Human Disease and Immune Discovery

Contact Us

Bana Jabri, MD, PhD – Faculty Director
bjabri@uchicago.edu
773-834-8632

Cezary Ciszewski – Technical Director
cciszews@bsd.uchicago.edu
773-987-8798

Human Disease & Immune Discovery Core Facility

Location: KCBD 1st floor, room 1260 (North end of the building)

900 E 57th St
Chicago, IL 60637

Email: hdid@bsd.uchicago.edu; cciszews@bsd.uchicago.edu

Slack : hdid-workspace.slack.com; cciszews

Microsoft Teams: HDID Core Facility, cciszew@uchicago.edu

Phone: 773 834 6927

This facility is directed by Professor Bana Jabri and is located on the 1st floor of Knapp Center for Biomedical Discovery.  The HDID Facility serves the scientific community by providing access to 2 state of the art multiparameter flow cytometry instruments.

Aurora from Cytek is a spectral flow cytometry analyzer. Equipped with 5 lasers: UV 355nm, Violet 405nm, Blue 488nm, Yellow-Green 561nm, Red laser at 640nm and 64 fluorescence detectors covering the whole spectrum of emission from each laser line. This instrument was successfully used to generate data based on the staining with panel of 42, and theoretical maximum number of fluorochroms that Aurora instrument can quantify simultaneously is 62.

S6 FACSymphony cell sorter is the newest generation instrument from BD Biosciences. It’s equipped with 5 lasers and 30 fluorescence detectors (100mW UV laser at 355nm – 8 detectors; 100mW Violet laser at 408nm – 8 detectors; 150mW Blue laser at 488nm – 6 detectors; 150mW Yellow-Green laser at 561nm – 5 detectors; 140mW Red laser at 638nm – 3 detectors). In addition to highly multiparameter capabilities S6 cell sorter can be used to physically separate up to 6 unique cell population simultaneously (6-way sorting).

HDID core facility is also well equipped with modern laboratory equipment that allow for samples processing and antibodies staining prior flow cytometry acquisition. We also provide access to powerful computer workstation that is used for data analysis and visualization.