Cytometry and Antibody Technology

Staff Assistance

 

Members of the CAT Facility staff are always available to assist you with questions on panel design, technical issues on the instruments, or data analysis. You can also book technician time and have the staff run specific tasks on your behalf.

Experiment Troubleshooting

If you are having trouble getting good quality data, please contact us! We are available to assess the issue, determine problem area(s) and suggest a solution. Examples of this include:

  • Sample preparation – tissue digestion and antibody/dye staining
  • Panel design
  • Instrument settings (voltages)
  • Compensation

Please contact Laura Johnston and/or David Leclerc to schedule a time to meet. The first meeting is free of charge! If subsequent meetings are needed, it is likely that we will charge the consultation fee ($35/hour). This will be discussed prior to billing.

Project Management

The Associate Scientific Director is available to support your research projects on any platform in the facility. This consultation service is offered at an hourly rate of $35 for any of the topics outlined below. Alternatively, the Associate Scientific Director can be hired to run a project covering all of the services below as well as staining and running the samples.

Please email Laura Johnston to schedule a meeting. If an initial meeting is needed to assess the scope of the project, we will waive the consultation fee.

Panel Design

The Associate Scientific Director can design custom panels for all of our benchtop analyzers and sorters. This service may include:

  • Assigning fluorophores to a list of markers provided by the user
  • Rearranging an existing panel to improve data quality
  • Assigning fluorophores to markers to expand an existing panel
  • Combining smaller panels into one larger panel

On occasion, the Associate Scientific Director may suggest the addition of markers to identify common immune cell subsets of interest. However, users are typically responsible for picking their markers of interest.

Sample preparation and experimental design

The Associate Scientific Director can provide guidance on sample preparation and designing the experiment with appropriate controls. This may include:

  • Tissue digestion protocol
  • Staining protocol
  • Design of compensation controls/reference controls
  • Use of fluorescence minus one (FMO) controls, isotype controls, etc.
Data analysis and presentation

Once data has been acquired, the Associate Scientific Director can work with the user to create a custom analysis specific to the user’s project and hypothesis. This may include:

  • Checking data, panel, and compensation/spectral unmixing quality
  • Identifying cell populations of interest and differences in experimental groups
  • Determining the best method to present the findings
  • Generating publication-quality figures

Instrument assistance

You can have a member of the CAT Facility run your samples on your behalf on benchtop analyzers and multiplexing instruments.

The CAT Facility staff will run your prepared samples on the platform of your choice.

You will receive:

  • The FCS data files;
  • The analysis of your data on the analysis platform of your choice.

The CAT Facility staff can prepare the plate according to the protocol included in the kit and process the plate on the instrument.

You will receive

  • The raw data;
  • The calculated concentrations based on the readout of the standard curve.