Aurora, Spectral Flow Cytometry Analyzer

Aurora is a new type of flow cytometry analyzer; it works by measuring full emission spectrum from each laser (thus the name: spectral flow cytometer) and uses unmixing algorithm to calculate individual fluorochrome contribution within emission of all fluorochromes in flow cytometry panel of antibodies.

Practically it means less restriction in fluorochromes options, ability to combine fluorochromes with similar emission profile which at the end allow us to create bigger panels with great signal to noise ration.

With 64 individual detectors theoretical number of markers analyzed simultaneously on Aurora is 62, currently we are using 42 colors high quality and modular flow cytometry panel for blood samples on Aurora. Similar panels are in development for different tissues.

Configuration:

5 lasers, 64 detectors (channels)

UV laser at 355nm, 20mW, 16 channels covering spectrum from 365 to 829 nm.,

Violet laser at 405nm, 100mW, 16 channels from 420 to 829 nm.

Blue laser at 488nm, 50mW, 14 channels from 498 to 829 nm.

Yellow-Green laser at 561nm, 50mW, 14 channels from 567 to 829 nm.

Red laser at 640nm, 80mW, 8 channels from 652 to 829 nm.

Aurora is also equipped with plate loader which allows for automated runs of up to 96 samples at once.

New and very useful function on Aurora is volumetric sensor which allows for absolute quantification of any gated populations (counts per ul)

Good starting point for new Aurora users is Cytek website:

https://cytekbio.com/pages/aurora#tab-overview

Great tool for panel design for Aurora is full spectrum viewer:

https://spectrum.cytekbio.com/

And of course any additional questions related to Aurora will be answered by HDID core staff, we can also schedule more advanced training and address questions related to specific project.