FedEx form

Osmometer Log

New Account Request Form

Mouse BAL report

Order form

Immunostaining Sheet

Lab Order

Miele Dishwasher

Imaging experiment report

Hg Arc Lamp Hour Log

genotyping OR how to place order to Trans

Fluorescence microscope log

Ephys table

Chemical Log Template

Ephys Sheet

Biosketchform

Cell Maintain

Thawing cells

Human BAL Plating Protoco

MIN6 Cells

Mouse BAL

Plating islet cells on glass-bottom dishes

Freezing Cells

Cell Prep

Superfect Transfection TRANSFORMATION

Mini and Maxi Prep

Stripping a Membrane

Total cell membrane preparation

Isolation

Cell Lysis Protocol

Dialysis protocol

Islet isolation & perifusion summary

Isolation and fractionation of synaptic vesicles from rat brain

Protocol Isolating Mouse Islets Natalia Tamarina

Subcellular Fractionization

Growth_of_Pseudomonas_aeruginosa_Culture

TSB recipe and agar plate

Heat Shock Transformation of Chemically Competent Ecoli

genotyping OR how to place order to Trans

How to do maxi preps with ClC-3 cDNA

LIGATION

MAKING A MUTANT

Mini and Maxi Prep

PCR MUTAGENESIS STRATEGY

PRIMER DESIGN

TRANSFORMATION

1x Lockes buffer

SDS-PAGE & ECL Western Protocol

SDS-PAGE Recipes

SDS-PAGE solutions (for Bio-Rad precast gels)

SDS-PAGE solutions

1x Lockes buffer

1x TBS

Biacore and Dispensor buffers

Buffer for Lysosome acidification

CaMKII Cocktail

Citrate buffer

DESTAIN EDTA EGTA

Fixatives

GEL Mixes and other gel buffers

HBSS

LB Broth

LB plates

Phosphate buffer chart

Pyridine BUFFER

Salmonella_GFP

sigma buffer info

Solution Cheat Sheet

TBS PBS EDTA

Tris buffer chart

TSB recipe and agar plate

Western Solutions

Antibodies

ANTIBODY

CLC-3B antibodies

CLC-3B chicken antibodies

Crosslinking IgG on Dynabeads

Primary Antibodies

Primary Antibody Information

Secondary Antibodies

SecondaryAntibodies

IP short version

IP Westernblot

Phosphorylation and Kinases

In vitro Phosphorylation details

In Vitro Phosphorylation

In Vitro Phosphorylation

In Vivo Phosphorylation Materials

In Vivo Phosphorylation

Phosphorylation of Synthetic Peptides by CamKII

Phosphorylation of Synthetic Peptides

Preparation of Autonomous CaMKII

Separation AND Westernblot

SDS-PAGE & ECL Western Protocol-27axqo8

SDS-PAGE Recipes-wmgxrz

SDS-PAGE solutions (for Bio-Rad precast gels)-2drm7t7

SDS-PAGE solutions-1jjy2tt

IP Westernblot

Coomassie Protein Standard-21ll95e

Coomassie Staining-tjnvnf

Protein Standard Curve-1gt467m

GST fusion protein expression & purification DRAFT

Protocol for Microfluidics Devices Setup

Ephys Sheet (NCR)-27iw55y

IGOR macro Documentation-2bbqrpk

ITC-16 (PCI bus)-1ahew60

Manual for Amperomety Analysis-2kpkmb6

NMDG solutions-1c1u0tcClC Pulse Generator-1388h1d

ClC-Cap Pulse Generator-1gzk6j5

ClC-Time Course-sl1u8m

EPC 9 Set Up Pulse-1m6krqu

Pulse Generator Files-1vafowj

Igor

About Igor Procedures-1m890n5

Documentation-2l10bpq

About Igor Extensions-1bs0y9u

MethodsforCFTRpaper

MseNeutrophilPrep

Neutrophil_Recruitment_Thioglycollate

PseudomonPhagoAssay

Isolation and fractionation of synaptic vesicles from rat brain

Paired-recordings from synaptically coupled cortical and hippocampal neurons in acute and cultured brain slices

Postsynaptic densities prep

Preparation of Autonomous CaMKII

Preparation of organotypic hippocampal slice cultures for long-term live imaging

Preparation of Synaptic Plasma Membranes

SV acidification by acridine orange 

Synaptic Vesicle Radioactive Neurotransmitter Uptake Assay Protocol

Synaptosome isolation for EM

Bronchoalveolar_Lavage_of_Mouse_Lungs

Mouse BAL

Mouse Bronchoalveolar Lavage Protocol

Mouse Handbook-Badon

Mouse Islet Isolation Protocol

Mouse Islet Perifusion Protocol (DRAFT)

Protocol Isolating Mouse Islets 

Protocol staining Mouse Monoclonal CD8

Imaging

Confocal Double Staining

FRET

histology protocols

Long-term live imaging of neuronal circuits in organotypic hippocampal slice cultures

Quantify Fluorescence in confocal images

Staining and Labeling

Labeling Bioparticles with pHrodo and Rhodamine Green

Loading Islet Cells with Fluo-4 AM-

Protocol staining Mouse Monoclonal CD8

Staining Protocol checklist

Staining protocol for organotypic hippocampal slice cultures

Coomassie Staining

IP Westernblot

SV acidification by acridine orange 

ANTIBODY

Immunostaining of Cultured Cells

Immunostaining Sheet

Copy of pHCalibr_2006_11_22Protocol

Finalized Protocol for Endosomal Acidification Studies for Stefan

Protocol for Calibration Curve

verkman rebuttal paper

Verman detergen calibration paper

Software and Hardwares

Confocal Double Staining-2hk5kaz

Confocal from Image J to Photoshop-2mg8pyb

Confocal Tutorial-1ewae8b

Installation of HEKA and IGOR on a new computer-26disje

Osmometer Use Instructions-1y4m2wc

Quantify Fluorescence in confocal images-1z1fpqb

Osmometer contamination-2c5npzy

Phagosomal acidification measurements with confocal microscope