Aurora Panel Design TutorialSpectral Flow Cytometry
Panel Design Overview
- Choose markers
- Choose fluorophores
- Pair markers and fluorophores using Aurora-specific tools
- Test panel on cells of interest
The video below provides a tutorial for designing an Aurora panel. There are also several papers that provide excellent guides for panel design and optimization. For tools to assist in panel design, see the Panel Design Tools resource.
The first paper provides a guide for designing a panel, while the second paper goes through the process of optimizing the panel and checking the quality to determine if all populations and markers of interest can be properly resolved. For a full workflow on setting up an Aurora experiment from start to finish, check out the Experiment Design resource.
Panel Design and Optimization for High-Dimensional Immunophenotyping Assays Using Spectral Flow Cytometry.
Ferrer-Font L, Pellefigues C, Mayer JU, Small SJ, Jaimes MC, Price KM. Curr Protoc Cytom. 2020 Mar;92(1):e70. doi: 10.1002/cpcy.70. PMID: 32150355.
Panel Optimization for High-Dimensional Immunophenotyping Assays Using Full-Spectrum Flow Cytometry
Ferrer-Font L, Small SJ, Lewer B, Pilkington KR, Johnston LK, Park LM, Lannigan J, Jaimes MC, Price KM. Curr Protoc. 2021 Sep;1(9):e222. doi: 10.1002/cpz1.222. PMID: 34492732.