Cytometry and Antibody Technology

Instrument Software Training

How to scheduleSoftware Tutorials

Scheduling Hands on Training for Traditional Benchtop Analyzers

All users who wish to use any analyzer (Fortessa, Attune, Quanteon, ImageStream, Aurora) are required to complete the Flow Basics Training course.  If you have not done so already please check the Flow Basics Course page for more information about how to register for the zoom meeting, the upcoming course dates, and a PDF of the presentation. The hands on portion of the training is specific to the software that runs the equipment. Note: there is an additional course required to use the Cytek Aurora spectral analyzer (contact Laura Johnston for more information).

Hands-on training is also completed using zoom so that social distancing is maintained. The hands-on session is now broken into two one hour sessions. In the first session a staff member will guide you through operation of the instrument. On the second session we will supervise you running your own practice samples to make sure you’re comfortable. Once you are trained on a cytometer software you can use any analyzer that runs that software. If you would like to use an analyzer that uses a different software you’ll need to schedule another training session with the staff. The Benchtop analyzer training is free.

If you are a new user, check out the New User Checklist to make sure you’ve completed all of the items for getting started in the CAT Facility.

 

Instrument software

  • BD FACSDiva: Runs the Fortessa 4-15, Fortessa 4-15 HTS, and LSR 4-12
  • Attune Cytometric: Runs the Attune NxT
  • NovoExpress: Runs the Quanteon
  • SpectroFlo: Runs the Aurora (contact Laura Johnston for instructions regarding Aurora training)

Instructions for Scheduling Training

You can book your two 1-hr training sessions on the traditional flow cytometer of your choice via iLab (Fortessa/LSR, Attune, or Quanteon). We have a video tutorial on how to use the iLab scheduler if you need help. Once you’ve booked your time, please let us know the chosen cytometer and scheduled times by emailing CATHelp@bsd.uchicago.edu a minimum of 24 hours in advance to ask if a staff member if available. You are welcome to use the following boilderplate text:

Hello,

I would like to schedule hands on training for the benchtop analyzers. I have booked two sessions on the {insert name of cytometer}. The first session is {insert date and time} and the second session is {insert date and time}. Is a staff member available to train me?

Failure to contact the staff 24 hours prior to your scheduled time may result in cancellation and rescheduling due to staff availability. As a general rule of thumb we always suggest creating any appointments in the iLab scheduler before contacting the staff – appointments can always be adjusted or cancelled if there are conflicts with the staff’s schedule.

Software Tutorials

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