Welcome to the CAT Facility
Welcome to the Cytometry and Antibody Technology Core Facility
a Comprehensive Cancer Center Core
Cancer Center members should cite the Cancer Center Support Grant (P30CA014599) when publishing any work assisted by this core facility.
Welcome New Users!
Instructions for getting trained to use CAT Facility instruments and the available services can be found on our New User page. If you have any questions please don’t hesitate to contact the CAT Facility Staff.
Institute for Translational Medicine Core Facility Grant
The ITM wants to help you take advantage of core facility resources, awarding up to $5,000 to offset any costs associated with using one of UChicago’s Core Facilities.
Instrument Status
The CAT Facility staff works hard to ensure the instruments are working properly. In the event that there are ongoing issues with any of the instruments the status will posted at the bottom of this page.
Spectral Flow Cytometry
Get started in the spectral flow cytometry world
Blog
The Spectral Correction Hangover
Should I use cells or beads? This question is at the center of many discussions nowadays in the flow cytometry world. Beads were once lauded as the easy alternative to cells to prepare your compensation controls. But as more and more researchers look at their spectral...
EasyPanel V2 – What’s in it for you?
We had Omar Abouzid from EasyPanel to discuss the new developments that comes with version 2. I've been pretty happy with the tool so far, but I'm now very excited to see how the new antibody inventory system will work out. The panel design tool itself has a few new...
ChUG Podcast #14: Cytometry on Air #3: Cell Sorting
The former Director of the CAT Facility, Ryan Duggan, had a good thing going with a Google Group in cytometry, before Google decided to shut down that tool. He was hosting Google hangouts, called Cytometry on Air, to discuss all things flow cytometry, which attracted...
Instrument Status
See notes below if bars are yellow or red. Click on each instrument to access the instrument-specific page.
- No Issues 0%
- Minor issues 0%
- Can not be used 0%
- Aurora-B
- Aurora-G
- Aurora-R
- Penteon 5-30
- Quanteon 4-25
- Fortessa 4-15
- Fortessa HTS 4-15
- Attune NxT 4-14
- CytoFLEX LX 4-16
- Bigfoot
- Aria Fusion 5-18
- AriaIII
- Tyto
- Jonas Center Quanteon
- PME Fortessa
- PME Aurora
- Image Stream MarkII
- Luminex 200
- MSD Quickplex SQ120
Penteon Plate loader is fried 11/26/24: The plate loader on the Penteon won’t move properly and we’ll need to service the unit. Agilent has been contacted and we’ll have the issue resolved asap. The Penteon itself is working fine otherwise, but you’ll need to run samples using 5mL tubes.
Quanteon issue 12/20/24: The instrument is not initializing and can’t be used at this time. We swapped the plate loader to the Penteon, so the Penteon is fully functional. Agilent tech support has been contacted ,but there are some Oracle issues to deal with before we can process the PO. We hope to have the issue resolved asap, but I’m unsure when we can get a service engineer on site at this time. The issue on the Quanteon has been resolved, but we’ll leave the plate loader on the Penteon for the time being. At this time, Quanteon users will need to run samples in 5mL tubes manually.
PME Fortessa issue 01/31/25: The Red laser needs to be replaced. Everything else on the instrument is fine, you just won’t be able to get signals from APC, APCCy7, etc. We contacted BD tech support and will schedule a visit form the service engineer asap.