Cytometry and Antibody Technology

Aurora Blue
Aurora Red
Fortessa 4-15
Fortessa 4-15 HTS
Attune NxT 4-14
LSRII 4-12
Penteon (coming soon)
Bigfoot
Aria Fusion 5-18
AriaII 4-15
MACSQuant Tyto
Image Stream MarkII
Helios
MSD Quickplex SQ120
Luminex 200

The nomenclature reflects the instruments as listed in the CAT Facility iLab’s site: Model_# lasers-# detectors

Auxiliary Equipment

Auxiliary equipment is available in the facility complimentarily

Orbital plate shaker
Orbital plate shaker in a 4C cold room
Hand held magnetic bead plate washer
BioTek ELx405 automated magnetic bead plate washer
Vacuum manifold plate washer
Vortemp 56 shaking incubator

GeminiBio Moxi V cell counter (fluidic microchip available at cost)
Millipore Scepter cell counter

Smart Tube Base Station

David and Etta Jonas Center for Cellular Therapy

The CAT Facility manages the Jonas Center flow cytometry equipment located in the S301 satellite facility in the Billings Hospital. Any user can access the instrument, however, Jonas Center members have priority on scheduling every day from 1PM to 5PM. Walk-ins by non-Jonas Center members are allowed when the instrument is not booked during that period.

Model: NovoCyte Quanteon
Manufacturer: Agilent

 

Spatially separated lasers

– 405nm 100mW
– 488nm 100mW
– 561nm 100mW
– 637nm 100mW

NovoSampler Q compatible with 40 tube rack, and 24- 48- 96, and 384-well plate

Absolute cell count

Pritzker School of Molecular Engineering Instruments

The CAT Facility manages the flow cytometry equipment located in the PME research space. Non-PME users should book instruments located in the main lab primarily. Access to the William Eckhardt Research center requires proper ID card authorization.

Model: Aurora
Manufacturer: Cytek Biosciences

Spatially separated lasers

– 355nm 20mW
– 405nm 100mW
– 488nm 50mW
– 561nm 50mW
– 640nm 80mW

Plate Loader

Absolute cell count

For more information about the Aurora, please see our resources page

Model : LSR Fortessa
Manufacturer: BD Biosciences

Spatially separated lasers.

– 488nm 100mW
– 405nm 100mW
– 561nm 100mW
– 640nm 100mW
– 355nm (UV) 15mW

Plate loader

Optical configuration: PME Fortessa

The BSLC R022 main lab