Upcoming Seminar and demo
Mitlenyi Biotec CAT T cells seminar 04/23 | Tyto Lux demoFluoroFinder 05/20 | Details TBA
Welcome to the Cytometry and Antibody Technology Core Facility
a Comprehensive Cancer Center Core
Cancer Center members should cite the Cancer Center Support Grant (P30CA014599) when publishing any work assisted by this core facility.
Welcome New Users!
Instructions for getting trained to use CAT Facility instruments and the available services can be found on our New User page. If you have any questions please don’t hesitate to contact the CAT Facility Staff.
Institute for Translational Medicine Core Facility Grant
The ITM wants to help you take advantage of core facility resources, awarding up to $5,000 to offset any costs associated with using one of UChicago’s Core Facilities.
Instrument Status
The CAT Facility staff works hard to ensure the instruments are working properly. In the event that there are ongoing issues with any of the instruments the status will posted at the bottom of this page.
Spectral Flow Cytometry
Get started in the spectral flow cytometry world
Blog
Suggested Reading: Cytometry Colibri
Very good flow cytometry blogs filled with information on sample preparation and tips and tricks on data analysis are few and far between. I strongly recommend you having a look at Cytometry Colibri from Dr. Oliver Burton from the University of Cambridge. The posts...
Setting up the voltages on your scatter plot
It's been a minute since we recorded any training material, I'm planning to be better about that this year! Here's some quick notes about the voltage settings on the FSC and SSC detectors. https://youtu.be/J0BeEkUikmg If you went through the Flow Basics class,...
New Online Data Server Portal: Tips and Tricks
The new server online portal has been in use for a while and I thought we could provide a couple of tips and tricks to improve the experience. 1 - Don't use the online portal! It is slow, and the 2FA challenge gets to be annoying over time. We suggest that you use...
Instrument Status
See notes below if bars are yellow or red. Click on each instrument to access the instrument-specific page.
- No Issues 0%
- Minor issues 0%
- Can not be used 0%
- Aurora-B
- Aurora-G
- Aurora-R
- Penteon 5-30
- Quanteon 4-25
- Fortessa 4-15
- Fortessa HTS 4-15
- Attune NxT 4-14
- CytoFLEX LX 4-16
- Bigfoot
- Aria Fusion 5-18
- AriaIII
- Tyto
- Jonas Center Quanteon
- PME Fortessa
- PME Aurora
- Image Stream MarkII
- Luminex 200
- MSD Quickplex SQ120
Penteon Plate loader is fried 11/26/24: The plate loader on the Penteon won’t move properly and we’ll need to service the unit. Agilent has been contacted and we’ll have the issue resolved asap. The Penteon itself is working fine otherwise, but you’ll need to run samples using 5mL tubes.
Quanteon issue 12/20/24:The instrument is not initializing and can’t be used at this time. We swapped the plate loader to the Penteon, so the Penteon is fully functional. Agilent tech support has been contacted ,but there are some Oracle issues to deal with before we can process the PO. We hope to have the issue resolved asap, but I’m unsure when we can get a service engineer on site at this time. The issue on the Quanteon has been resolved, but we’ll leave the plate loader on the Penteon for the time being. At this time, Quanteon users will need to run samples in 5mL tubes manually. 03/11/25 update: We’ve had some issues with some parameters in the new procurement system that made it impossible to send the NovoSmpler Q unit out for repairs. This has now been resolved and we’re back on track to have it repaired. It will need to be shipped back to the manufacturer, so it will take some time still. Edit 04/29/25: The NovoSampler Q has arrived to the Vermont factory and is being assessed. We’re now waiting for an estimate for the repairs.
04/18/25 Aria Fusion issue: The waste sensor is defective and will not allow us to run the instrument. We swapped the air compressor to the AriaIII and got that one going. BD has been informed of the issue and we are waiting for a visit from a service engineer.