Learn the latest news about the CAT Facility and tips for learning flow cytometry. Feel free to share your opinion in the comments section of each post. The comment section will be moderated for tone and content.
There's a lot of information out there on the internet - some of it is good and some of it is not so good. Typically peer-reviewed publications are great, but if you're not familiar with the field it can be a bit tricky to navigate older publications and determine...
Laura Johnston | Apr 12, 2022 | 5 Comments
With the recent addition of the NovoCyte Quanteon, the CAT Facility now employs instruments with three different classes of photodetector: Avalanche Photodiodes (APDs) on the Cytek Auroras, Silicon Photomultipliers (SiPMs) on the NovoCyte Quanteon and Penteon from...
Bert Ladd | Nov 22, 2021 | 2 Comments
I have an unpopular opinion: I love compensation. Usually when I bring up compensation I’m met with a chorus of frustrated noises. But to me compensation is an opportunity to solve a complicated puzzle – and I really love a good puzzle. In this post I’m going to...
Laura Johnston | Aug 9, 2021 | 0 Comments
I've been thinking about brightness in flow cytometry lately. It's a simple enough concept, but it's one of these things where you start thinking about it and you may quickly get lost in the numerous moving parts. One could argue that what people really care about...
David Leclerc | May 27, 2021 | 0 Comments
Two weeks ago I covered my red flags for identifying bad panel design (read that here if you missed it!). This week I’ll go over my red flags for experiment design, which mostly means controls. Controls are needed to be able to appropriately interpret experiment...
Laura Johnston | Dec 3, 2020 | 6 Comments
In the first post of the "bad data" series, I mostly discussed how to identify problems with cytometer settings and compensation by looking at the data plots. (Read that post here in case you missed it!) In this post I’d like to expand on that by going over common...
Laura Johnston | Nov 19, 2020 | 2 Comments
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