Learn the latest news about the CAT Facility and tips for learning flow cytometry. Feel free to share your opinion in the comments section of each post. The comment section will be moderated for tone and content.
Should I use cells or beads? This question is at the center of many discussions nowadays in the flow cytometry world. Beads were once lauded as the easy alternative to cells to prepare your compensation controls. But as more and more researchers look at their...
Edit 01/05/24: I just stumbled across a short YT video from Dr. Liston where AutoSpil is described in more specific details. I'm mad at myself. I remember seeing a bunch of tweets about something called AutoSpill back in 2021, and completely ignored it being the...
I have an unpopular opinion: I love compensation. Usually when I bring up compensation I’m met with a chorus of frustrated noises. But to me compensation is an opportunity to solve a complicated puzzle – and I really love a good puzzle. In this post I’m going to...
When calculating compensation, automated tools are the gold standard. However, people often struggle to get good results from the automated compensation tools and will turn to manual compensation to fix any errors. Why is it difficult to get accurate results...