Integrated Light Microscopy Core

To access a microscope, click the New User button above to go to our New User Training Checklist.Trained users have 24/7 access to the facility and are given permission to schedule their own microscope sessions through our online scheduling software

MICROSCOPES BY TYPE AND FUNCTION

The following microscopes are capable of superresolution imaging. They cover several different microscope and superresolution types and are also listed by type in other tabs

Leica GSD/TIRFM inverted ground state depletion superresolution with TIRF

Leica SP8 inverted laser scanning confocal with white light laser, 3D STED and FLIM

3i SoRa Marianas inverted spinning disk confocal

3i Lattice Lightsheet Bessel Beam Illumination microscope 

The following microscopes are optimized for mosaic scanning of large, fixed specimens on slides. They cover several different microscope types (histology, widefield fluorescence and confocal fluorescence) and are also listed by type in other tabs

Olympus VS200 whole slide scanner (drop off service)

CRi Pannoramic MIDI 20x whole slide scanner (drop off service)

Caliber I.D. RS-G4 – upright large format laser scanning confocal

MAP OF KCBD 1250

EQUIPMENT STATUS

Green: No issues. Yellow: Can be used with minor issues, see notes below. Red: Cannot be used, see notes below.

CONFOCALS

  • Leica SP5_2-photon 100% 100%
  • Marianas 100% 100%
  • SoRa Marianas 100% 100%
  • Stellaris 8 100% 100%
  • Leica SP8 100% 100%
  • Leica SP5_STED 100% 100%
  • Caliber 100% 100%
  • “Live sample” DSU 100% 100%

ANALYSIS STATIONS

  • Arivis workstation 100% 100%
  • Imaris workstation 100% 100%
  • Huygens workstation 100% 100%
  • GSD/VS200 offline workstation 100% 100%

LIGHTSHEETS

  • Lattice Lightsheet 100% 100%
  • LaVision Ultramicroscope Lightsheet 100% 100%

SCANNER DROP-OFF SERVICE

  • Evident VS200 Slideview #1 100% 100%
  • Evident VS200 Slideview #2 100% 100%
  • CRi Pannoramic MIDI 20x 100% 100%

WIDEFIELDS

  • GSD/TIRFM 100% 100%
  • Olympus IX81 100% 100%
  • AxioObserver 7 100% 100%
  • Stereomicroscope 100% 100%
  • Axioskop 100% 100%

Lattice Lightsheet – Upgrades including new laser stack, dual cam and new laser shielding are in place. Software functions are mostly the same as before, but if you would like a quick run through before your first session please let us know in advance so someone can put you on their calendar.  

Leica SP8 – Update January 13, 2025 -Clanking sound has been resolved!

SP5 2-photon and SP5 STED – For explanations of SP5 2-photon and SP5 STED long-term deficits, please see their microscope page descriptions.

SoRa Marianas – December 9 – the LED light bar in the incubator box is malfunctioning. We have set up a temporary light bar with a push button switch. The switch location is marked on the incubator box. Imaging is fine, so once you have placed your stage insert and sample and turned off the incubator light all should be normal. Update December 23 – OKOlabs is sending a replacement LED bar, which should arrive after Winter Break.

Caliber ID – January 10, 2025 – Windows has malfunctioned on the Caliber PC.  We will attempt to restore from the December backup, but in the meantime the machine is not usable.