Wei-Jen Tang Laboratory

Collaborations at UChicago

Aaron Dinner

Aaron Dinner’s lab offers the mathematic framework to understand the behavior of chemokine polymerization.

Key publications

Polymerization of MIP-1 chemokine (CCL-3 and CCL-4) and clearance of MIP-1 by insulin degrading enzyme

Structural basis for oligomerization and glycosaminoglycan-binding of CCL5 and CCL3. 

Dinner lab website: https://dinner-group.uchicago.edu/

David Kovar

David Kovar is our resident actin and TIRF microscopy expert. Our work revealed that ExoY, a toxin produced by Pseudomonas aeruginosa, directly bundles actin filaments.

Key publications

Pseudomonas aeruginosa exoenzyme Y directly bundles actin filaments.

Kovar lab website: https://voices.uchicago.edu/kovarlab/

Deborah Nelson

We have collaborated to understand the regulation of ion channels by G proteins.

Key publications

The Inwardly Rectifying K+ Channel Subunit GIRK1 Rescues the GIRK2 weaver Phenotype

Nelson lab website: https://voices.uchicago.edu/nelsonlab/

Tobin Sosnick

We explore unfoldase activity of insulin degrading enzyme using ubiquitin and mutants

Key publications

Ubiquitin Is a Novel Substrate for Human Insulin-Degrading Enzyme

Sosnick lab website: http://sosnick.uchicago.edu/

Minglei Zhao

Minglei Zhao is our cryoEM expert. He taught us everything we know about single particle analysis and solves all the weird problems we can’t figure out.

Key publications

Structural analysis of Mycobacterium tuberculosis M13 metalloprotease Zmp1 open states

Zhao lab website: https://zhaolab.uchicago.edu/

Collaborations outside UChicago

Bridget Carragher and Clinton Potter

Bridget and Clint have taught us several state-of-the-art cryoEM/ET techniques that we have utilized to gain structural insights into macromolecular complexes.

Key publications

Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme

Affiliation(s):

National Resource for Automated Macromolecular Microscopy (NRAMM): https://nramm.nysbc.org/

National Center for CryoEM Access and Training (NCCAT): https://nccat.nysbc.org/

National Center for In-situ Tomographic Ultramicroscopy (NCITU): https://ncitu.nysbc.org/

Columbia University: Bridget, Clint

Jan Florian


We collaborate to address the catalytic mechanism of HIV protease using MD simulation

Key publications

Insights from Atomic-Resolution X-Ray Structures of Chemically Synthesized HIV-1 Protease in Complex with Inhibitors

Affiliation: Loyola University

 

Pierre Goossens and Michele Mock


We collaborate to study how anthrax toxins work.

Key publications

Noninvasive Imaging Technologies Reveal Edema Toxin as a Key Virulence Factor in Anthrax

Affiliation: Pasteur Institute

 

Andrzej Joachimiak


We collaborate for the structural analysis of human insulin degrading enzyme

Key publications

Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism

Affiliations: Advanced Photon Source, Argonne National Laboratory

                        APS Structural Biology Center

 

Sheng Li

Sheng Li is an expert in hydrogen deuterium exchange mass spectrometry who works with us to understand the interaction between enzymes and substrates

Key publications

Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme

Affiliations: University of California, San Diego

 

David Liu

We collaborate to discover inhibitors of of human insulin degrading enzyme.

Key publications

Anti-diabetic activity of insulin-degrading enzyme inhibitors mediated by multiple hormones

Affiliations: Harvard University

 

Dennis Selkoe

We collaborate to discover inhibitors of human insulin degrading enzyme.

Key publications

Designed inhibitors of insulin-degrading enzyme regulate the catabolism and activity of insulin

Affiliations: Harvard University

Roland Siefert

We collaborate to study the nucleotidyl cyclase activity of bacterial enzymes.

Key publications

Cytidylyl and Uridylyl Cyclase Activity of Bacillus anthracis Edema Factor and Bordetella pertussis CyaA

Affiliations: Medizinische Hochschule Hannover