Cytometry and Antibody Technology

Happy Holiday Season!

The CAT Facility will remain open throughout the holidays with limited access to sorters between the 25th and 29th
Check the Schedule for details

Welcome to the Cytometry and Antibody Technology Core Facility

a Comprehensive Cancer Center Core

Cancer Center members should cite the Cancer Center Support Grant (P30CA014599) when publishing any work assisted by this core facility.

Welcome New Users!

Instructions for getting trained to use CAT Facility instruments and the available services can be found on our New User page. If you have any questions please don’t hesitate to contact the CAT Facility Staff.

Institute for Translational Medicine Core Facility Grant

The ITM wants to help you take advantage of core facility resources, awarding up to $5,000 to offset any costs associated with using one of UChicago’s Core Facilities.

Instrument Status

The CAT Facility staff works hard to ensure the instruments are working properly. In the event that there are ongoing issues with any of the instruments the status will posted at the bottom of this page.

Spectral Flow Cytometry

Get started in the spectral flow cytometry world


EasyPanel V2 – What’s in it for you?

We had Omar Abouzid from EasyPanel to discuss the new developments that comes with version 2. I've been pretty happy with the tool so far, but I'm now very excited to see how the new antibody inventory system will work out. The panel design tool itself has a few new...

ChUG Podcast #14: Cytometry on Air #3: Cell Sorting

The former Director of the CAT Facility, Ryan Duggan, had a good thing going with a Google Group in cytometry, before Google decided to shut down that tool. He was hosting Google hangouts, called Cytometry on Air, to discuss all things flow cytometry, which attracted...

Do you OMIP?

Here's a quick note to showcase OMIPs, a library of optimized panels published in Cytometry part A. They are a collection of peer-reviewed multi-parameters panels that include a truckload of technical details. If you're looking for a shortcut in your panel design,...

Instrument Status

See notes below if bars are yellow or red. Click on each instrument to access the instrument-specific page.


  • No Issues 0% 0%
  • Minor issues 0% 0%
  • Can not be used 0% 0%
  • Aurora-B
  • Aurora-R
  • Penteon 5-30
  • Quanteon 4-25
  • Fortessa 4-15
  • Fortessa HTS 4-15
  • Attune NxT 4-14
  • Bigfoot
  • Aria Fusion 5-18
  • AriaIII
  • Tyto
  • Jonas Center Quanteon
  • PME Fortessa
  • PME Aurora
  • Helios Mass Cytometer
  • Image Stream MarkII
  • Luminex 200
  • MSD Quickplex SQ120

New Quanteon 4-25 11/03/23: A new Quanteon has been delivered, we’ll schedule the installation with the Agilent service engineer.

11/28/23 Aria Fusion issue: The red laser is dead and needs replacement. We have contacted BD and are waiting for a service engineer to schedule a visit. The instrument is otherwise functional, just can’t run any red laser excited dyes (APC, APC-tandems, A647, etc.) 

08/29/23 Attune NxT issue: We’re experiencing the same clog issue we’ve had all summer. It’s a very frustrating situation and we’re in discussion with TF to resolve the problem definitely. Edit 11/28/23: The loaner unit has been installed and the is ready to go. It does not have the network connection and usage tracking system featured on our other instruments so for the time being, the instrument is available free of charge, and data needs to be removed with a USB drive.

11/28/23 PME Fortessa issue: The FSC detector is noisy and we have not been able to resolve the issue on our own. This prevents CST to pass. We have contacted BD tech support and are waiting for a service engineer to schedule a visit. The instrument is otherwise fully operational.

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