Ｉ Acknowledging the Facility or the Technical Directors and listing the Core equipment you used in the Materials and Methods section helps us demonstrate how much our Core contributes to the University community and track the impact of each piece of equipment on research infrastructure.

Below are sample sentences to use in the Acknowledgements and Materials and Methods sections.

Detailed protocol descriptions can be listed and will be provided by the Technical Directors.

General Facility Acknowledgement

Imaging (and/or image processing and/or data analysis) was performed at the University of Chicago Integrated Small Animal Imaging Research Resource. You may also acknowledge special contributions to imaging or image analysis by our staff.

Cancer Center members should acknowledge funding received by the University of Chicago Comprehensive Cancer Center through Support Grant P30CA014599.

When critical and/or significant contribution to the project has been provided by Core personnel, authorship should be considered.

Optical Imaging Core

Bioluminescence and/or Fluorescence imaging was performed at the University of Chicago Integrated Small Animal Imaging Research Resource on an IVIS Spectrum (PerkinElmer, Waltham, MA). Animals were anesthetized at 2% isoflurane throughout the acquisition. Imaging analysis was performed using Living Image 4.7.3.

Ultrasound Core

Images were acquired on the S-Sharp (New Taipei City, Taiwan) Prospect 3.0 with a 20Hz (for rats) or 40Hz (for mice) probe. [Acquisition parameters should be listed here.] Animals were anesthetized at 2% isoflurane throughout the acquisition and shaved with depilatory cream. Physiological monitoring was provided by the onboard software.

MRIS Core

MRI experiments were performed on a 9.4 Tesla Bruker (Billerica, MA, USA) small animal scanner with 11.6 cm inner diameter and actively shielded gradient coils (maximum constant gradient strength for all axes: 230 mT/m). After being fitted with physiological monitoring equipment, mice were set-up on the animal bed and placed in the magnet.

[Acquisition protocols will be provided, as well as image analysis protocol when applicable.] 

microPET/SPECT/CT Core

Users of the core should acknowledge funding from NIH S10OD025265-01.

microCT

Mice were imaged by the Integrated Small Animal Imaging Research Resource (iSAIRR) at the University of Chicago on the X-Cube (Molecubes, Ghent, Belgium).  Animals were anesthetized via inhalation with 2% isoflurane. Respiration rate and body temperature of the animal were constantly monitored during the entire scanning period with X-Cube onboard monitoring system.

[Acquisition protocols will be provided, as well as image analysis protocol when applicable.]

microPET/CT

Mice were imaged by the Integrated Small Animal Imaging Research Resource (iSAIRR) at the University of Chicago on a β-Cube and X-Cube (Molecubes, Ghent, Belgium). Animals were anesthetized via inhalation with 2% isoflurane. Respiration rate and body temperature of the animal were constantly monitored during the entire scanning period with the β-Cube and X-Cube onboard monitoring system.

All image analysis was performed with VivoQuant software (inviCRO LLC, Boston, MA, USA). 3D regions of interest (ROIs) were drawn and standardized uptake values (SUVs) and percent injected dose per gram of tissue (%ID/g) were calculated for each ROI and time point. %ID/g was calculated as a ratio of tissue radioactivity concentration (mCi/g) at time of scan to total injected activity (mCi) at time of scan.

[Acquisition protocols will be provided, as well as image analysis.]

microSPECT/CT

Mice were imaged by the Integrated Small Animal Imaging Research Resource (iSAIRR) at the University of Chicago on a 𝞬-Cube and X-Cube (Molecubes, Ghent, Belgium).  Animals were anesthetized via inhalation with 2% isoflurane. Respiration rate and body temperature of the animal were constantly monitored during the entire scanning period with the 𝞬-Cube and X-Cube onboard monitoring system.

All image analysis was performed with VivoQuant software (inviCRO LLC, Boston, MA, USA). 3D regions of interest (ROIs) were drawn and standardized uptake values (SUVs) and percent injected dose per gram of tissue (%ID/g) were calculated for each ROI and time point. %ID/g was calculated as a ratio of tissue radioactivity concentration (mCi/g) at time of scan to total injected activity (mCi) at time of scan.

[Acquisition protocols will be provided, as well as image analysis protocol.]

Allegro PET Scanner – Large Animals

Animals were imaged by the Integrated Small Animal Imaging Research Resource (iSAIRR) at the University of Chicago on a Philips Allegro PET scanner.

[Acquisition protocols will be provided, as well as image analysis protocol.]