Cytometry and Antibody Technology

Welcome to the Cytometry and Antibody Technology Core Facility

a Comprehensive Cancer Center Core

Cancer Center members should cite the Cancer Center Support Grant (P30CA014599) when publishing any work assisted by this core facility.

Welcome New Users!

Instructions for getting trained to use CAT Facility instruments and the available services can be found on our New User page. If you have any questions please don’t hesitate to contact the CAT Facility Staff.

Institute for Translational Medicine Core Facility Grant

The ITM wants to help you take advantage of core facility resources, awarding up to $5,000 to offset any costs associated with using one of UChicago’s Core Facilities.

Instrument Status

The CAT Facility staff works hard to ensure the instruments are working properly. In the event that there are ongoing issues with any of the instruments the status will posted at the bottom of this page.

Spectral Flow Cytometry

Get started in the spectral flow cytometry world


Publications Every Flow Cytometrist Should Know About

Publications Every Flow Cytometrist Should Know About

There's a lot of information out there on the internet - some of it is good and some of it is not so good. Typically peer-reviewed publications are great, but if you're not familiar with the field it can be a bit tricky to navigate older publications and determine if...

Notes from the ABRF 2022 meeting

Laura and I attended the 2022 ABRF (Association of Biomolecular Resources Facilities) meeting. Laura's 'A Tale of Two Cytometry Platforms' on getting quality data in spectral flow was, in my opinion, the highlight of the meeting, and was very well received. She was...

Instrument Status

See notes below if bars are yellow or red. Click on each instrument to access the instrument-specific page.


  • No Issues 0% 0%
  • Minor issues 0% 0%
  • Can not be used 0% 0%
  • Aurora-B
  • Aurora-R
  • Penteon 5-30
  • Fortessa 4-15
  • Fortessa HTS 4-15
  • Attune NxT 4-14
  • LSRII 4-12
  • Bigfoot
  • Aria Fusion 5-18
  • AriaII
  • AriaIII
  • Tyto
  • Jonas Center Quanteon
  • PME Fortessa
  • PME Aurora
  • Helios Mass Cytometer
  • Image Stream MarkII
  • Luminex 200
  • MSD Quickplex SQ120
Fortessa 4-15 HTS issue: The red laser power registers at 0.3mW, likely needs to be replaced. BD has been contacted and we are waiting for the visit of a BD engineer. The instrument is otherwise working normally.

Aurora-B issue – While the top cover is closed, the signal on the FSC will periodically spread for a quick second. The QC beads look good and the instrument is otherwise working properly. We have reached to Cytek about the issue and are trying to identify the cause.

AriaII: The computer used to acquire the AriaII data crashed. We are exploring various options to get the instrument back as soon as possible. Edit 05/19 – We have been waiting on BD to send us a quote for a new computer for that AriaII, but there is some issues on their end that generates some delays. Hopefully, we’ll get things moving soon.

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