Welcome to the CAT Facility
Welcome to the Cytometry and Antibody Technology Core Facility
a Comprehensive Cancer Center Core
Cancer Center members should cite the Cancer Center Support Grant (P30CA014599) when publishing any work assisted by this core facility.
Welcome New Users!
Instructions for getting trained to use CAT Facility instruments and the available services can be found on our New User page. If you have any questions please don’t hesitate to contact the CAT Facility Staff.
Institute for Translational Medicine Core Facility Grant
The ITM wants to help you take advantage of core facility resources, awarding up to $5,000 to offset any costs associated with using one of UChicago’s Core Facilities.
Instrument Status
The CAT Facility staff works hard to ensure the instruments are working properly. In the event that there are ongoing issues with any of the instruments the status will posted at the bottom of this page.
Spectral Flow Cytometry
Get started in the spectral flow cytometry world
Blog
How to Identify Problems with Flow Cytometry Panel Design – Bad Data Part 2
In the first post of the "bad data" series, I mostly discussed how to identify problems with cytometer settings and compensation by looking at the data plots. (Read that post here in case you missed it!) In this post I’d like to expand on that by going over common...
Acknowledging your SRL with RRID
The manner in which manuscripts acknowledge the work done in Shared Resource Laboratories (SRLs) is inconsistent and an ongoing topic of discussion. These acknowledgements are critical to the survival of SRLs as they are used to satisfy the requirements of federal...
On the Tyranny of QC Beads
The Cytek Aurora is not the most polite instrument I know. If you fail a Quality Control test (QC) when you first turn on the instrument, it will spit out a giant FAILED error message in big red letters. For the untrained users, it can be a bit much. I can tell you...
Instrument Status
See notes below if bars are yellow or red. Click on each instrument to access the instrument-specific page.
- No Issues 0%
- Minor issues 0%
- Can not be used 0%
- Aurora-B
- Aurora-G
- Aurora-R
- Penteon 5-30
- Quanteon 4-25
- Fortessa 4-15
- Fortessa HTS 4-15
- Attune NxT 4-14
- CytoFLEX LX 4-16
- Bigfoot
- Aria Fusion 5-18
- AriaIII
- Tyto
- Jonas Center Quanteon
- PME Fortessa
- PME Aurora
- Image Stream MarkII
- Luminex 200
- MSD Quickplex SQ120
04/18/25 Aria Fusion issue: The waste sensor is defective and will not allow us to run the instrument. We swapped the air compressor to the AriaIII and got that one going. BD has been informed of the issue and we are waiting for a visit from a service engineer.