 lasers (405nm, 488nm, 561nm and 640nm) and 15 parameter detectors, as well as a BDFACS Aria Fusion cell sorter equipped with 5 lasers (UV, 405nm, 488nm, 561nm, and 640nm) and 18 parameter detectors. This last instrument, incased in a BSL2 laminar flow hood, is dedicated solely for users processing human patient samples in a translational research setting. All of the cell sorters are equipped with 96-well plate robotic arm attachments, various nozzle tip sizes (70um – 130um) and temperature control (4C – 37C). The CAT Facility also hosts a MACSQuant Tyto cell sorter equipped with 3 lasers (488nm, 405nm, and 638nm) and 8 fluorescence detectors which process cells within a closed cartridge, offering full biosafety and protection against microbial contamination. The satellite facility has a custom-built BD LSRII equipped with 4 lasers (405nm, 488nm, 561nm, and 640nm) and 12 parameter detectors. Additionally, the facility has an imaging cytometer, the EMD-Millipore ImageStreamX MkII, which has 4-lasers (405nm, 488nm, 561nm, 640nm) and two 6-channel CCD cameras to collect up to 10-color fluorescence image data from thousands of cells per second. The facility also has a dedicated Luminex bead analyzer from Millipore and a Quickplex SQ120 from Meso Scale Discovery to perform multiplex cytokine assays.  Trained users are provided 24-hour access on any of these instruments with the exception of the Helios mass cytometer, which is operated by the staff of the facility.

Acknowledgment:

[Technique/technology] was performed by the [facility name] at University of Chicago, which receives financial support from the Cancer Center Support Grant (P30CA014599). RRID: SCR_017760

We thank The University of Chicago [facility name], especially [staff name], for their assistance with [technique/technology]. RRID: SCR_017760

Facility RRID: SCR_017760

The ITM wants to help you take advantage of core facility resources, awarding up to $5,000 to offset any costs associated with using one of UChicago’s Core Facilities.

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