Welcome to the CAT Facility
Welcome to the Cytometry and Antibody Technology Core Facility
a Comprehensive Cancer Center Core
Cancer Center members should cite the Cancer Center Support Grant (P30CA014599) when publishing any work assisted by this core facility.
Welcome New Users!
Instructions for getting trained to use CAT Facility instruments and the available services can be found on our New User page. If you have any questions please don’t hesitate to contact the CAT Facility Staff.
Institute for Translational Medicine Core Facility Grant
The ITM wants to help you take advantage of core facility resources, awarding up to $5,000 to offset any costs associated with using one of UChicago’s Core Facilities.
Instrument Status
The CAT Facility staff works hard to ensure the instruments are working properly. In the event that there are ongoing issues with any of the instruments the status will posted at the bottom of this page.
Spectral Flow Cytometry
Get started in the spectral flow cytometry world
Blog
The Flood of `21 – Last update
The CAT Facility got flooded over the weekend and we want to provide as much up-to-date information about the situation and what we're doing about it. Feel free to reach out to us with any questions or concerns. Check back again here for the most recent updates....
Resolving GFP and YFP on a Fortessa using unmixing
Here's one for the Annals of Unpublishable Data. I wanted to see if using the unmixing algorithm on data acquired on traditional instruments would help resolving GFP and YFP without using a special filter set. Turns out it doesn't, and that's pretty sad. But if you...
How to Fix Flow Cytometry Compensation Errors (and Unmixing Errors) – Bad Data Part 4
I have an unpopular opinion: I love compensation. Usually when I bring up compensation I’m met with a chorus of frustrated noises. But to me compensation is an opportunity to solve a complicated puzzle – and I really love a good puzzle. In this post I’m going to walk...
Instrument Status
See notes below if bars are yellow or red. Click on each instrument to access the instrument-specific page.
- No Issues 0%
- Minor issues 0%
- Can not be used 0%
- Aurora-B
- Aurora-G
- Aurora-R
- Penteon 5-30
- Quanteon 4-25
- Fortessa 4-15
- Fortessa HTS 4-15
- Attune NxT 4-14
- CytoFLEX LX 4-16
- Bigfoot
- Aria Fusion 5-18
- AriaIII
- Tyto
- Jonas Center Quanteon
- PME Fortessa
- PME Aurora
- Image Stream MarkII
- Luminex 200
- MSD Quickplex SQ120
04/18/25 Aria Fusion issue: The waste sensor is defective and will not allow us to run the instrument. We swapped the air compressor to the AriaIII and got that one going. BD has been informed of the issue and we are waiting for a visit from a service engineer.