Integrated Light Microscopy Core

To access a microscope, click the New User button above to go to our New User Training Checklist.Trained users have 24/7 access to the facility and are given permission to schedule their own microscope sessions through our online scheduling software

MICROSCOPES BY TYPE AND FUNCTION

The following microscopes are capable of superresolution imaging. They cover several different microscope and superresolution types and are also listed by type in other tabs

Leica GSD/TIRFM inverted ground state depletion superresolution with TIRF

Leica SP8 inverted laser scanning confocal with white light laser, 3D STED and FLIM

3i SoRa Marianas inverted spinning disk confocal

3i Lattice Lightsheet Bessel Beam Illumination microscope 

The following microscopes are optimized for mosaic scanning of large, fixed specimens on slides. They cover several different microscope types (histology, widefield fluorescence and confocal fluorescence) and are also listed by type in other tabs

Olympus VS200 whole slide scanner (drop off service)

CRi Pannoramic MIDI 20x whole slide scanner (drop off service)

Caliber I.D. RS-G4 – upright large format laser scanning confocal

MAP OF KCBD 1250

EQUIPMENT STATUS

Green: No issues. Yellow: Can be used with minor issues, see notes below. Red: Cannot be used, see notes below.

CONFOCALS

  • Leica SP5_2-photon 100% 100%
  • Marianas 100% 100%
  • SoRa Marianas 100% 100%
  • Stellaris 8 100% 100%
  • Leica SP8 100% 100%
  • Leica SP5_STED 100% 100%
  • Caliber 100% 100%
  • “Live sample” DSU 100% 100%

ANALYSIS STATIONS

  • Arivis workstation 100% 100%
  • Imaris workstation 100% 100%
  • Huygens workstation 100% 100%
  • GSD/VS200 offline workstation 100% 100%

LIGHTSHEETS

  • Lattice Lightsheet 100% 100%
  • LaVision Ultramicroscope Lightsheet 100% 100%

SCANNER DROP-OFF SERVICE

  • Olympus VS200 Slideview 100% 100%
  • CRi Pannoramic MIDI 20x 100% 100%

WIDEFIELDS

  • GSD/TIRFM 100% 100%
  • KCBD IX81 100% 100%
  • AxioObserver 7 100% 100%
  • Stereomicroscope 100% 100%
  • Axioskop 100% 100%

Lattice Lightsheet – October 28, 2024 – The LLS is back up and running. The squealing Y stage motor was swapped out for the repaired X stage motor and is on it’s way to PI for repair. The new computer arrived mid-October. We re-cabled and re-programmed the system, so everything is connected just via new COM ports. 561nm and 647nm lasers are COM 8 and COM 9, respectively. The beam finder camera is plugged into the front of the system,under the open port for external drives. The sample finder camera and the path to flip the white light LED into the path are both down, there are 4 error messages on software startup about those issues. You can skip through them and use the white button on the side of the mirror to flip it in and out manually.  New laser stack and other upgrades should arrive in December, we will email the user group as we schedule those.

Leica SP8 – Thursday 11/14 – Leica installed a loaner DMI8 base and the system is running normally for confocal. Most people can use without issues. STED users (not many of you) the loaner base does not have a chiller or a quarter wave plate, so STED will have to wait until the Z motor arrives from Germany and is installed in our regular base.  We anticipate this will take 2-3 more weeks including the holiday.

SP5 2-Photon – March 1, 2024 – The hyd D2 detector is failing. We recommend avoiding this detector is possible. For explanation of SP5 2-photon status and SP5 STED long-term deficits, please see their microscope page descriptions.