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See our suggestions hereWelcome to the Cytometry and Antibody Technology Core Facility
Welcome New Users!
Instructions for getting trained to use CAT Facility instruments and the available services can be found on our New User page. If you have any questions please don’t hesitate to contact the CAT Facility Staff.
Institute for Translational Medicine Core Facility Grant
The ITM wants to help you take advantage of core facility resources, awarding up to $5,000 to offset any costs associated with using one of UChicago’s Core Facilities.
Instrument Status
The CAT Facility staff works hard to ensure the instruments are working properly. In the event that there are ongoing issues with any of the instruments the status will posted at the bottom of this page.
Spectral Flow Cytometry
Get started in the spectral flow cytometry world
Blog
Combinatorial Antibody Titrations
Greetings from our absolutely delightful and unseasonably warm day for February – I hope you are getting, or did get, a chance to be outside. Our temperature is going to drop 40 ° F by tomorrow….Such is weather in the Midwest. This post will cover a recent paper in...
Why the LSRII 4-12 had to go
We recently said good bye to the LSRII 4-12 and I wanted to say a few words about what happened. There are a some things to highlight here, as the retirement of these units is somewhat controversial in the field. BD had announced the end of their support of the model...
The Spectral Correction Hangover
Should I use cells or beads? This question is at the center of many discussions nowadays in the flow cytometry world. Beads were once lauded as the easy alternative to cells to prepare your compensation controls. But as more and more researchers look at their spectral...
Instrument Status
See notes below if bars are yellow or red. Click on each instrument to access the instrument-specific page.
- No Issues 0%
- Minor issues 0%
- Can not be used 0%
- Aurora-B
- Aurora-R
- Penteon 5-30
- Quanteon 4-25
- Fortessa 4-15
- Fortessa HTS 4-15
- Attune NxT 4-14
- Bigfoot
- Aria Fusion 5-18
- AriaIII
- Tyto
- Jonas Center Quanteon
- PME Fortessa
- PME Aurora
- Helios Mass Cytometer
- Image Stream MarkII
- Luminex 200
- MSD Quickplex SQ120
08/29/23 Attune NxT issue: We’re experiencing the same clog issue we’ve had all summer. It’s a very frustrating situation and we’re in discussion with TF to resolve the problem definitely. Edit 11/28/23: The loaner unit has been installed and the is ready to go. It does not have the network connection and usage tracking system featured on our other instruments so for the time being, the instrument is available free of charge, and data needs to be removed with a USB drive.
Aurora-R issue 03/11/24 : The plate loader is not moving laterally, it’s just stuck there. We have contacted Cytek about the issue and we’ll schedule a visit from a service engineer to resolve the problem. The instrument is otherwise functional, you can run samples using 5ml tubes. Just no plates, no racks. Edit 03/14/24: Somewhat surprisingly, there is no path offered by Cytek to repair the plate loader and the only option that the company is giving us is to purchase a whole new unit. Considering the cost of the plate loader and our current budgetary situation, this is not something we’ll be able to do in the immediate future, and probably not until the next fiscal year. We are looking for alternative ways to repair the unit, but we do not have a clear idea on how to proceed at this point.
AriaIII issue 03/04/24 : the instrument can not properly charge the stream and therefore can not sort the intended cells of interest. BD Biosciences has been notified and a PO has been sent out to initiate a field service engineer visit.
Bigfoot issue 03/11/24 : There is a leak around the nozzle that makes droplet formation unstable. We contacted the ThermoFisher service engineer and are expecting a visit on 03/14.