Equipped with a Gatan K3 detector and BioQuantum GIF, the Krios aquires high-resolution images at cryogenic temperatures.
Structural analysis of M. tuberculosis M13 metalloprotease Zmp1 open states
Map of open state at 3.1Å resolution.
Liang et al., Structural analysis of Mycobacterium tuberculosis
M13 metalloprotease Zmp1 open states, Structure(2020),
With a cryo-DualBeam system,
the Aquilos prepares frozen thin samples for
high-resolution tomographic imaging
Volumescope 2 SEM –
Serial block face imaging
for large volume samples.
Welcome to the Advanced Electron Microscopy Facility
The Advanced Electron Microscopy Facility occupies over 2000 ft2 in the Gordon Center for Integrative Sciences (GCIS) and an additional 3000 ft2 of new space in the Franklin McLean Institute. The mission of the facility is to develop techniques for preserving cellular structure with the highest degree of reliability. These techniques involve different methods for rapidly freezing our samples in order to halt structural and biochemical activity in a very short time-frame, thus preserving structure in the “live” state. Once the sample is preserved in the “live” state, it is then possible to study the ultrastructure of these samples using not only basic Electron Microscopy imaging techniques, but also state-of the-art techniques such as: 1) 3-D electron tomography (cryo and plastic), 2) Single Particle Analysis (SPA), and 3) CLEM.