Welcome to the CAT Facility
Welcome to the Cytometry and Antibody Technology Core Facility
a Comprehensive Cancer Center Core
Cancer Center members should cite the Cancer Center Support Grant (P30CA014599) when publishing any work assisted by this core facility.
Welcome New Users!
Instructions for getting trained to use CAT Facility instruments and the available services can be found on our New User page. If you have any questions please don’t hesitate to contact the CAT Facility Staff.
Institute for Translational Medicine Core Facility Grant
The ITM wants to help you take advantage of core facility resources, awarding up to $5,000 to offset any costs associated with using one of UChicago’s Core Facilities.
Instrument Status
The CAT Facility staff works hard to ensure the instruments are working properly. In the event that there are ongoing issues with any of the instruments the status will posted at the bottom of this page.
Spectral Flow Cytometry
Get started in the spectral flow cytometry world
Blog
On Brightness
I've been thinking about brightness in flow cytometry lately. It's a simple enough concept, but it's one of these things where you start thinking about it and you may quickly get lost in the numerous moving parts. One could argue that what people really care about is...
“Aria Kiddin’ Me?”: PC Troubleshooting in the CAT Facility
PC issues can be a real showstopper in a flow core, and a humbling one at that. Even for those who spend much of their workweek maintaining and repairing complicated – and expensive! – flow cytometers, fixing an unresponsive keyboard or Blue Screen of Death can be...
How to Identify Problems with Flow Cytometry Experiment Design – Bad Data Part 3
Two weeks ago I covered my red flags for identifying bad panel design (read that here if you missed it!). This week I’ll go over my red flags for experiment design, which mostly means controls. Controls are needed to be able to appropriately interpret experiment...
Instrument Status
See notes below if bars are yellow or red. Click on each instrument to access the instrument-specific page.
- No Issues 0%
- Minor issues 0%
- Can not be used 0%
- Aurora-B
- Aurora-G
- Aurora-R
- Penteon 5-30
- Quanteon 4-25
- Fortessa 4-15
- Fortessa HTS 4-15
- Attune NxT 4-14
- CytoFLEX LX 4-16
- Bigfoot
- Aria Fusion 5-18
- AriaIII
- Tyto
- Jonas Center Quanteon
- PME Fortessa
- PME Aurora
- Image Stream MarkII
- Luminex 200
- MSD Quickplex SQ120
04/18/25 Aria Fusion issue: The waste sensor is defective and will not allow us to run the instrument. We swapped the air compressor to the AriaIII and got that one going. BD has been informed of the issue and we are waiting for a visit from a service engineer.