Cytometry and Antibody Technology

New Users

What you need to know to get your experiments started in the CAT Facility.

Share Your Publications With Us

Our mandate includes the tracking of publications showcasing work done within the CAT Facility. You can help us by sharing a link of your most recent publication here.

Spectral Flow Cytometry

Spectral Flow Cytometry is rapidly gaining popularity with flow cytometrists. While these new instruments have tremendous potential, a good understanding of the technology is required. The CAT Facility offers a library of information regarding the Cytek Aurora and analysis of spectral flow data.


Coming soon – Up-to-date training material for panel development and troubleshooting guide.

Welcome to the Cytometry and Antibody Technology Core Facility

a Comprehensive Cancer Center Core

Click here to learn more about the newest addition the the CAT Facility: the ThermoFisher Attune NxT


The new CAT Facility website!

Welcome to our new website!! Here's a quick presentation of some features that we set up. We think you will be able to navigate through the pages easily and rapidly find the information that you are looking for. the Instrument Status tool: On the Homepage is the list...

Meet the Associate Scientific Director

Along with its revival, the continuation of Ryan's blog will be getting a bit of a makeover! What’s different? The biggest change is that the main contributor of this blog will be me – Laura Johnston, Associate Scientific Director of the University of Chicago...

Welcome to the CAT Facility blog

The revamping of our website provides us with the opportunity to reboot what used to be a popular feature of the CAT Facility: the blog! We'll be posting about news from the facility, tips and tools to help with your experiments. Feel free to leave us a note in the...

Instrument Status

Green: No issues. Yellow: Can be used with minor issues, see notes below. Red: Can not be used, see notes below.

  • Aurora
  • Fortessa X20 5-18
  • Fortessa 4-15
  • Fortessa 4-15 HTS
  • Attune NxT 4-14
  • LSRII 4-12
  • Accuri C6
  • Aria Fusion 5-18
  • AriaIIIu
  • AriaII
  • Helios Mass Cytometer
  • Image Stream MarkII
  • Luminex 200
  • MSD Quickplex SQ120

Fortessa X20 5-18 issue
Valve V2 in the X20 is not clamping the sheath line, lets PBS flow back in H2O tube in Standby mode. The water eventually fills the tube and enters the air line tubing, disrupting pressure regularization.

When the instrument is not in use, it needs to be turned off.

It is otherwise working properly. Contact the CAT Facility immediately if you notice anything that seems strange.

Repairs scheduled for August 13th was cancelled by BD Service engineer

Next visit date TBD

Aria Fusion 5-18 issue
The pressure regulator is broken and the instrument won’t be usable until it is replaced. We have contacted BD and are waiting for a service engineer to schedule repairs.

Repairs scheduled for Tuesday August 20th