Human Immunologic Monitoring Facility

Fees

 

Sample Processing & Storage  Unit Internal Rate External Rate
-80C storage per vial per month  $                        2  $                    4
CD8+ or CD3+ separation each sample  $                    129  $                317
cfDNA isolation sample  $                      56  $                138
Germline DNA Extraction from PAXgene tube  $                      96  $                236
Liquid nitrogen storage per vial per month  $                        5  $                  11
Monthly Sample Cryogenic Storage Fee month  $                    120  $                297
Patient Biopsy Processing each  $                      34  $                  83
PBMCs from 1 X CPT each  $                      93  $                229
PBMCs from 4 X CPT each  $                    135  $                332
PMBCs (100 ml blood; Lymphoprep) each  $                    223  $                550
PMBCs (20 ml blood; Lymphoprep) each  $                    115  $                283
PMBCs (50 ml blood; Lymphoprep) each  $                    146  $                359
Serum/plasma each  $                      27  $                  65
Stool banking sample (including kit+ 3x aliquots)  $                      41  $                100
       
Single-cell RNA library preparation      
10X  Hashing Tag Add-On per library  $                    170  $                420
10x Chronium iX Usage (self service) per run  $                    146  $                361
10X Feature Barcoding Add-On per library  $                    170  $                420
10x Genomics Single Cell RNAseq 3′ library preparation add-on cost per sample (10x material not included) per sample  $                    146  $                360
10x Genomics Single Cell RNAseq 3′ library preparation base (10x material not included) batch (up to 8 samples, 1x library each)  $                    732  $             1,808
       
Spatial transcriptome      
10x CytAssist Training per session  $                    188  $                465
10x CytAssist Usage (self service) unit  $                    176  $                433
10x CytAssist Visium/VisiumHD library preparation with H&E imaging add-on cost per sample (10x material not included) per tissue slide  $                    250  $                618
10x CytAssist Visium/VisiumHD library preparation with H&E imaging base (10x material not included) batch (up to 2 tissue slides, 1x library each)  $                 1,476  $             3,646
Evos M7000 (drop off) per slide  $                      60  $                148
Evos M7000 usage (self service) per 30-min  $                      69  $                171
       
Multiples Immunofluorescence (mIF)      
6-plex mIF Staining (reagent cost not included) batch (up to 12 slides)  $                    720  $             1,780
Optimization mIF Staining (reagent cost not included) per target per slide  $                    110  $                272
Single Target IF Staining (Ab cost not included) batch (up to 12 slides)  $                    200  $                495
Triple Targets IF Staining (Ab cost not included) batch (up to 12 slides)  $                    480  $             1,187
Vectra Polaris Scanner (Self-service) 30 mins  $                      43  $                107
Image data analysis per 30-min  $                      60  $                149
Image data station (HIM-010) per 30-min  $                      37  $                  92
mIF library per dye per slide  $                      58  $                144
Validation1 mIF Staining & analysis (reagent cost not included) – drop test (FMO) per target  $                    200  $                495
Validation2 mIF Staining & analysis (reagent cost not included) – compare with IHC per target  $                    250  $                618
Vectra Polaris whole slide scanning per slide  $                      39  $                  97
       
Multiples Immunofluorescence (CODEX)      
Antibody conjugation service add-on cost per antibody per antibody  $                      36  $                  88
Antibody conjugation service base up to 6x (Antiboies and Akoya Reagents not included) per batch  $                    251  $                619
FFPE CODEX staining (up to 40+ markers, Akoya reagents not included) per coverslip  $                    893  $             2,205
       
RNAscope (mIF)      
Multiplex RNAscope base (ACD and Akoya reagent cost not included) batch (up to 4 targets; 10 slides)  $                    860  $             2,126
Add-on targets per RNA target per batch (up to 10 slides)  $                      60  $                149
Add-on IF (Akoya reagent cost not included) per batch (up to 10 slides)  $                    280  $                692
Optimization IF Staining (reagent cost not included) per target per slide  $                    308  $                760
       
Molecular Analysis      
DNA/RNA Extraction from FFPE sample  $                    183  $                453
PCR/experiment run  $                      94  $                232
Reverse transcript (cDNA synthesis) each  $                      52  $                128
RNA isolation (PBMCs) each  $                      74  $                182
RNA isolation (Tissue Biopsy) each  $                    104  $                256
Viral RNA Isolation sample  $                      32  $                  79
QPCR add-on cost per reation per rxc  $                        6  $                  13
QPCR base cost run  $                    146  $                360
RNA only Extraction from FFPE sample  $                    126  $                311
DNA Extraction (Fecal Sample) sample  $                    136  $                337
       
Standard Assays      
ELISA each  $                    217  $                535
ELISPOT (basic) plate  $                    326  $                804
ELISPOT reader hour  $                      24  $                  58
multiplex ELISA assay plate  $                    279  $                689
nCounter Usage Full Service run  $                    223  $                551
nCounter Usage Self-Service run  $                    168  $                413
Project Set Up Fee project  $                    319  $                787
Western Blot each  $                    286  $                706
       
Training      
Evos M7000 Training per session  $                    148  $                366
Vectra Polaris Initial Training unit  $                    180  $                445
Image data analysis software training per 15-min  $                      39  $                  97
       
Others      
Consultation per 15-min  $                      30  $                  75
Misc each  $                        1  $                    1
Labor fee 1 per 30-min  $                      25  $                  62
Labor fee 2 per 30-min  $                      38  $                  93
       
FACS Analysis      
HLA-A2 Screening each  $                    112  $                275
Intracellular staining (L5 and IFN-g) each  $                    188  $                463
Intracellular staining (T-reg) each  $                      87  $                214
Surface staining (CD3,14,15,19,56) each  $                    133  $                327
Surface staining (CD3,4) each  $                      98  $                240
T2 Binding Assay each  $                    142  $                350
Tetramer staining each  $                    168  $                414

 

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