Aurora Panel Design ToolsSpectral Flow Cytometry
This page contains tools to assist in panel design for the Aurora spectral cytometer. For a tutorial on panel design theory and tips for optimizing an Aurora panel, see the Panel Design Tutorial resource.
Full Cytek Fluorochrome Selection Guideline
Cytek’s guide to fluorophores on the Aurora – see expected signatures, brightness indexes, spread matrix, etc. Individual components from this pdf are saved below.
30 Color Spread Matrix with Percentages
How to read this table: the fluor in the row impacts the one in the column. Areas in bright pink and red is where more attention to panel design is needed. This table was created with CD4 reference controls for all fluorophores. This table assumes all markers are bright and all markers are in the tube.
Perpetrators and Victims
An alternative way of looking at the spread matrix. This tool is based on the 30-color spread matrix – it was created by counting either the rows or columns and determining how many boxes contained values above 0%, 30%, or 50%.
The stain index is used to estimate fluorohore brightness. When designing a panel, make sure that low expressed markers are paired with bright fluorophores for optimal results.
A quick guide to fluorophores that can be used together in a panel. Fluorophores listed in the same row should not be used in a panel together – the unmixing algorithm will not be able to distinguish them.
List of Tandem and Polymer Dyes
A quick guide to tandem and polymer fluorophores. You can also check manufacturer websites to determine if a fluorophore is a tandem dye or a polymer dye.
Links to External Resources
Easy Panel Experiment Design Platform
Our recommended panel design tool. Automatically pairs your markers with the most appropriate fluorophores by optimizing the complexity and global similarity index.
Fluorofinder Experiment Design Platform
Online panel design tool. Also contains a spectra viewer, as well as an antibody and fluorophore database.
Cyteks’ panel builder. You can also use the top to create and export an experiment file that can be used on the Aurora spectral analyzers.