CAT Facility Blog
Learn the latest news about the CAT Facility. Feel free to share your opinion in the comments section of each post. The comment section will be moderated for tone and content.
Many of the scientific posts are written by Laura Johnston, Associate Scientific Director. Learn more about her here.
“Aria Kiddin’ Me?”: PC Troubleshooting in the CAT Facility
PC issues can be a real showstopper in a flow core, and a humbling one at that. Even for those who spend much of their workweek maintaining and repairing complicated – and expensive! – flow cytometers, fixing an unresponsive keyboard or Blue Screen of Death can be...
How to Identify Problems with Flow Cytometry Experiment Design – Bad Data Part 3
Two weeks ago I covered my red flags for identifying bad panel design (read that here if you missed it!). This week I’ll go over my red flags for experiment design, which mostly means controls. Controls are needed to be able to appropriately interpret experiment...
How to Identify Problems with Panel Design – Bad Data Part 2
In the first post of the "bad data" series, I mostly discussed how to identify problems with cytometer settings and compensation by looking at the data plots. (Read that post here in case you missed it!) In this post I’d like to expand on that by going over common...
Acknowledging your SRL with RRID
The manner in which manuscripts acknowledge the work done in Shared Resource Laboratories (SRLs) is inconsistent and an ongoing topic of discussion. These acknowledgements are critical to the survival of SRLs as they are used to satisfy the requirements of federal...
On the Tyranny of QC Beads
The Cytek Aurora is not the most polite instrument I know. If you fail a Quality Control test (QC) when you first turn on the instrument, it will spit out a giant FAILED error message in big red letters. For the untrained users, it can be a bit much. I can tell you...
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