GLIIFCA 2025 in Chicago | September 19-20
The most awesome Flow Cytometry and Imaging regional meeting Details HerePlus the Chicago Cytek User Group Meeting on 09/18
Welcome to the Cytometry and Antibody Technology Core Facility
a Comprehensive Cancer Center Core
Cancer Center members should cite the Cancer Center Support Grant (P30CA014599) when publishing any work assisted by this core facility.
Welcome New Users!
Instructions for getting trained to use CAT Facility instruments and the available services can be found on our New User page. If you have any questions please don’t hesitate to contact the CAT Facility Staff.
Institute for Translational Medicine Core Facility Grant
The ITM wants to help you take advantage of core facility resources, awarding up to $5,000 to offset any costs associated with using one of UChicago’s Core Facilities.
Instrument Status
The CAT Facility staff works hard to ensure the instruments are working properly. In the event that there are ongoing issues with any of the instruments the status will posted at the bottom of this page.
Spectral Flow Cytometry
Get started in the spectral flow cytometry world
Blog
GLIIFCA 2025 is in Chicago!
Dear colleagues and friends, On behalf of the GLIIFCA Board of Directors, I am thrilled to invite you to the 2025 GLIIFCA Annual Meeting, taking place September 19–20 in Chicago. This marks our 34th year of cutting-edge and practical programming in flow cytometry and...
Suggested Reading: Cytometry Colibri
Very good flow cytometry blogs filled with information on sample preparation and tips and tricks on data analysis are few and far between. I strongly recommend you having a look at Cytometry Colibri from Dr. Oliver Burton from the University of Cambridge. The posts...
Setting up the voltages on your scatter plot
It's been a minute since we recorded any training material, I'm planning to be better about that this year! Here's some quick notes about the voltage settings on the FSC and SSC detectors. https://youtu.be/J0BeEkUikmg If you went through the Flow Basics class,...
Instrument Status
See notes below if bars are yellow or red. Click on each instrument to access the instrument-specific page.
- No Issues 0%
- Minor issues 0%
- Can not be used 0%
- Aurora-B
- Aurora-G
- Aurora-R
- Penteon 5-30
- Quanteon 4-25
- Fortessa 4-15
- Fortessa HTS 4-15
- Attune NxT 4-14
- CytoFLEX LX 4-16
- Bigfoot
- Aria Fusion 5-18
- AriaIII
- Tyto
- Jonas Center Quanteon
- PME Fortessa
- PME Aurora
- Image Stream MarkII
- Luminex 200
- MSD Quickplex SQ120
Fortessa 4-15 HTS issue 09/05/25 – The red laser is dead and will need to be replaced. We contacted Tech support for a quote, no replacement time has been announced yet.
Aurora-R issue 09/04/25: The violet laser output is much lower than what it should be, the signals from that detector array are unusable. The instrument should not be used for spectral flow purposes. We contacted Cytek tech support and will likely schedule a visit for a service engineer shortly.