Flow Basics 2.0Supplementary Training Course
Flow Basics 2.0 is a series of courses that builds on the original Flow Basics course. This series outlines all of the practical steps for setting up a flow cytometry experiment and highlights factors that impact final results. The videos can be watched on our YouTube channel and the slides can be downloaded below.
Flow Basics 2.1: Staining Protocol and Reproducibility
- Overview of a basic cell surface marker staining protocol
- How does Fc block work
- Five factors that affect antibody staining
- What is the difference between the available viability dyes
- Download Flow Basics 2.1
Flow Basics 2.2: Optimizing the Staining Protocol
- Why you should optimize tissue digestion
- Alternative blocking options
- How to determine the number of cells to stain for an experiment
- Why and how to perform antibody titration
- Download Flow Basics 2.2
Flow Basics 2.3: Panel Design
- Information needed before beginning panel design
- Tools available for for panel design
- How to assign markers to fluorophores
- Which fluorophores to avoid
- Strategies for placing high spillover fluorophores
- Download Flow basics 2.3
Flow Basics 2.5: Instrument setup and Automated Compensation
- Why organizing the experimental layout is useful
- Shortcuts for labeling tubes quickly in BD FACSDiva
- How to set voltages on the cytometer
- How to properly use automated compensation tools and avoid compensation errors
- How to identify compensation errors
- Download Flow Basics 2.5
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