Cytometry and Antibody Technology

Sample Preparation Guides

Work in progress

General Tips for Sample Preparation

It’s pretty easy to find a lot of great protocols for sample preparation. Our goal is to help you understand these sample preparation protocols in order to get good quality data. Download our slides to understand:

  • Factors within the staining protocol affect data quality:
    • Staining time, temperature, volume, cell number, antibody amount
    • Washing
    • Buffers and fixatives
    • Pipetting error
    • Blocking and dead cell discrimination
  • Other considerations include:
    • Digestion of tissue into single cells
    • Gating controls for data analysis
    • Staining controls for compensation and instrument setup
    • Determining the appropriate number of cells required for the experiment
    • Maintaining consistency in order to achieve reproducible results