When I was a graduate student, my PI asked me how many μL of a particular antibody I used per million cells, and I told him my staining conditions were “per 100 μL” not “per million cells”. This triggered a heated debate about antibody amount...
When working with patients, samples are rarely collected from all of the patients on the same day. That leaves two options for running samples: cells can be collected and run on the same day over the course of the study or cells can be cryopreserved and run at one...
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