Nano String
Nano string is a platform which allows for gene expression profiling of small, discrete regions of a tissue sample. Tissue samples are placed on special microscope slides, and washed with specific RNA-hybridizing probes, which bind in-situ. Slides are then immuno-stained for visualization purposes. The client is able to select regions of interest (ROI), which are extracted from the slide and collected. Sequencing of the collected samples allows for gene expression profiling of small, discrete regions. We are able to accommodate all NanoString assays kits (Cancer transcriptome atlas, whole transcriptome atlas, protein, custom kits). For more information, please visit the vendor’s website: NanoString: Experience the Power of Spatial Biology
Nano string experiments are arranged in advance and tailored to our clients’ needs. The following is our general procedure for facilitating NanoString experiments with clients:
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The client selects a NanoString kit which meets their research goals.
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Client submits a NanoString request form to the facility.
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Due to the high cost of reagents and the fact that NanoString reagents have a limited shelf life, we do not stockpile kits at out facility. As such we require the client to purchase the kit themselves.
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Client prepares tissue samples.
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Client brings the kit and tissue samples to our facility (1230C in the Knapp Center for Biological Discovery).
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Our technician prepares the slides for ROI selection.
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Client is brought to the facility again for visualization and selection of an ROI.
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ROI sample is processed into an NGS library and sequenced at our facility.
Please contact us at nanostring@bsd.uchicago.edu for questions or project initiation.
Pricing:
Please see our pricing page for more details on pricing.
To get a quote, please email (bsdgenomicsconsult@uchicagomedicine.org)