The University of Chicago

Advanced Electron Microscopy

Titan Krios

Equipped with a Gatan K3 detector and BioQuantum GIF, the Krios acquires high-resolution images at cryogenic temperatures.

Prestin’s conformational cycle underlies outer hair cell electromotility

Map and the overall structure of the dolphin SLC26A5 homodimer at
3.3 Å nominal resolution when Cl- is the main anion.
Bavi, N. et al. Prestin’s
conformational cycle underlies outer hair
cell electromotility. Nature https://doi.
org/10.1038/s41586-021-04152-4 (2021).

Structural analysis of M. tuberculosis M13 metalloprotease Zmp1 open states

Map of open state at 3.1Å resolution.
Liang et al., Structural analysis of Mycobacterium tuberculosis
M13 metalloprotease Zmp1 open states, Structure(2020),
https://doi.org/10.1016/j.str.2020.12.002

Volumescope 2

Volumescope 2 SEM –

Serial block face imaging

for large volume samples.

Aquilos Cryo-FIB

With a cryo-DualBeam system,

the Aquilos prepares frozen thin samples for

high-resolution tomographic imaging

Acknowledge Us!

We track publications in which the Advanced Electron Microscopy Core helped in the collection of data or images. Help us by acknowledging us in your work and sending us your publication to be featured on our site.
Acknowledging the facility and listing the core equipment used helps demonstrate how our core contributes to the university community and track the impact of each piece of equipment on research infrastructure.

The Core can be cited as follows:
The Advanced Electron Microscopy Core may be identified in publications as The University of Chicago Advanced Electron Microscopy Core Facility (RRID:SCR_019198).

Our BoilerplateText is also available.

Welcome to the Advanced Electron Microscopy Facility

The Advanced Electron Microscopy Facility occupies over 2000 ft2 in the Gordon Center for Integrative Sciences (GCIS) and an additional 3000 ft2 of new space in the Franklin McLean Institute. The mission of the facility is to develop techniques for preserving cellular structure with the highest degree of reliability. These techniques involve different methods for rapidly freezing our samples in order to halt structural and biochemical activity in a very short time-frame, thus preserving structure in the “live” state. Once the sample is preserved in the “live” state, it is then possible to study the ultrastructure of these samples using not only basic Electron Microscopy imaging techniques, but also state-of the-art techniques such as: 1) 3-D electron tomography (cryo and plastic), 2) Single Particle Analysis (SPA), and 3) CLEM.

Access Your Data

Each person is required to obtain their own login credentials for our data server. To gain access, email our IS Manager Joe Mecyssine (jmecyssi@osrfmailsrv.uchicago.edu). Be sure to include:

1. Your Name (and CNET ID if you have one).

2. That you need access to the Electron Microscopy server. It will be helpful to include whether this is cryo (Krios/Glacios), volume (Apreo), cryo-FIB (Aquilos), and/or general TEM (Spirit/F30).

3. Name of folder you need access to (PI’sLastName_FirstInitial). 

For Publications

We track publications in which the Advanced Electron Microscopy Core helped in the collection of data or images. Help us by acknowledging us in your work and sending us your publication to be featured on our site.
Acknowledging the facility and listing the core equipment used helps demonstrate how our core contributes to the university community and track the impact of each piece of equipment on research infrastructure.

The Core can be cited as follows:
The Advanced Electron Microscopy Core may be identified in publications as The University of Chicago Advanced Electron Microscopy Core Facility (RRID:SCR_019198).

Our Boilerplate Text is also available.

Equipment Status

The Advanced EM works hard to ensure all our equipment is up and running for user’s needs.
In the event that the equipment you need is unavailable or has ongoing issues, we will update the equipment status page to keep you updated.

Check Equipment Status

Krios and Glacios Loading Submission Forms

If you have an upcoming session Krios or Glacios, please remember
to fill out the Screening Submission Form or the Data Collection Submission Form and send it to us on Slack.
External users: Contact core staff for guidelines.

Any questions? Connect with us by email:
tlavoie@uchicago.edu
jotham@uchicago.edu