Sample Preparation Guides
Work in progressGeneral Tips for Sample Preparation
It’s pretty easy to find a lot of great protocols for sample preparation. Our goal is to help you understand these sample preparation protocols in order to get good quality data. Download our slides to understand:
- Factors within the staining protocol affect data quality:
- Staining time, temperature, volume, cell number, antibody amount
- Washing
- Buffers and fixatives
- Pipetting error
- Blocking and dead cell discrimination
- Other considerations include:
- Digestion of tissue into single cells
- Gating controls for data analysis
- Staining controls for compensation and instrument setup
- Determining the appropriate number of cells required for the experiment
- Maintaining consistency in order to achieve reproducible results





