Welcome to the CAT Facility
Welcome to the Cytometry and Antibody Technology Core Facility
a Comprehensive Cancer Center Core
Cancer Center members should cite the Cancer Center Support Grant (P30CA014599) when publishing any work assisted by this core facility.
Welcome New Users!
Instructions for getting trained to use CAT Facility instruments and the available services can be found on our New User page. If you have any questions please don’t hesitate to contact the CAT Facility Staff.
Institute for Translational Medicine Core Facility Grant
The ITM wants to help you take advantage of core facility resources, awarding up to $5,000 to offset any costs associated with using one of UChicago’s Core Facilities.
Instrument Status
The CAT Facility staff works hard to ensure the instruments are working properly. In the event that there are ongoing issues with any of the instruments the status will posted at the bottom of this page.
Spectral Flow Cytometry
Get started in the spectral flow cytometry world
Blog
What on earth is CaviCon?
Earlier today, Mike blasted an email about how the CAT Facility is ditching bleach for the cleanup of its instruments. We're now going to use something we call CaviCon! Sounds like something that wants to punch Optimus Prime in the face (I learned English watching...
Combinatorial Antibody Titrations
Greetings from our absolutely delightful and unseasonably warm day for February – I hope you are getting, or did get, a chance to be outside. Our temperature is going to drop 40 ° F by tomorrow….Such is weather in the Midwest. This post will cover a recent paper in...
Why the LSRII 4-12 had to go
We recently said good bye to the LSRII 4-12 and I wanted to say a few words about what happened. There are a some things to highlight here, as the retirement of these units is somewhat controversial in the field. BD had announced the end of their support of the model...
Instrument Status
See notes below if bars are yellow or red. Click on each instrument to access the instrument-specific page.
- No Issues 0%
- Minor issues 0%
- Can not be used 0%
- Aurora-B
- Aurora-G
- Aurora-R
- Penteon 5-30
- Quanteon 4-25
- Fortessa 4-15
- Fortessa HTS 4-15
- Attune NxT 4-14
- CytoFLEX LX 4-16
- Bigfoot
- Aria Fusion 5-18
- AriaIII
- Tyto
- Jonas Center Quanteon
- PME Fortessa
- PME Aurora
- Image Stream MarkII
- Luminex 200
- MSD Quickplex SQ120
Penteon Plate loader is fried 11/26/24: The plate loader on the Penteon won’t move properly and we’ll need to service the unit. Agilent has been contacted and we’ll have the issue resolved asap. The Penteon itself is working fine otherwise, but you’ll need to run samples using 5mL tubes.
Quanteon issue 12/20/24: The instrument is not initializing and can’t be used at this time. We swapped the plate loader to the Penteon, so the Penteon is fully functional. Agilent tech support has been contacted ,but there are some Oracle issues to deal with before we can process the PO. We hope to have the issue resolved asap, but I’m unsure when we can get a service engineer on site at this time. The issue on the Quanteon has been resolved, but we’ll leave the plate loader on the Penteon for the time being. At this time, Quanteon users will need to run samples in 5mL tubes manually.
PME Fortessa issue 01/31/25: The Red laser needs to be replaced. Everything else on the instrument is fine, you just won’t be able to get signals from APC, APCCy7, etc. We contacted BD tech support and will schedule a visit form the service engineer asap.