CytoFLEX LX

Traditional Analyzer

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Overview

The CytoFLEX LX is equipped with four lasers and an array of 16 APD detectors.

Fortessa 4-15

Model : CytoFLEX LX

Manufacturer: Beckman Coulter

Optical configuration

Located in BSLC room R022

Sample Preparation Tips

Accepted Tubes The CytoFLEX LX can accommodate any type of 5mL FACS tubes. Sample Volume If you are new to flow cytometry, it is safer to resuspend your cells (especially controls) in a larger volume. This will allow you to properly set the instrument settings without running out of sample. As you become more comfortable with the cytometer, you can use more concentrated controls so that they run faster on the instrument.

150 µL absolute minimum, 300-500 µL minimum for new users, 150-300 µL for advanced users
It’s better to bring concentrated samples, once you are at the cytometer you can easily dilute your tubes if they are running to quickly
In general, run samples at less than 35,000 events/sec

Resources

There is a lot of information available in the Resources Library (located under the Resources menu)! The ones relevant to the Penteon/Quanteon can also be found in the module below.