Welcome to the CAT Facility

Welcome to the Cytometry and Antibody Technology Core Facility
Welcome New Users!
Instructions for getting trained to use CAT Facility instruments and the available services can be found on our New User page. If you have any questions please don’t hesitate to contact the CAT Facility Staff.
Institute for Translational Medicine Core Facility Grant
The ITM wants to help you take advantage of core facility resources, awarding up to $5,000 to offset any costs associated with using one of UChicago’s Core Facilities.
Instrument Status
The CAT Facility staff works hard to ensure the instruments are working properly. In the event that there are ongoing issues with any of the instruments the status will posted at the bottom of this page.
Spectral Flow Cytometry
Get started in the spectral flow cytometry world
Blog
MACSQuant Tyto Review Part 2 – Sterile closed system high-speed cell sorting
I've been looking for sterility-designed cell sorter for a while and have organized a Tyto demo for our users in 2018 and 2019. I'd like to share what I've learned so far. Let me get some stuff out of the way before we start: 1 – I’m not thinking of the Tyto as a...
MACSQuant Tyto review part 1 – The Case for Sterility Focused Cell Sorters in Service Laboratories
Arya Stark from Game of Thrones recites a list of the names of the people she plans to murder at some point or another. I, for one, think that behavior to be odd, somewhat rude and unhealthy. In my mind, I recite a list of the features I'm looking for on cell sorters....
The Importance of Titrating Viability Dyes on the Aurora Spectral Cytometer
I wouldn't be surprised if the subject of titrating antibodies is one of the least favorite topics among users. We all know someone who skips titrations and just uses the most convenient antibody amount. But as panels get larger, antibody titration becomes much more...
Instrument Status
See notes below if bars are yellow or red. Click on each instrument to access the instrument-specific page.
- No Issues 0%
- Minor issues 0%
- Can not be used 0%
- Aurora-B
- Aurora-G
- Aurora-R
- Penteon 5-30
- Quanteon 4-25
- Fortessa 4-15
- Fortessa HTS 4-15
- Attune NxT 4-14
- CytoFLEX LX 4-16
- Bigfoot
- Aria Fusion 5-18
- AriaIII
- Tyto
- Jonas Center Quanteon
- PME Fortessa
- PME Aurora
- Image Stream MarkII
- Luminex 200
- MSD Quickplex SQ120
04/18/25 Aria Fusion issue: The waste sensor is defective and will not allow us to run the instrument. We swapped the air compressor to the AriaIII and got that one going. BD has been informed of the issue and we are waiting for a visit from a service engineer.