Multiplexing platforms
The CAT Facility offers two different technologies for multiplexing needs.
Model: Quickplex SQ120
Manufacturer: Meso Scale Discovery
MSD uses sandwich assays and electrochemoluminescence to measure the concentration of specific molecules in complex biological samples. Rapid plate processing is done with a cooed CCD scientific grade camera. More information on the technology can be found here.
Plate: 96 wells format available 
Number of targets: 1-10
High matrix tolerance
High 5-Log+ dynamic range
Kits available
V-Plex: validated preconfigured or custom panel of targets, high lot-to-lot consistency
U-Plex: User designed multiplex assay, using MSD reagents or your own antibodies
R-Plex: Emerging biomarkers
T-Plex:
S-Plex: High sensitivity assay that allow the detection of a single target in the low fg/mL range.
More information about the available kits in the MSD webinar hosted by the CAT facility
Complimentary analysis software available on the manufacturer’s website.
Model: Luminex 200
Manufacturer: Luminex
The Luminex employs the xMap technology to measure the concentration of various soluble molecules in biofluids. Color-coded beads capture targeted molecules in an assay sandwich are processed through a series of lasers and detectors.
Plate: 96 wells format available
Number of targets: 1-30+
Analysis software: Belysa – available on the Luminex 200 acquisition computer and the Analysis station 3.
Kits available form:
– Millipore
– ThermoFisher
– BioRad
– and more
The Luminex 200 can also be used for QuantiGene Expression Assay
Kits manufacturers may provide discount when the experiment is performed in the CAT Facility. Contact the flow lab before ordering.
Software Tutorial
Model: Mini-ELISA Plate Reader
Manufacturer: BioLegend
The ELISA plate reader is offered at no cost in the CAT Facility.
Plate: 96 wells plate
Number of targets: 1
Analysis software: Mini-Elisa plate reader app
Kits available form:
– BioLegend
– ThermoFisher
– and more
Kits manufacturers may provide discount when the experiment is performed in the CAT Facility. Contact the flow lab before ordering.
Software Tutorial
Orbital plate shaker
Orbital plate shaker in a 4C cold room
Hand held magnetic bead plate washer
BioTek ELx405 automated magnetic bead plate washer
Vacuum manifold plate washer
Vortemp 56 shaking incubator
Pricing
MSD
- Charge based on booked time in iLab.
- Full Service available – we’ll add technician time for preparation of the plate (36.06/hr). The CAT Facility will not replace kits due to technical errors from the staff.
Luminex 200
- Charge based on entries in the log book by the instrument.
- Recommend booking 1 hour per plate on iLab.
- Full Service available – we’ll add technician time for preparation of the plate (36.06/hr). The CAT Facility will not replace kits due to technical errors from the staff.