COVID 19 and CAT Facility Operation News
Updated 10/05/20 Messages from the Technical DirectorPhase 3 Plan DetailsWelcome to the Cytometry and Antibody Technology Core Facility
a Comprehensive Cancer Center Core
Cancer Center members should cite the Cancer Center Support Grant (P30CA014599) when publishing any work assisted by this core facility.
New Cell Sorter in the CAT Facility: The MACSQuant Tyto
The New MACSQuant Tyto cell sorter is specifically designed for sterility and biosafety.
NEWS
“Aria Kiddin’ Me?”: PC Troubleshooting in the CAT Facility
PC issues can be a real showstopper in a flow core, and a humbling one at that. Even for those who spend much of their workweek maintaining and repairing complicated – and expensive! – flow cytometers, fixing an unresponsive keyboard or Blue Screen of Death can be...
How to Identify Problems with Flow Cytometry Experiment Design – Bad Data Part 3
Two weeks ago I covered my red flags for identifying bad panel design (read that here if you missed it!). This week I’ll go over my red flags for experiment design, which mostly means controls. Controls are needed to be able to appropriately interpret experiment...
Instrument Status
- Aurora-B
- Aurora-G
- Fortessa X20 5-18
- Fortessa 4-15
- Fortessa HTS 4-15
- Attune NxT 4-14
- LSRII 4-12
- Accuri C6
- Aria Fusion 5-18
- AriaIIIu
- AriaII
- PME Fortessa
- PME Aurora
- Helios Mass Cytometer
- Image Stream MarkII
- Luminex 200
- MSD Quickplex SQ120
AriaIIIu issues: The computer got fried (again) and we are looking for a permanent solution to this issue. The YG laser also needs to be replaced.
Accuri C6 issue: Fluidics issue. We will replace a number a faulty valves in the hopes of resolving the problem. The instrument is no longer supported by the manufacturer, so if we can’t resolve the issue, the Accuri will be removed from the instrument roster.