Tony Kossiakoff is the king of synthetic antibodies that have facilitated several structural studies.

Key publications

Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme

Locking the Elbow: Improved Antibody Fab Fragments as Chaperones for Structure Determination

Rapid Discovery and Characterization of Synthetic Neutralizing Antibodies against Anthrax Edema Toxin

Kossiakoff lab website: https://kosslab.uchicago.edu/

David Kovar is our resident actin and TIRF microscopy expert. Our work revealed that ExoY, a toxin produced by Pseudomonas aeruginosa, directly bundles actin filaments.

Key publications

Pseudomonas aeruginosa exoenzyme Y directly bundles actin filaments.

Kovar lab website: https://voices.uchicago.edu/kovarlab/

We have collaborated to understand the regulation of ion channels by G proteins.

Key publications

The Inwardly Rectifying K⁺ Channel Subunit GIRK1 Rescues the GIRK2 weaver Phenotype

Nelson lab website: https://voices.uchicago.edu/nelsonlab/

We collaborate to understand how anthrax edema factor and human insulin degrading enzyme work.

Key publications

Anthrax edema factor potency depends on mode of cell entry

The C-terminal domain of human insulin degrading enzyme is required for dimerization and substrate recognition

Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism

Anthrax Edema Toxin Inhibits Endothelial Cell Chemotaxis via Epac and Rap1

Rosner lab website: https://rosnerlab.com/

We explore unfoldase activity of insulin degrading enzyme using ubiquitin and mutants

Key publications

Ubiquitin Is a Novel Substrate for Human Insulin-Degrading Enzyme

Sosnick lab website: http://sosnick.uchicago.edu/

We help each other to better our research.

Key publications

In vivo epidermal migration requires focal adhesion targeting of ACF7

Rapid Discovery and Characterization of Synthetic Neutralizing Antibodies against Anthrax Edema Toxin

Wu website: https://benmay.uchicago.edu/faculty/xiaoyang-wu-phd

Minglei Zhao is our cryoEM expert. He taught us everything we know about single particle analysis and solves all the weird problems we can’t figure out.

Key publications

Structural analysis of Mycobacterium tuberculosis M13 metalloprotease Zmp1 open states

Zhao lab website: https://zhaolab.uchicago.edu/

We collaborate to characterize small molecule modulators of human insulin degrading enzyme.

Key publications

Catalytic site inhibition of insulin-degrading enzyme by a small molecule induces glucose intolerance in mice

Identification of ebselen as a potent inhibitor of insulin degrading enzyme by a drug repurposing screening

Imidazole-derived 2-[N-carbamoylmethyl-alkylamino]acetic acids, substrate-dependent modulators of insulin-degrading enzyme in amyloid-β hydrolysis

Structure–activity relationships of imidazole-derived 2-[N-carbamoylmethyl-alkylamino]acetic acids, dual binders of human insulin-degrading enzyme

Affiliation: Pasteur Institute

We collaborate to address the catalytic mechanism of HIV protease using MD simulation

Key publications

Insights from Atomic-Resolution X-Ray Structures of Chemically Synthesized HIV-1 Protease in Complex with Inhibitors

Affiliation: Loyola University

We collaborate to study how anthrax toxins work.

Key publications

Noninvasive Imaging Technologies Reveal Edema Toxin as a Key Virulence Factor in Anthrax

Affiliation: Pasteur Institute

We collaborate for the structural analysis of human insulin degrading enzyme

Key publications

Structures of human insulin-degrading enzyme reveal a new substrate recognition mechanism

Affiliations: Advanced Photon Source, Argonne National Laboratory

                        APS Structural Biology Center

Sheng Li is an expert in hydrogen deuterium exchange mass spectrometry who works with us to understand the interaction between enzymes and substrates

Key publications

Ensemble cryoEM elucidates the mechanism of insulin capture and degradation by human insulin degrading enzyme

Affiliations: University of California, San Diego

We collaborate to discover inhibitors of of human insulin degrading enzyme.

Key publications

Anti-diabetic activity of insulin-degrading enzyme inhibitors mediated by multiple hormones

Affiliations: Harvard University

We collaborate to discover inhibitors of human insulin degrading enzyme.

Key publications

Designed inhibitors of insulin-degrading enzyme regulate the catabolism and activity of insulin

Affiliations: Harvard University

We collaborate to study the nucleotidyl cyclase activity of bacterial enzymes.

Key publications

Cytidylyl and Uridylyl Cyclase Activity of Bacillus anthracis Edema Factor and Bordetella pertussis CyaA

Affiliations: Medizinische Hochschule Hannover